Hydration effects of heparin on antithrombin probed by osmotic stress

Biophysical Journal

Volumn 82, Issue 2, 2002, Pages 1040-1049

  McGee, Maria P ^a   Liang, Jie ^b   Luba, James ^a  

^a WAKE FOREST SCHOOL OF MEDICINE   (United States)

^b University of Illinois at Chicago   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTITHROMBIN; HEPARIN; PROTEINASE;

AMINO ACID COMPOSITION; ARTICLE; BINDING KINETICS; CONFORMATIONAL TRANSITION; ENERGY TRANSFER; ENZYME CONFORMATION; HYDRATION; MOLECULAR DYNAMICS; OSMOTIC STRESS; PROTEIN PROTEIN INTERACTION;

INSERTION SEQUENCES;

EID: 0036151757     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(02)75463-5     Document Type: Article

References (45)

1. Mechanisms of poly(ethylene glycol) interaction with proteins
2. Mechanism of precipitation of proteins by polyethylene glycols: Analysis in term of excluded volume
3. Antithrombins Wibble and Wobble (T85M/K): Archetypal conformational diseases with in vivo latent-transition, thrombosis, and heparin activation
4. The dynamics of water-protein interactions
5. Mobile reactive centre of serpins and the control of thrombosis
6. Protein solvation in allosteric regulation: A water effect on hemoglobin
7. Analytical molecular surface calculations
8. Transient kinetics of heparin-catalyzed protease inactivation by antithrombin III: Characterization of assembly, product formation, and heparin dissociation steps in the factor Xa reaction
9. Mechanism of heparin activation of antithrombin: Evidence for an induced-fit model of allosteric activation involving two interaction subsites
10. Osmotic regulation of gene action
11. Identification of the antithrombin III heparin binding site
12. Serine 380 (P14) → glutamate mutation activates antithrombin as an inhibitor of factor Xa
13. Excluded-volume theory of polymer-protein interactions based on polymer chain statistics
14. Mechanism of heparin activation of antithrombin: Evidence for reactive loop preinsertion with expulsion upon heparin binding
15. The anticoagulant activation of antithrombin by heparin
16. The kinetics of hemostatic enzyme-antithrombin interactions in the presence of low molecular weight heparin
17. Polymer-protein interactions: Comparison of experiment and excluded volume theory
18. Pore size distribution analysis of gel substances by size exclusion chromatography
19. Inhibitory mechanisms of serpins: Loop insertion forces acylation of plasminogen activator by plasminogen activator inhibitor 1
20. Antithrombin and its deficiency states
21. The serpin-proteinase complex revealed
22. Characterization of a human α 1-antitrypsin variant that is as stable as ovalbumin
23. Analytical shape computation of macromolecules: I. Molecular area and volume through alpha shape
24. Anatomy of protein pockets and cavities: Measurement of binding site geometry and implications for ligand design
25. Hydration structure of antithrombin conformers and water transfer during reactive loop insertion
26. Protein hydration changes during catalysis: A new mechanism of enzymic rate-enhancement and ion activation/inhibition of catalysis
27. Deconvolution of the fluorescence emission spectrum of human antithrombin and identification of the tryptophan residues that are responsive to heparin binding
28. Kinetic characterization of heparin-catalyzed and uncatalyzed inhibition of blood coagulation proteinases by antithrombin
29. Binding of high affinity heparin to antithrombin III. Characterization of the protein fluorescence enhancement
30. Osmotic stress for the direct measurement of intermolecular forces
31. Osmotic stress, crowding, preferential hydration, and binding: A comparison of perspectives
32. Oligosaccharide sequences of endothelial cell surface heparan sulfate proteoglycan with affinity for lipoprotein lipase
33. Raising water to new heights
34. On the size of active sites in proteases
35. The 2.6 Å structure of antithrombin indicates a conformational change at the heparin binding site
36. Implications for function and therapy of a 2.9 Å structure of binary-complexed antithrombin
37. The intact and cleaved human antithrombin III complex as a model for serpin-proteinase interaction
38. Formation of the covalent serpinproteinase complex involves translocation of the proteinase by more than 70 Å and full insertion of the reactive center loop into β-sheet A
39. Functional requirements for inhibition of thrombin by antithrombin III in the presence and absence of heparin
40. Structural insights into serpin-protease complexes reveal the inhibitory mechanisms of serpins
41. Formation of antithrombin heterodimer in vivo and the onset of thrombosis