Expression of the prodrug-activating enzyme DT-diaphorase via Ad5 delivery to human colon carcinoma cells in vitro

Cancer Gene Therapy

Volumn 9, Issue 2, 2002, Pages 209-217

  Misra, Veet ^a   Klamut, Henry J ^a   Rauth, A M ^a  

^a PRINCESS MARGARET HOSPITAL   (Canada)

Author keywords

Adenovirus (Ad5); BE; Clonal assay; DT diaphorase (DTD); HT 29; Mitomycin C (MMC)

Indexed keywords

ADENOVIRUS VECTOR; COMPLEMENTARY DNA; MITOMYCIN C; NUCLEOTIDE; PRODRUG; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) DEHYDROGENASE (QUINONE);

ARTICLE; COLON CARCINOMA; COLONY FORMING UNIT; CONTROLLED STUDY; CYTOMEGALOVIRUS; ENZYME ACTIVITY; GENE EXPRESSION; GENE TRANSFER; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION;

ADENOVIRIDAE; CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS;

EID: 0036168223     PISSN: 09291903     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cgt.7700430     Document Type: Article

References (43)

1. Towards selectivity? Approaches to the design of new anti-tumour agents - I and II
2. Selectivity - Key to chemotherapy: Presidential address
3. Discovering novel chemotherapeutic drugs for the third millennium
4. Cellular approaches to bioreductive drug mechanisms
5. Prodrug-activating systems in suicide gene therapy
6. Prodrug activation enzymes in cancer gene therapy
7. The choice of prodrugs for gene-directed enzyme prodrug therapy of cancer
8. Sensitization of human carcinoma cells to the prodrug CB1954 by adenovirus vector-mediated expression of E. coli nitroreductase
9. Bioreductive therapies: An overview of drugs and their mechanisms of action
10. DT-diaphorase: Possible roles in cancer chemotherapy and carcinogenesis
11. DT-diaphorase: A historical review
12. Enzyme-directed bioreductive drug development revisited: A commentary on recent progress and future prospects with emphasis on quinone anticancer agents and quinone metabolizing enzymes, particularly DT-diaphorase
13. DT-diaphorase and cytochrome B5 reductase in human lung and breast tumours
14. DT-diaphorase activity in NSCLC and SCLC cell lines: A role for fos/jun regulation
15. NAD(P)H: Quinone oxidoreductase gene expression in human colon carcinoma cells: Characterization of a mutation which modulates DTD activity and mitomycin sensitivity
16. Exploring the mechanistic aspects of mitomycin antibiotic bioactivation in Chinese hamster ovary cells overexpressing NADPH: Cytochrome C (P-450) reductase and DT-diaphorase
17. Induction of DTD in cancer chemoprevention and chemotherapy
18. Rapid polyubiquitination and proteasomal degradation of a mutant form of NAD(P)H: Quinone oxidoreductase 1
19. Assessment of the relationship between genotypic status of a DT-diaphorase point mutation and enzymatic activity
20. Cytotoxic effects of AD5CMV-p53 expression in two human nasopharyngeal carcinoma cell lines
21. Transfection of COS-1 cells with DT-diaphorase cDNA: Role of a base change at position 609
22. A simple technique for the rescue of early region I mutations into infectious human adenovirus type 5
23. A new technique for the assay of infectivity of human adenovirus 5 DNA
24. Manipulation of adenovirus vectors
25. Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos
26. Tumor suppressor genes
27. A new screening system for NAD(P)H: Quinone oxidoreductase (NQO1)-directed antitumour quinones: Identification of a new aziridinylbenzoquinone, RH1, as a NQO1-directed antitumor agent
28. DTD activity and mitomycin C sensitivity in non-transformed cell strains derived from members of a cancer-prone family
29. Reductase enzyme expression across the National Cancer Institute tumor cell line panel: Correlation with sensitivity to mitomycin C and EO9
30. NAD(P)H: Quinone oxidoreductase1 (DT-diaphorase) expression in normal and tumor tissues
31. Nicotinamide adenine dinucleotide (phosphate): Quinone oxidoreductase (DT-diaphorase) as a target for bioreductive antitumor quinones: Quinone cytotoxicity and selectivity in human lung and breast cancer cell lines
32. NAD(P)H: Quinoneoxidoreductase: Polymorphisms and allele frequencies in Caucasian, Chinese and Canadian Native Indian and Inuit populations
33. Sensitisation of human ovarian cancer cells to killing by the prodrug CB1954 following retroviral or adenoviral transfer of the E. coli nitroreductase gene
34. Gene therapy of experimental malignant mesothelioma using adenovirus vectors encoding the HSVtk gene
35. Gene therapy of metastatic colon carcinoma: Regression of multiple hepatic metastases by adenoviral expression of bacterial cytosine deaminase
36. Factors influencing the induction of DT-diaphorase activity by 1,2-dithiole-3-thione in human tumor cell lines
37. Relationship between NAD(P)H: Quinone oxidoreductase 1 (NQO1) levels in a series of stably transfected cell lines and susceptibility to antitumor quinones
38. In suicide gene therapy, the site of subcellular localization of the activating enzyme is more important than the rate at which it activates prodrug
39. Establishment of an isogenic human colon tumor model for NQO1 gene expression: Application to investigate the role of DT-diaphorase in bioreductive drug activation in vitro and in vivo
40. Cancer gene therapy: Hard lessons and new courses
41. Molecular basis of the catalytic differences among DT-diaphorase of human, rat, and mouse
42. Demonstration of the activation of prodrug CB 1954 using human DT-diaphorase mutant Q104Y-transfected MDA-MB-231 cells and mouse xenograft model