Lens proteomics: The accumulation of crystallin modifications in the mouse lens with age

Investigative Ophthalmology and Visual Science

Volumn 43, Issue 1, 2002, Pages 205-215

  Ueda, Y ^a   Duncan, M K ^a   David, L L ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA CRYSTALLIN; BETA CRYSTALLIN; CRYSTALLIN; GAMMA CRYSTALLIN; LENS PROTEIN; PEPTIDE; PROTEOME;

ACIDITY; AGING; AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; CHROMATOGRAPHY; IMAGE ANALYSIS; ISOELECTRIC POINT; LENS; MASS SPECTROMETRY; MOUSE; MOUSE STRAIN; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; PROTEIN POLYMORPHISM; PROTEIN PROCESSING; SOLUBILITY; TWO DIMENSIONAL ELECTROPHORESIS;

AGING; ANIMALS; CHROMATOGRAPHY, GEL; CRYSTALLINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; LENS, CRYSTALLINE; MICE; MICE, INBRED C57BL; MICE, INBRED ICR; MICE, KNOCKOUT; MICE, TRANSGENIC; PHOSPHORYLATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEOME;

EID: 0036139647     PISSN: 01460404     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (53)

1. Regional differences in protein synthesis within the lens of the rat
2. Cataract and the acceleration of calpain-induced beta-crystallin insolubilization occurring during normal maturation of rat lens
3. Age-related changes in human lens crystallins identified by two-dimensional electrophoresis and mass spectrometry
4. Truncation of betaA3/A1-crystallin during aging of the bovine lens: Possible implications for lens optical quality
5. Targeted disruption of the mouse alpha A-crystallin gene induces cataract and cytoplasmic inclusion bodies containing the small heat shock protein alpha B-crystallin
6. Mice deficient for the secreted glycoprotein SPARC/osteonectin/BM40 develop normally but show severe age-onset cataract formation and disruption of the lens
7. Genetic factors influence cataract formation in alpha 3 connexin knockout mice
8. Disruption of lens fiber cell differentiation and survival at multiple stages by region-specific expression of truncated FGF receptors
9. Over-expression of PAX6(5a) in lens fiber cells results in cataract and upregulation of (alpha)5(beta)1 integrin expression
10. DeltaFosB-induced cataract
11. Cysteine protease activated by expression of HIV-1 protease in transgenic mice. MIP26 (aquaporin-0) cleavage and cataract formation in vivo and ex vivo
12. Transgenic mice: Models for the study of cataractogenesis: A minireview
13. Mouse models of congenital cataract
14. Characterization of abnormal proteins in the soluble lens proteins of CatFraser mice
15. Modifications in lens protein biosynthesis signal the initiation of cataracts induced by buthionine sulfoximine in mice
16. Identification of mouse crystallins in 2D protein patterns by sequencing and mass spectrometry: Application to cataract mutants
17. FVB/N: An inbred mouse strain preferable for transgenic analyses
18. Genealogies of mouse inbred strains
19. Formation of corneal endothelium is essential for anterior segment development: A transgenic mouse model of anterior segment dysgenesis
20. Regulation of the murine alpha A-crystallin promoter in transgenic mice
21. Lens proteomics: Analysis of rat crystallin sequences and two-dimensional electrophoresis map
22. Rat lens gamma-crystallins: Characterization of the six gene products and their spatial and temporal distribution resulting from differential synthesis
23. Identification of proteins by matrixassisted laser desorption/ionization mass spectrometry using peptide and fragment ion masses
24. Staining of preparative 2-D gels: Coomassie blue and imidazole-zinc negative staining
25. A procedure for protein clution from reverie-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
26. Lens epithelial cell mRNA, II: Expression of a mRNA encoding a lipid-binding protein in rat lens epithelial cells
27. LP2, a differentiation-associated lipid-binding protein expressed in bovine lens
28. Alternative RNA splicing of the murine alpha A-crystallin gene: Protein-coding information within an intron
29. Lop12, a mutation in mouse Crygd causing lens opacity similar to human Coppock cataract
30. Three murine cataract mutants (Cat2) are defective in different gamma-crystallin genes
31. Differential expression of alpha A-and alpha B-crystallin during murine ocular development
32. Crystallin gene expression during rat lens development
33. Immunocytochemical localization of the 27K beta-crystallin polypeptide in the mouse lens during development using a specific monoclonal antibody: Implications for cataract formation in the Philly mouse
34. Age-related changes in human lens crystallins identified by HPLC and mass spectrometry
35. Differential synthesis of crystallins in the developing rat eye lens
36. Cloning and mapping the mouse Crygs gene and non-lens expression of [gamma] S-crystallin
37. Temporal regulation of six crystallin transcripts during mouse lens development
38. Differential phosphorylation of alpha-A crystallin in human lens of different age
39. Post-translational modifications of water-soluble human lens crystallins from young adults
40. Age-related changes in bovine alpha-crystallin and high-molecular-weight protein
41. The effect of stress on the pattern of phospliorylation of alphaA and alphaB crystallin in the rat lens
42. Phosphorylations of alpha A- and alpha B-crystallin
43. Phosphorylation-induced change of the oligomerization state of alphaB-crystallin
44. Structure and modifications of the junior chaperone alpha-crystallin: From lens transparency to molecular pathology
45. The C-terminal region of alpha-crystallin: Involvemeni in protection against heat-induced denaturation
46. Alpha-Crystallin chaperone activity is reduced by calpain II in vitro and in selenite cataract
47. Probing the structure and interactions of crystallin proteins by NMR spectroscopy
48. Lp82 is the dominant form of calpain in young mouse lens
49. Modifications of the water-insoluble human lens alpha-crystallins
50. Identification of the in vivo truncation sites at the C-terminal region of alpha-A crystallin from aged bovine and human lens
51. Human-mouse differences in the embryonic expression patterns of developmental control genes and disease genes
52. Buthionine sulfoximine induced cataracts in mice contain insolubilized crystallins with calpain II cleavage sites