Yeast U1 snRNP-pre-mRNA complex formation without U1snRNA-pre-mRNA base pairing

RNA

Volumn 7, Issue 1, 2001, Pages 133-142

  Du, Hansen ^a   Rosbash, Michael ^a  

^a BRANDEIS UNIVERSITY   (United States)

Author keywords

5 Splice site; Base pairing; Stability; Yeast commitment complex

Indexed keywords

MESSENGER RNA;

ANIMAL CELL; ARTICLE; BASE PAIRING; HELA CELL; HUMAN; HUMAN CELL; MAMMAL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROCESSING; RNA SPLICING; SPLICEOSOME; YEAST;

5' UNTRANSLATED REGIONS; BASE PAIRING; BASE SEQUENCE; BINDING SITES; CROSS-LINKING REAGENTS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; RIBONUCLEASE H, CALF THYMUS; RIBONUCLEOPROTEIN, U1 SMALL NUCLEAR; RNA PRECURSORS; RNA, FUNGAL; RNA, SMALL NUCLEAR; SACCHAROMYCES CEREVISIAE; TRIOXSALEN;

ANIMALIA; MAMMALIA;

EID: 0035037961     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838201001844     Document Type: Article

References (52)

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19. Universally conserved and yeast-specific U1 snRNA sequences are important but not essential for U1 snRNP function
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21. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway
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26. Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes
27. Interaction of the U1 snRNP with nonconserved intronic sequences affects 5′ splice site selection
28. A minimal spliceosomal complex A recognizes the branch site and polypyrimidine tract
29. Initial splice-site recognition and pairing during pre-mRNA splicing
30. Spliceosome assembly
31. Involvement of U1 small nuclear ribonucleoproteins (snRNP) in 5′ splice site-U1 snRNP interaction
32. Specific and stable intron-factor interactions are established early during in vitro pre-mRNA splicing
33. Yeast pre-mRNA splicing
34. A U1 snRNA: Pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5′ cleavage site
35. Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing
36. 5′ splice site selection in yeast: Genetic alterations in base-pairing with U1 reveal additional requirements
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38. More than half of yeast U1 snRNA is dispensable for growth
39. Genome-wide bioinformatic and molecular analysis of introns in Saccharomyces cerevisiae
40. Mechanical devices of the spliceosome: Motors, clocks, springs, and things
41. An RNA switch at the 5′ splice site requires ATP and the DEAD box protein Prp28p
42. Splicing takes a Holliday
43. Identification and characterization of the yeast homologue of U1 snRNP-specific protein C
44. SR proteins can compensate for the loss of U1 snRNP functions in vitro
45. Highly diverged U4 and U6 small nuclear RNAs required for splicing rare AT-AC introns
46. A novel role for a U5 snRNP protein in 3′ splice site selection
47. Intrinsic U2AF binding is modulated by exon enhancer signals in parallel with changes in splicing activity
48. Protein functions in pre-mRNA splicing
49. In vitro reconstitution of mammalian U1 snRNPs active in splicing: The U1-C protein enhances the formation of early (E) spliceosomal complexes
50. Identification of eight proteins that crosslink to pre-mRNA in the yeast commitment complex
51. A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation
52. The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing