Distribution of acridine orange fluorescence in Plasmodium falciparum-infected erythrocytes and its implications for the evaluation of digestive vacuole pH

Molecular and Biochemical Parasitology

Volumn 119, Issue 2, 2002, Pages 301-304

  Bray, Patrick G ^a   Saliba, Kevin J ^b   Davies, Jill D ^a   Spiller, David G ^c   White, Michael R H ^c   Kirk, Kiaran ^b   Ward, Stephen A ^a  

^a LIVERPOOL SCHOOL OF TROPICAL MEDICINE   (United Kingdom)

^b AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^c UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

Acridine orange; pH; SNARF dextran; Vacuole

Indexed keywords

ACRIDINE ORANGE; ANTIMALARIAL AGENT; CHLOROQUINE; GENE PRODUCT; VERAPAMIL;

ARTICLE; CELL MEMBRANE; CELL VACUOLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DIAGNOSTIC VALUE; ERYTHROCYTE; EVALUATION; FEEDING; FLUORESCENCE; MALARIA; NONHUMAN; PH; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; QUANTITATIVE ASSAY; STAINING; TONOPLAST; TROPHOZOITE; VALIDATION PROCESS;

ACRIDINE ORANGE; ANIMALS; BENZOPYRANS; CHLOROQUINE; CYTOSOL; DRUG RESISTANCE; ERYTHROCYTES; FLUORESCENCE; FLUORESCENT DYES; FLUOROMETRY; HUMANS; HYDROGEN-ION CONCENTRATION; MICROSCOPY, CONFOCAL; PLASMODIUM FALCIPARUM; REPRODUCIBILITY OF RESULTS; SCATTERING, RADIATION; VACUOLES;

PLASMODIUM FALCIPARUM;

EID: 0036155237     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(01)00403-0     Document Type: Article

References (13)

1. Cellular uptake of chloroquine is dependent on binding to ferriprotoporphyrin IX and is independent of NHE activity in Plasmodium falciparum
2. A P-glycoprotein homologue of Plasmodium falciparum is localized on the digestive vacuole
3. Mutations in the P. falciparum digestive vacuole transmembrane protein PfCRT and evidence for their role in chloroquine resistance
4. Lysosomes, pH and the anti-malarial action of chloroquine
5. Susceptibility of human malaria parasites to chloroquine is pH dependent
6. Chloroquine uptake by Plasmodium falciparum-infected human erythrocytes during in vitro culture and its relationship to chloroquine resistance
7. Kinetic modelling of the response of Plasmodium falciparum to chloroquine and its experimental testing in vitro. Implications for mechanism of action of and resistance to the drug
8. Digestive vacuolar pH of intact intraerythrocytic P. falciparum either sensitive or resistant to chloroquine
9. The effects of chloroquine and verapamil on digestive vacuolar pH of P. falciparum either sensitive or resistant to chloroquine
10. Alkalinization of the food vacuole of malaria parasites by quinoline drugs and alkylamines is not correlated with their antimalarial activity
11. Antimalarials increase vesicle pH in Plasmodium falciparum
12. +-ATPase