RNA binding by the novel helical domain of the influenza virus NS1 protein requires its dimer structure and a small number of specific basic amino acids

RNA

Volumn 5, Issue 2, 1999, Pages 195-205

  Wang, Weirong ^a   Riedel, Kelly ^a   Lynch, Patricia ^a   Chien, Chen Ya ^a,b   Montelione, Gaetano T ^a,b   Krug, Robert M ^a  

^a RUTGERS UNIVERSITY   (United States)

^b RUTGERS UNIVERSITY   (United States)

Author keywords

Influenza virus NS1 protein; Protein dimerization; RNA binding epitope; RNA binding domain

Indexed keywords

ALANINE; AMINO ACID; ARGININE; DIMER; DOUBLE STRANDED RNA; EPITOPE; LYSINE; RNA; RNA BINDING PROTEIN; SMALL NUCLEAR RNA; VIRUS PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DIMERIZATION; INFLUENZA VIRUS A; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN RNA BINDING;

AMINO ACID SEQUENCE; BINDING SITES; DIMERIZATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; POINT MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS; RNA, DOUBLE-STRANDED; RNA-BINDING PROTEINS; SEQUENCE ALIGNMENT; VIRAL NONSTRUCTURAL PROTEINS;

INFLUENZA A VIRUS; INFLUENZA VIRUS;

EID: 0032995665     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838299981621     Document Type: Article

References (30)

1. Alonso-Caplen FV, Nemeroff ME, Yun Q, Krug RM. 1992. Nucleo-cytoplasmic transport: The influenza virus NS1 protein regulates the transport of spliced NS2 mRNA and its precursor NS1 mRNA. Genes & Dev 6:255-267.
2. Banner DW, Kokkinidis M, Tsernoglou D. 1987. Structure of the ColE1 rop protein at 1.7 Å resolution. J Mol Biol 196:657-675.
3. Battiste J, Mao H, Rao N, Tan R, Muhandiram D, Kay L, Frankel A, Williamson J. 1996. Alpha helix-RNA major groove recognition in an HIV-1 rev peptide-RRE RNA complex. Science 273:1547-1551.
4. Berglund H, Rak A, Serganov A, Garber M, Hard T. 1997. Solution structure of the ribosomal RNA binding protein S15 from Thermus thermophilus. Nature Struct Biol 4:20-23.
5. Briedis D, Lamb RA. 1982. Influenza B virus genome: Sequences and structural organization of RNA segment 8 and the mRNAs coding for the NS1 and NS2 proteins. J Virol 42:186-193.
6. Bycroft M, Grunert S, Murzin AG, Proctor M, St Johnston D. 1995. NMR solution structure of a dsRNA binding domain from Drosophila staufen protein reveals homology to the N-terminal domain of ribosomal protein S5. EMBO J 14:3563-3571.
7. Chien C-Y, Tejero R, Huang Y, Zimmerman DE, Krug RM, Montelione GT. 1997. A novel RNA binding motif in influenza A virus non-structural protein 1. Nature Struct Biol 4:891-895.
8. Davies C, Gerstner RB, Draper DE, Ramakrishnan V, White SW. 1998. The crystal structure of ribosomal protein S4 reveals a two-domain molecule with an extensive RNA-binding surface: One domain shows homology to the ETS DNA-binding motif. EMBO J 17:4545-4558.
9. Finerty PJ Jr, Bass BL. 1997. A Xenopus zinc finger protein that specifically binds dsRNA and RNA-DNA hybrids. J Mol Biol 27:195-208.
10. Fortes P, Beloso A, Ortin J. 1994. Influenza virus NS1 protein inhibits pre-mRNA splicing and blocks mRNA nucleocytoplasmic transport. EMBO J 13:704-712.
11. Hatada E, Futada R. 1992. Binding of influenza A virus NS1 protein to dsRNA in vitro. J Gen Virol 73:3325-3329.
12. Hinck AP, Markus MA, Huang S, Grzesiek S, Kustonovich I, Draper DE, Torchia DA. 1997. The RNA binding domain of ribosomal protein L11: Three-dimensional structure of the RNA-bound form of the protein and its interaction with 23 S rRNA. J Mol Biol 274:101-113.
13. Kharrat A, Macias MJ, Gibson TJ, Nilges M, Pastore A. 1995. Structure of the dsRNA binding domain of E. coli RNase III. EMBO J 14:3572-3584.
14. Liu J, Lynch PA, Chien C-Y, Montelione GT, Krug RM, Berman HM. 1997. Crystal structure of the unique RNA-binding domain of the influenza virus NS1 protein. Nature Struct Biol 4:896-899.
15. Lu Y, Qian X, Krug RM. 1994. The influenza virus NS1 protein: A novel inhibitor of pre-mRNA splicing. Genes & Dev 8:1817-1828.
16. Lu Y Wambach M, Katze MG, Krug RM. 1995. Binding of the influenza virus NS1 protein to double-stranded RNA inhibits the activation of the protein kinase that phosphorylates the elF-2 translation initiation factor. Virology 214:222-228.
17. Markus MA, Gerstner RB, Draper DE, Torchia DA. 1998. The solution structure of ribosomal protein S4 Δ41 reveals two subdomains and a positively charged surface that may interact with RNA. EMBO J 17:4559-4571.
18. Nagai K. 1996. RNA-protein complexes. Curr Opin Struct Biol 6:53-61.
19. Nemeroff ME, Barabino SM, Li Y, Keller W, Krug RM. 1998. Influenza virus NS1 protein interacts with the cellular 30K subunit of CPSF and inhibits 3′ end formation of cellular pre-mRNAs. Mol Cell 7:991-1000.
20. Nemeroff ME, Qian X, Krug RM. 1995. The influenza virus NS1 protein forms multimers in vitro and in vivo. Virology 212:422-428.
21. Qian X, Alonso-Caplen F, Krug RM. 1994. Two functional domains of the influenza virus NS1 protein are required for regulation of nuclear export of mRNA. J Virol 68:2433-2441.
22. Qian X, Chien C, Lu Y, Montelione GT, Krug RM. 1995. An amino-terminal polypeptide fragment of the influenza virus NS1 protein possesses specific RNA binding activity and largely helical backbone structure. RNA 1:948-956.
23. Qiu Y, Krug RM. 1994. The influenza virus NS1 protein is a poly(A)-binding protein that inhibits nuclear export of mRNAs containing poly(A). J Virol 68:2425-2432.
24. Qiu Y, Nemeroff ME, Krug RM. 1995. The influenza virus NS1 protein binds to a specific region in human U6 snRNA and inhibits U6-U2 and U6-U4 snRNA interactions during splicing. RNA 1:304-316.
25. Stebbins CE, Borukhov S, Orlova M, Polyakov A, Goldfarb A, Darst SA. 1995. Crystal structure of the GreA transcript cleavage factor from Escherichia coli. Nature 373:636-640.
26. Stewart L, Redinbo MR, Qiu X, Hol WG, Champoux JJ. 1998. A model for the mechanism of human topoisomerase I. Science 279:1534-1541.
27. Wang W, Krug RM. 1996. The RNA-binding and effector domains are conserved to different extents among influenza A and B viruses. Virology 223:41-50.
28. Wang W, Krug RM. 1998. U6atac snRNA, the highly divergent counterpart of U6 snRNA, is the specific target that mediates inhibition of AT-AC splicing by the influenza virus NS1 protein. RNA 4:55-64.
29. Weeks KM, Crothers DM. 1993. Major groove accessibility of RNA. Science 261:1574-1577.
30. Xing Y, GuhaThakurta D, Draper DE. 1997. The RNA binding domain of ribosomal protein L11 is structurally similar to homeodomains. Nature Struc Biol 4:24-27.