Antibody-direct epifluorescent filter technique and immunomagnetic separation for 10-h screening and 24-h confirmation of Escherichia coli O157:H7 in beef

Journal of Food Protection

Volumn 59, Issue 10, 1996, Pages 1072-1075

  Restaino, Lawrence ^a   Castillo, H Jeffrey ^a   Stewart, Diana ^b   Tortorello, Mary Lou ^b  

^a R and F Laboratories   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

beef; E. coli O157:H7; epifluorescence; immunomagnetic separation; rapid screening

Indexed keywords

ESCHERICHIA COLI;

EID: 0029910613     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X-59.10.1072     Document Type: Article

References (10)

1. Centers for Disease Control. 1993. Update: multistate outbreak of Escherichia coli O157:H7 infections from hamburgers - western United States, 1992-1993. Morbid. Mortal. Weekly Rep. 42:258-263.
2. Johnson, J. L., B. E. Rose, A. K. Sharar, G. M. Ransom, C. P. Lattuada, and A. M. McNamara. 1995. Methods used for detection and recovery of Escherichia coli 0157:H7 associated with a food-borne disease outbreak. J. Food Prot. 58:597-603.
3. Meng, J., M. P. Doyle, T. Zhao, and S. Zhao. 1994. Detection and control of Escherichia coli O157:H7 in foods. Trends Food Sci. Technol. 5:179-185.
4. Okrend, A. J. G., and B. E. Rose. 1989. Isolation and identification of Escherichia coli O157:H7 from meat. Revision 3, laboratory communication no. 38. FSIS, Microbiology Division, U.S. Department of Agriculture, Washington, D.C.
5. Okrend, A. J. G., B. E. Rose, and B. Bennett. 1990. A screening method for the isolation of Escherichia coli O157:H7 from ground beef. J. Food Prot. 53:249-252.
6. Padhye, N. V., and M. P. Doyle. 1991. Rapid procedure for detecting enterohemorrhagic Escherichia coli O157:H7 in food. Appl. Environ. Microbiol. 57:2693-2698.
7. Przybyski, K. S., and L. D. Witter. 1979. Injury and recovery of Escherichia coli after sublethal acidification. Appl. Environ. Microbiol. 37:261-265.
8. Tortorello, M. L., and D. S. Stewart. 1994. Antibody-direct epifluorescent filter technique for rapid, direct enumeration of Escherichia coli O157:H7 in beef. Appl. Environ. Microbiol. 60:3553-3559.
9. Weagant, S. D., J. L. Bryant and K. G. Jinneman. 1995. An improved rapid technique for isolation of Escherichia coli O157:H7 from foods. J. Food Prot. 58:7-12.
10. Wright, D. J., P. A. Chapman, and C. A. Siddons. 1994. Immunomagnetic separation as a sensitive method for isolating Escherichia coli 0157 from food samples. Epidemiol. Infect. 113:31-39.