A multiplex PCR for identifying Shiga-like toxin-producing Escherichia coli O157:H7

Letters in Applied Microbiology

Volumn 24, Issue 3, 1997, Pages 172-176

  Meng, J ^a   Zhao, S ^a   Doyle, M P ^a   Mitchell, S E ^a   Kresovich, S ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FOOD MICROBIOLOGY; GENE ENCODING; POLYMERASE CHAIN REACTION;

BACTERIAL STRAINS; CONFIRMATION TEST; E. COLI; E.COLI O157:H7; ESCHERICHIA COLI O157:H7; GENES ENCODING; PCR ASSAY; PRIMERS DESIGN; SHIGA-LIKE TOXINS;

ESCHERICHIA COLI;

SHIGA TOXIN;

ARTICLE; BACTERIUM IDENTIFICATION; DIAGNOSTIC TEST; ESCHERICHIA COLI; FOOD CONTAMINATION; FOOD CONTROL; HUMAN; NONHUMAN; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030934650     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.1997.00375.x     Document Type: Article

References (17)

1. Begum, D., Strockbine, N.A., Sowers, E.G. and Jackson M.P. (1993) Evaluation of a technique for identification of Shiga-like toxin producing Escherichia coli by using polymerase chain reaction and digoxigenin-labeled probes. Journal of Clinical Microbiology 31, 3153-3156.
2. Brian, M.J., Frosolono, M. and Murray, B.E. (1992) Polymerase chain reaction for diagnosis of enterohemorrhagic Escherichia coli infection and haemolytic uremic syndrome. Journal of Clinical Microbiology 30, 1801-1806.
3. Chamberlain, J.S. and Chamberlain, J.R. (1994) Optimization of multiplex PCRs. In The Polymerase Chain Reaction ed. Mullis, K.B., Ferré, F. and Gibbs, R.A. pp. 38-46. Boston: Birkhäuser.
4. Cebula, T.A., Payne, W.L. and Feng, P. (1995) Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. Journal of Clinical Microbiology 33, 248-250.
5. Doyle, M.P. (1991) Escherichia coli O157:H7 and its significance in foods. International Journal of Food Microbiology 12, 289-301.
6. Fratamico, P., Solomon, K.S., Wiedmann, M. and Deng, M.Y. (1995) Detection of Escherichia coli O157:H7 by multiplex PCR. Journal of Clinical Microbiology 33, 2188-2191.
7. Gannon, V.P.J., Rashed, M., King, R.K. and Golsteyn Thomas, E.J. (1992) Detection and characterization of the eae gene of Shiga-like toxin-producing Escherichia coli using polymerase chain reaction. Journal of Clinical Microbiology 31, 1268-1274.
8. Griffin, P.M. (1995) Escherichia coli O157:H7 and other enterohemorrhagic Escherichia coli. In Injections of Gastrointestinal Tract ed. Blaser, M.J., Smith, P.D., Ravdin, J.I., Greenberg, H.B. and Guerrant, R.L. pp. 739-761. New York: Raven Press.
9. Meng, J., Doyle, M.P., Zhao, T. and Zhao, S. (1994) Detection and control of Escherichia coli O157:H7 in foods. Trends in Food Science and Technology 5, 179-185.
10. Meng, J., Zhao, S., Zhao, T. and Doyle, M.P. (1995a) Molecular characterization of Escherichia coli O157:H7 isolates from raw milk, ground beef, and calf faeces using pulsed field gel electrophoresis and plasmid DNA analysis. Journal of Medical Microbiology 42, 258-263.
11. Meng, J., Zhao, S., Doyle, M.P., Mitchell, S.E. and Kresovich, S. (1995b) Polymerase chain reaction for detecting Escherichia coli O157:H7, abstr. P-68, p. 394, Abstract 95th Annual Meeting American Society for Microbiology, Washington, DC.
12. Paton, A.W., Paton, J.C., Goldwater, P.N. and Manning, P.A. (1993) Direct detection of Escherichia coli Shiga-like genes in primary faecal cultures by polymerase chain reaction. Journal of Clinical Microbiology 31, 3063-3067.
13. Pollard, D.R., Johnson, W.M., Lior, H., Tayler, S.D. and Rozee, K.R. (1990) Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction. Journal of Clinical Microbiology 28, 540-545.
14. Read, S.C., Clarke, R.C., Martin, A., De Grandis, S.A., Hill, J., McEwen, S. and Gyles, C.L. (1992) Polymerase chain reaction for detection of verocytotoxigenic Escherichia coli isolated from animal and food sources. Molecular and Cellular Probes 6, 153-161.
15. Rolfs, A., Schuller, I., Finckh, U. and Weber-Rolfs, I. (1992) PCR: principles and reaction components. In PCR: Clinical Diagnostics and Research ed. Rolfs, A., Schuller, I, Finckh, U. and Weber-Rolfs, I. pp. 1-16. Berlin: Springer-Verlag.
16. Whittam, T.S., Wolfe, M.L., Wachsmuth, I.K., Orskov, F., Orskov, I. and Wilson, R.A. (1993) Clonal relationships among Escherichia coli strains that cause haemorrhagic colitis and infantile diarrhoea. Infection and Immunity 61, 1619-1629.
17. Zhao, S., Mitchell, S.E., Meng, J., Doyle, M.P. and Kresovich, S. (1995) Cloning and nucleotide sequence of a gene upstream of the eaeA gene of enterohemorrhagic Escherichia coli O157:H7. FEMS Microbiology Letters 133, 35-39.