The alternative sigma factor σ28 of Legionella pneumophila restores flagellation and motility to an Escherichia coli fliA mutant

Journal of Bacteriology

Volumn 179, Issue 1, 1997, Pages 17-23

  Heuner, Klaus ^a   Hacker, Jörg ^a   Brand, Bettina C ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

SIGMA FACTOR;

AMINO ACID SEQUENCE; ARTICLE; BACTERIUM MUTANT; CELL MOTILITY; ESCHERICHIA COLI; FLAGELLUM; LEGIONELLA PNEUMOPHILA; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PSEUDOMONAS AERUGINOSA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA; ESCHERICHIA COLI; ESCHERICHIA COLI K12; LEGIONELLA PNEUMOPHILA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0031029457     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.1.17-23.1997     Document Type: Article

References (49)

1. Allison, C., N. Coleman, P. L. Jones, and C. Hughes. 1992. Ability of Proteus mirabilis to invade human urothelial cells is coupled to motility and swarming differentiation. Infect. Immun. 60:4740-4740.
2. Arnosti, D. N., and M. J. Chamberlin. 1989. Secondary sigma factor controls transcription of flagellar and chemotaxis genes in Escherichia coli. Proc. Natl. Acad. Sci, USA 86:830-834.
3. Berger K. H., and R. R. Isberg. 1993. Two distinct defects in intracellular growth complemented by a single genetic locus in Legionella pneumophila. Mol. Microbiol. 7:7-19.
4. Berger, K. H., J. J. Merriam, and R. R. Isberg. 1994. Altered intracellular targeting properties associated with mutations in the Legionella pneumophila dotA gene, Mol. Microbiol. 14:809-822.
5. Brand, B. C., A. B. Sadosky, and H. A. Shuman. 1994. The Legionella pneumophila icm locus: a sel of genes required for intracellular multiplication in human macrophages. Mol. Microbiol. 14:797-808.
6. Brand, B. C., and J. Hacker. 1996. The biology of Legionella infection. In S. H. E. Kaufmann (ed.), Host response to intracellular pathogens, p. 291-312, R. G. Landes Company.
7. Chater, K. F., C. J. Bruton, K. A. Plaskitt, M. J. Buttner, C. Méndez, and J. D. Helmann. 1989. The developmeniul fate of S. coelicolor hyphae depends upon a gene product homologous with the motility sigma factor of B. subtilis. Cell 59:133-143.
8. Chen, Y.-F., and J. D. Helmann. 1992. Restoration of motility to an Excherichia coli fliA flagellar mutant by a Bacillus subtilis σ-factor. Proc. Natl. Acad. Sci. USA 89:5123-5127.
9. Cianciotto, N. P., and B. S. Fields. 1992. Legionella pneumophila mip gene potentiates intracellular infection of protozoa and human macrophages. Proc. Natl. Acad. Sci. USA 89:5188-5191.
10. Cianciotto, N. P., B. I. Eisenstein, C. H. Mody, G. B. Toews, and N. C. Engleberg. 1989. A Legionella pneumophila gene encoding a species-specific surface protein potentiates initiation of intracellular infection. Infect. Immun. 57:1255-1262.
11. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
12. Drake, D., und T. C. Montie. 1988. Flagella, motility. and invasive virulence of Pseudomonas aeruginosa. J. Gen. Microbiol. 134:43-52.
13. Elliot, J. A., and W. Johnson. 1982. Virulence conversion of Legionella pneumophila serogroup 1 by passage in guinea pigs and embryonated eggs. Infect. Immun. 35:943-947.
14. Fields, B. S. 1993. Legionella and protozoa: interaction of a pathogen and its natural host, p. 70-72. In J. M. Barbaree, R. F. Breiman, and A. P. Dufour (ed.), Legionella: current status and emerging perspectives. American Society for Microbiology, Washington, D.C.
15. Gabay, J. E., M. Blake, W. D. Niks, and M. A. Horwitz. 1985. Purification of Legionella pneumophila major outer membrane protein and demonstration that it is a porin. J. Bacteriol. 162:85-91.
16. Gardel, C. L., and J. J. Mekalanos. 1996. Alterations in Vibrio cholerae motility phenotypos correlate with changes in virulence factor expression. Infect. Immun. 64:2246-2255.
17. Gillen, K. L., and K. T. Hughes. 1991. Negative regulatory loci coupling Hagellin synthesis to flagcllar assembly in Salmonella typhimurium. J. Bacteriol. 173:2301-2310.
18. Helmann, J. D. 1991. Alternative sigma factors and the regulation of flagellar gene expression. Mol. Microbiol. 5:2875-2882.
19. Helmann, J. D., and M. J. Chamberlin. 1987, DNA sequence analysis suggests that expression of flagellar and chemotaxis genes in Escherichia coli and Salmonella typhimurium is controlled by an alternative sigma factor. Proc. Natl. Acad. Sci. USA 84:6422-6424.
20. Helmann, J. D., and M. J. Chamberlin. 1988. Structure and function of bacterial sigma factors. Annu. Rev. Biochem. 57:839-872.
21. Heuner, K., L. Bender-Beck, B. C. Brand, P. C. Lück, K.-H. Mann, R. Marre, M. Ott, and J. Hacker. 1995. Cloning and genetic characterization of the flagellum subunit gene (flaA) of Legionella pneumophila serogroup 1. Infect. Immun. 63:2499-2507.
22. Hoffman, P. S., M. Ripley, and K. Weeratna. 1992. Cloning and nucleotide sequence of a gene (ompS) encoding the major outer membrane protein of Legionella pneumophila. J. Bacteriol. 174:914-920.
23. Jepras, R. I., R. B. Fitzgeorge, and A. Baskerville. 1985. A comparison of virulence of two strains of Legionella pneumophila based on experimental aerosol infection of guinea-pigs. J. Hyg. 95:29-38.
24. Komeda, Y., K. Kutsukake, and T. Iino. 1980. Definition of additional flagellar genes in Escherichia coli K12. Genetics 94:277-290.
25. Kutsukake, K., and T. Iino. 1994. Role of the FliA-FlgM regulatory system on the transcriptional control of the flagellar regulon and flagellar formation in Salmonella typhimurium. J. Bacteriol. 176:3598-3605.
26. Kutsukake, K., S. Iyoda, K. Ohnishi, and T. Iino. 1994. Genetic and molecular analyses of the interaction between the flagellum-specific sigma and anti-sigma factors in Salmonella typhimurium. EMBO J. 13:4568-4576.
27. Liu, S.-L., T. Ezaki, H. Miuru, und E. Vabuuchi. 1988. Intact motility as a Salmonella typhi invasion-related factor. Infect. Immun. 56:1967-1973.
28. Lorenzo, L. A., M. Herbro, U. Jakubzik, and K. N. Timmis. 1990. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria. J. Bacteriol. 172:6568-6572.
29. D-dependent functions in Bacillus subtilis. J. Bacteriol. 172:3435-3443.
30. Marra, A., S. J. Blander, M. A. Horwitz, and H. A. Shuman. 1992. Identification of a Legionella pneumophila locus required for intracellulur multiplication in human macrophages. Proc. Natl. Acad. Sci. USA 89:9607-9611.
31. Méndez, C., and K. F. Chater. 1987. Cloning of whiG, a gene critical for sporulation of Streptomyces coelicolor A3(2). J. Bacteriol. 169:5715-5720.
32. Mirel, D. B., and M. J. Chamberlin. 1989. The Bacillus subtilis flagellin gene (hag) is transcribed by the sigma 28 form of RNA polymerase. J. Bacteriol. 171:3095-3101.
33. Morales, V. M., A. Baeckmann, and M. Bagdasarian. 1991. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97:39-47.
34. Mytelka, D. S., and M. J. Chamberlin. 1996. Escherichta cali fliAZV operon. J. Bacteriol. 178:24-34.
35. Ohnishi, K., K. Kutsukake, H. Suzuki, and T. Iino. 1990. Gene fliA encodes an alternative sigma factor specific for flagellar operons in Salmonella typhimurium, Mol. Gen. Genet. 221:139-147.
36. Payne N. R., and M. A. Horwitz. 1987. Phagocytosis of Legionella pneumophila is mediated by human monocyte complement receptors. J. Exp. Med. 166:1377-1389.
37. Pearson, W. R., and D. J. Lipman. 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85:2444-2448.
38. Truckler, J. M., R. F. Benson, M. Moyenuddin, W. T. Martin, and B. S. Fields. 1995. Association of flagellum expression and intracellular growth of Legionella pneumophila. Infect. Immun. 63:4928-4932.
39. Rowbotham, T. J. 1980. Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and sou amoebae. J. Clin. Pathol. 33:1179-1183.
40. Rowbotham, T. J. 1986. Current view on the relationship between amoebae, legionellae and man. Isr. J. Med. Sci. 22:678-689.
41. Saiki, R. K., D. H. Gelfand, S. Stoffel, S. J. Scharf, R. Higucni, G. T. Horn, K. H. Mullis, and H. A. Erlich. 1988. Primer-directed enzymatic amplification of DNA with thermostable DNA-polymerase. Science 239:487-491.
42. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
43. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
44. Schmitt, C. K., S. C. Darnell, V. L. Tesh, B. A. Stocker, and A. D. O'Brien. 1994. Mutation of flgM attenuates virulence of Salmonella typhimurium, and mutation of fliA represses the attenuated phenotype. J. Bacteriol. 176:368-377.
45. Starnbach, M. N., and S. Lory. 1992. The fli1 (rpoF) gene of Pseudomonas aeruginosa encodes an alternative sigma factor required for flagellin synthesis. Mol. Microbiol. 6:459-469.
46. Steinert, M., M. Ott, P. C. Lück, and J. Hacker. 1994. Studies on the uptake and intracellular replication of Legionella pneumophila in protozoa and in macrophage-like cells, FEMS Microbiol. Ecol. 15:299-308.
47. Wintermeyer, E., B. Ludwig, M. Steinert, B. Schmidt, G. Fischer, and J. Hacker. 1995. Influence of site specifically altered Mip proteins on intracellular survival of Legionella pneumophila in eukaryotic cells. Infect. Immun. 63:4576-4583.
48. Woodcock, D. M., P. J. Crowther, J. Doherty, S. Jefferson, E. DeCruz, M. Nayer-Weidner, S. S. Smith, M. Z. Michael, and M. W. Graham. 1989. Quantitation evaluation of Escherichia coli for tolerance of cytosine methylation in plasmid and phage recombination. Nucleic Acids Res. 9:3469-3478.
49. Yao, R., D. H. Burr, P. Doig, T. J. Trust, H. Niu, and P. Guerry. 1994. Isolation of motile and non-motile insertional mutants of Campylobacter jejuni: the role of motility in adherence and invasion of eukaryotic cells. Mol. Microbiol. 14:883-893.