Development of enzymatic assays for quantification of intracellular lamivudine and carbovir triphosphate levels in peripheral blood mononuclear cells from human immunodeficiency virus-infected patients

Antimicrobial Agents and Chemotherapy

Volumn 46, Issue 1, 2002, Pages 135-143

  Kewn, S ^a   Hoggard, P G ^a   Sales, S D ^a   Jones, K ^a   Maher, B ^a   Khoo, S H ^a   Back, D J ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOVIR; CELL EXTRACT; DRUG METABOLITE; LAMIVUDINE; NUCLEOSIDE TRIPHOSPHATE; RNA DIRECTED DNA POLYMERASE;

ARTICLE; CELL LEVEL; CONTROLLED STUDY; DNA TEMPLATE; DOSE RESPONSE; DRUG HALF LIFE; ENZYME ASSAY; ENZYME INHIBITION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; INCUBATION TIME; ISOTOPE LABELING; MONONUCLEAR CELL; NONHUMAN; PRIORITY JOURNAL;

CHEMISTRY, ANALYTICAL; DEOXYGUANINE NUCLEOTIDES; HIV INFECTIONS; HUMANS; LAMIVUDINE; QUALITY CONTROL; REFERENCE STANDARDS;

EID: 0036136728     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.46.1.135-143.2002     Document Type: Article

References (22)

1. Zidovudine phosphorylation in HIV-infected patients and seronegative volunteers
2. The effect of zidovudine dose on the formation of intracellular phosphorylated metabolites
3. Cellular metabolism of (-) enantiomeric 2′-deoxy-3′-thiacytidine
4. Human immunodeficiency virus reverse transcriptase: General properties and its interaction with nucleoside triphosphate analogs
5. Comparitive kinetic analyses of interaction of inhibitors with rauscher murine leukemia virus and human immunodeficiency virus reverse transcriptase
6. 1592U89, a novel carbocyclic nucleoside analog with potent selective anti-human immunodeficiency virus activity
7. The intracellular phosphorylation of (-)-2′-deoxy-3′-thiacytidine (3TC) and the incorporation of 3TC 5′-monophosphate into DNA by HIV-1 reverse transcriptase and human DNA polymerase γ
8. Zidovudine phosphorylation determined sequentially over 12 months in human immunodeficiency virus-infected patients with or without previous exposure to antiretroviral agents
9. Lamivudine (3TC) phosphorylation and drug interactions in vitro
10. Simultaneous quantitation of the 5′-triphosphate metabolites of zidovudine, lamivudine, and stavudine in peripheral mononuclear blood cells of HIV infected patients by high-performance liquid chromatography tandem mass spectrometry
11. The pharmacokinetics of lamivudine phosphorylation in peripheral blood mononuclear cells from patients infected with HIV-1
12. Enzymatic assay for measurement of zidovudine triphosphate in peripheral blood mononuclear cells
13. Development of a new cartridge radioimmunoassay for determination of intracellular levels of lamivudine triphosphate in the peripheral blood mononuclear cells of human immunodeficiency virus-infected patients
14. A systemic and cellular model for zidovudine plasma concentrations and intracellular phosphorylation in patients
15. Simultaneous quantitation of intracellular zidovudine and lamivudine triphosphates in human immunodeficiency virus-infected individuals
16. Enzymatic assay for deoxynucleoside triphosphates using synthetic oligonucleotides as template primers
17. Intracellular zidovudine (ZDV) and ZDV phosphates as measured by a validated combined high-pressure liquid chromatography-radioimmunoassay procedure
18. A coupled HPLC/radioimmunoassay for analysis of zidovudine metabolites in mononuclear cells
19. Comparative enzymatic study of HIV-1 reverse transcriptase resistant to 2′,3′-dideoxynucleotide analogues using the single-nucleotide incorporation assay
20. Enzymatic characterization of human immunodeficiency virus type 1 reverse transcriptase resistant to multiple 2′,3′-dideoxynucleoside-5′-triphosphates