E4orf6 variants with separate abilities to augment adenovirus replication and direct nuclear localization of the E1B 55-kilodalton protein

Journal of Virology

Volumn 76, Issue 3, 2002, Pages 1475-1487

  Orlando, Joseph S ^a   Ornelles, David A ^a  

^a WAKE FOREST SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOVIRUS; ALPHA HELIX; AMINO ACID ANALYSIS; AMINO ACID SUBSTITUTION; ARTICLE; CELL NUCLEUS; DELETION MUTANT; HELA CELL; HUMAN; HUMAN CELL; IMMUNOBLOTTING; LIGAND BINDING; MOLECULAR DYNAMICS; MOLECULAR MODEL; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; SEQUENCE ANALYSIS; THEORETICAL MODEL; VIRION; VIRUS INFECTION; VIRUS REPLICATION;

EID: 0036147328     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.76.3.1475-1487.2002     Document Type: Article

References (57)

1. DNA-damaging agents greatly increase the transduction of nondividing cells by adeno-associated virus vectors
2. Characterization of an adenovirus gene transfer vector containing an E4 deletion
3. Suppression of human colorectal carcinoma cell growth by wild-type p53
4. 14-3-3 Proteins and growth control
5. The N terminus of Nef from HIV-1/SIV associates with a protein complex containing Lck and a serine kinase
6. Analysis of synthesis, stability, phosphorylation, and interacting polypeptides of the 34-kilodalton product of open reading frame 6 of the early region 4 protein of human adenovirus type 5
7. A rapid and highly efficient method for PCR-based site-directed mutagenesis using only one new primer
8. Adenovirus E4 34k and E4 11k inhibit double strand break repair and are physically associated with the cellular DNA-dependent protein kinase
9. Genetic analysis of a potential zinc-binding domain of the adenovirus E4 34k protein
10. The nuclear export signal within the adenovirus E4orf6 protein contributes to several steps in the viral life cycle
11. Interaction of adenoviral E4 and E1b products in late gene expression
12. Redundant control of adenovirus late gene expression by early region 4
13. Structure of the adenovirus E4 Orf6 protein predicted by fold recognition and comparative protein modeling
14. Empirical predictions of protein conformation
15. Nuclear export of the E1B 55-kDa and E4 34-kDa adenoviral oncoproteins mediated by a rev-like signal sequence
16. Blockage by adenovirus E4orf6 of transcriptional activation by the p53 tumor suppressor
17. Nuclear export of adenovirus RNA
18. The adenovirus type 5 E1B-55K oncoprotein actively shuttles in virus-infected cells, whereas transport of E4orf6 is mediated by a CRM1-independent mechanism
19. 14-3-3 Proteins: Structure, function, and regulation
20. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase
21. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle
22. p53 status does not determine outcome of E1B 55-kilodalton mutant adenovirus lytic infection
23. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells
24. Characteristics of a human cell line transformed by DNA from human adenovirus type 5
25. Overexpression of cyclin A inhibits augmentation of recombinant adeno-associated virus transduction by the adenovirus E4orf6 protein
26. Adenovirus early region 4 encodes functions required for efficient DNA replication, late gene expression, and host cell shutoff
27. Adenovirus E4orf6 oncoprotein modulates the function of the p53-retated protein, p73
28. Adenovirus early region 4 encodes two gene products with redundant effects in lytic infection
29. Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells
30. Isolation of deletion and substitution mutants of adenovirus type 5
31. Complementation of adenovirus E4 mutants by transient expression of E4 cDNA and deletion plasmids
32. The adenovirus type 5 E1B-55K oncoprotein is a highly active shuttle protein and shuttling is independent of E4orf6, p53 and Mdm2
33. Elucidating the folding problem of alpha-helices: Local motifs, long-range electrostatics, ionic-strength dependence and prediction of NMR parameters
34. E4 gene function in adenovirus, adenovirus vector and adeno-associated virus infections
35. Crystal structure of the zeta isoform of the 14-3-3 protein
36. Crystal structure of the unique RNA-binding domain of the influenza virus NS1 protein
37. The adenovirus E4 17-kilodalton protein complexes with the cellular transcription factor E2F, altering its DNA-binding properties and stimulating E1A-independent accumulation of E2 mRNA
38. Oncogenic potential of the adenovirus E4orf6 protein
39. Elucidating the folding problem of helical peptides using empirical parameters. III. Temperature and pH dependence
40. The adenovirus E4orf6 protein can promote E1A/E1B-induced focus formation by interfering with p53 tumor suppressor function
41. Two distinct activities contribute to the oncogenic potential of the adenovirus type 5 E4orf6 protein
42. The adenovirus E4orf6 protein contributes to malignant transformation by antagonizing E1A-induced accumulation of the tumor suppressor protein p53
43. "Hit-and-run" transformation by adenovirus oncogenes
44. Creation and repair of specific DNA double-strand breaks in vivo following infection with adenovirus vectors expressing Saccharomyces cerevisiae HO endonuclease
45. An arginine-faced amphipathic alpha-helix is required for adenovirus type 5 E4orf6 protein function
46. Localization of the adenovirus early region 1B 55-kilodalton protein during lytic infection: Association with nuclear viral inclusions requires the early region 4 34-kilodalton protein
47. Adenovirus late gene expression does not require a Rev-like nuclear RNA export pathway
48. Structural analysis of the adenovirus type 5 E1B 55-kilodalton-E4orf6 protein complex
49. The "megaprimer" method of site-directed mutagenesis
50. A monoclonal antibody detecting the adenovirus type 5-E1b-58Kd tumor antigen: Characterization of the E1b-58Kd tumor antigen in adenovirus-infected and -transformed cells
51. Analyses of single-amino-acid substitution mutants of adenovirus type 5 E1B-55K protein
52. Distinct regulation of p53 and p73 activity by adenovirus E1A, E1B, and E4orf6 proteins
53. RNA binding by the novel helical domain of the influenza virus NS1 protein requires its dimer structure and a small number of specific basic amino acids
54. The nuclear export signal within the E4orf6 protein of adenovirus type 5 supports virus replication and cytoplasmic accumulation of viral mRNA
55. A cell line that supports the growth of a defective early region 4 deletion mutant of human adenovirus type 2
56. Localization of the E1B proteins of adenovirus 5 in transformed cells, as revealed by interaction with monoclonal antibodies
57. Raf-1 kinase and exoenzyme S interact with 14-3-3ζ through a common site involving lysine 49