Dissecting the link between stress fibres and focal adhesions by CALI wit EGFP fusion proteins

Nature Cell Biology

Volumn 4, Issue 4, 2002, Pages 286-293

  Rajfur, Zenon ^a   Roy, Partha ^a   Otey, Carol ^a   Romer, Lewis ^a   Jacobson, Ken ^a  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; ALPHA ACTININ; BETA1 INTEGRIN; FOCAL ADHESION KINASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PAXILLIN; PROTEIN SUBUNIT; REAGENT;

ANIMAL CELL; ARTICLE; CELL DAMAGE; CELL STRAIN 3T3; CELL STRUCTURE; CHROMATOPHORE; CONTROLLED STUDY; CYTOPLASM; CYTOSKELETON; ENZYME ACTIVITY; ENZYME BINDING; FOCAL ADHESION; IN VITRO STUDY; LASER; MICROFILAMENT; MOUSE; NONHUMAN; PHASE CONTRAST MICROSCOPE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN ISOLATION; SIGNAL TRANSDUCTION; STRESS FIBER;

3T3 CELLS; ACTININ; ANIMALS; ANTIGENS, CD29; BLOTTING, WESTERN; CELL ADHESION; CELL LINE; CYTOLOGICAL TECHNIQUES; CYTOSKELETON; FOCAL ADHESION KINASE 1; FOCAL ADHESION PROTEIN-TYROSINE KINASES; GREEN FLUORESCENT PROTEINS; INTEGRINS; LASERS; LUMINESCENT PROTEINS; MICE; MODELS, BIOLOGICAL; PHOSPHORYLATION; PRECIPITIN TESTS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN-TYROSINE KINASES; RECOMBINANT FUSION PROTEINS; STRESS FIBERS; TIME FACTORS;

ANIMALIA;

EID: 0036228954     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/ncb772     Document Type: Article

References (30)

1. Chromophore-assisted laser inactivation (CALI): Probing protein function in situ with a high degree of spatial and temporal resolution
2. Selective destruction of protein function by chromophore-assisted laser inactivation
3. Chromophore-assisted laser inactivation of proteins is mediated by the photogeneration of free radicals
4. Chromophore-assisted laser inactivation of patched protein switches cell fate in the larval visual system of Drosophila
5. Essential functions of ezrin in maintenance of cell shape and lamellipodial extension in normal and transformed fibroblasts
6. Asymmetric retraction of growth cone filopodia following focal inactivation of calcineurin
7. Chromophore-assisted light inactivation and self-organization of microtubules and motors
8. Alpha-actinin: Immunofluorescent localization of a muscle structural protein in nonmuscle cell
9. Inhibition of focal adhesion kinase (FAK) signalling in focal adhesions decreases cell motility and proliferation
10. pp125FAK in the focal adhesion
11. Dynamics of α-actinin in focal adhesions and stress fibres visualized with α-actinin-green fluorescent protein
12. pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions
13. Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin
14. Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: A role in cytoskeletal assembly
15. Regulation of focal adhesion-associated protein tyrosine kinase by both cellular adhesion and oncogenic transformation
16. Cell adhesion or integrin clustering increases phosphorylation of a focal adhesion-associated tyrosine kinase
17. Tyrosine kinase activity, cytoskeletal organization, and motility in human vascular endothelial cells
18. A mechanism for regulation of the adhesion-associated protein tyrosine kinase pp125FAK
19. Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice
20. Biochemical signals and biological responses elicited by the focal adhesion kinase
21. Paxillin binding is not sole determinant of focal adhesion localization or dominant-negative activity of focal adhesion kinase/focal adhesion kinase-related nonkinase
22. Alpha-actinin: A direct link between actin and integrins
23. 1 integrin cytoplasmic domain
24. 2 cytoplasmic tail
25. Restructuring of focal adhesion plaques by PI 3-kinase: Regulation by PtdIns(3,4,5)-P3 binding to α-actinin
26. 1 integrin subunit in vitro
27. 2-subunit (CD18) and the actin binding protein α-actinin
28. Disruption of the actin cytoskeleton after microinjection of proteolytic fragments of α-actinin
29. Focal adhesions, contractility, and signalling
30. Fluorescence redistribution after photobleaching. A new multipoint analysis of membrane translational dynamics