Characterization of a Legionella pneumophila relA insertion mutant and roles of RelA and RpoS in virulence gene expression

Journal of Bacteriology

Volumn 184, Issue 1, 2002, Pages 67-75

  Zusman, T ^a   Gal Mor, O ^a   Segal, G ^a  

^a TEL AVIV UNIVERSITY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; SIGMA FACTOR RPOS; TRANSCRIPTION FACTOR RELA; UNCLASSIFIED DRUG;

ACANTHAMOEBA CASTELLANII; ARTICLE; BACTERIAL GROWTH; BACTERIAL VIRULENCE; CELL GROWTH; CONTROLLED STUDY; FLAGELLUM; GENE CONTROL; GENE DELETION; GENE EXPRESSION; GENE FUSION; GENE INSERTION; GENE MUTATION; LEGIONELLA PNEUMOPHILA; MACROPHAGE; NONHUMAN; PLASMID; PRIORITY JOURNAL;

ACANTHAMOEBA; ANIMALS; BACTERIAL PROTEINS; EUKARYOTIC CELLS; FLAGELLIN; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENES, REPORTER; GUANOSINE TETRAPHOSPHATE; HL-60 CELLS; HUMANS; LEGIONELLA PNEUMOPHILA; LIGASES; MACROPHAGES; MUTAGENESIS, INSERTIONAL; PIGMENTS, BIOLOGICAL; SIGMA FACTOR;

ACANTHAMOEBA; ACANTHAMOEBA CASTELLANII; BACTERIA (MICROORGANISMS); INSERTION SEQUENCES; LEGIONELLA; LEGIONELLA PNEUMOPHILA; NEGIBACTERIA; PROTOZOA;

EID: 0036136235     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.1.67-75.2002     Document Type: Article

References (53)

1. The phagosome containing Legionella pneumophila within the protozoan Hartmannella vermiformis is surrounded by the rough endoplasmic reticulum
2. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
3. Identification of linked Legionella pneumophila genes essential for intracellular growth and evasion of the endocytic pathway
4. RpoS co-operates with other factors to induce Legionella pneumophila virulence in the stationary phase
5. Altered intracellular targeting properties associated with mutations in the Legionella dotA gene
6. Interaction of Legionella pneumophila with Acanthamoeba castellanii: Uptake by coiling phagocytosis and inhibition of phagosomesosome fusion
7. The Legionella pneumophila icm locus: A set of genes required for intracellular multiplication in human macrophages
8. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals
9. Detection of (p)ppGpp accumulation patterns in Escherichia coli mutants
10. The stringent response
11. Characterization of the selective inhibition of growth of virulent Legionella pneumophila by supplemented Mueller-Hinton medium
12. The ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) plays a conditional role in antibiotic production and morphological differentiation
13. Discovery of virulence genes of Legionella pneumophila by using signature tagged mutagenesis in a guinea pig pneumonia model
14. The molecular ecology of legionellae
15. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd
16. Synthesis of the stationary-phase sigma factor sigma S is positively regulated by ppGpp
17. The Legionella pneumophila rpoS gene is required for growth within Acanthamoeba castellanii
18. Co-ordination of Legionella pneumophila virulence with entry into stationary phase by ppGpp
19. The guanosine nucleotide (p)ppGpp initiates development and A-factor production in Myxococcus xanthus
20. DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae
21. The alternative sigma factor sigma-28 of Legionella pneumophila restores flagellation and motility to an Escherichia coli fliA mutant
22. Characterization of avirulent mutant Legionella pneumophila that survive but do not multiply within human monocytes
23. Formation of a novel phagosome by the Legionnaires' disease bacterium (Legionella pneumophila) in human monocytes
24. The Legionnaires' disease bacterium (Legionella pneumophila) inhibits phagosome-lysosome fusion in human monocytes
25. Phagocytosis of the Legionnaires' disease bacterium (Legionella pneumophila) occurs by a novel mechanism: Engulfment within a pseudopod coil
26. Legionella pneumophila inhibits acidification of its phagosome in human monocytes
27. Legionnaires' disease bacterium (Legionella pneumophila) multiplies intracellularly in human monocytes
28. The transfer region of IncIplasmid R64: Similarities between R64 tra and Legionella icm/dot genes
29. Identification of transcriptional start sites and the role of ppGpp in the expression of rpoS, the structural gene for the sigma S subunit of RNA polymerase in Escherichia coli
30. Identification of a Legionella pneumophila locus required for intracellular multiplication in human macrophages
31. The Legionella pneumophila icmGCDJBF genes are required for killing of human macrophages
32. Current views on the relationships between amoebae, legionellae and man
33. Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae
34. Identification of Legionella pneumophila genes required for growth within and killing of human macrophages
35. The iron superoxide dismutase of Legionella pneumophila is essential for viability
36. Host cell killing and bacterial conjugation require overlapping sets of genes within a 22-kb region of the Legionella pneumophila genome
37. Characterization of a new region required for macrophage killing by Legionella pneumophila
38. How is the intracellular fate of the Legionella pneumophila phagosome determined?
39. Intracellular multiplication and human macrophage killing by Legionella pneumophila are inhibited by conjugal components on IncQ plasmid RSF1010
40. Legionella pneumophila utilizes the same genes to multiply within Acanthamoeba castellanii and human macrophages
41. Possible origin of the Legionella pneumophila virulence genes and their relation to Coxiella burnetii
42. Intracellular growth of Legionella pneumophila in Dictyostelium discoideum, a system for genetic analysis of host-pathogen interactions
43. The Lly protein protects Legionella pneumophila from light but does not directly influence its intracellular survival in Hartmannella vermiformis
44. Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen
45. Association of Legionella pneumophila with the macrophage endoplasmic reticutum
46. Conjugative transfer by the virulence system of Legionella pneumophila
47. Cell biology of Legionella pneumophila
48. Early events in phagosome establishment are required for intracellular survivel of Legionella pneumophila
49. Mutagenesis of Legionella pneumophila using Tn903dilacZ: Identification of a growth-phase-regulated pigmentation gene
50. Sequence determination and mutational analysis of the y locus of Legionella pneumophila
51. Residual guanosine 3′,5′-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spot null mutations
52. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors