Identification of linked Legionella pneumophila genes essential for intracellular growth and evasion of the endocytic pathway

Infection and Immunity

Volumn 66, Issue 3, 1998, Pages 950-958

  Andrews, Helene L ^b,c   Vogel, Joseph P ^b   Isberg, Ralph R ^a,b  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c School of Veterinary Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIUM MUTANT; ENDOSOME; GENE ISOLATION; GENE MUTATION; LEGIONELLA PNEUMOPHILA; LEGIONNAIRE DISEASE; MOUSE; NONHUMAN; OPEN READING FRAME; PHAGOSOME; PRIORITY JOURNAL;

EID: 0031937678     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.66.3.950-958.1998     Document Type: Article

References (50)

1. Andrews, H. L., J. P. Vogel, and R. R. Isberg. Unpublished data.
2. Berger, K. H., and R. R. Isberg. 1993. Two distinct defects in intracellular growth complemented by a single genetic locus in Legionella pneumophila. Mol. Microbiol. 7:7-19.
3. Berger, K. H., J. J. Merriam, and R. R. Isberg. 1994. Altered intracellular targeting properties associated with mutations in the Legionella pneumophila dotA gene. Mol. Microbiol. 14:804-822.
4. Brand, B. C., A. B. Sadosky, H. A. Shuman. 1994. The Legionella pneumophila icm locus: a set of genes required for intracellular multiplication in human macrophages. Mol. Microbiol. 14:797-808.
5. Bullock, W. O., J. M. Fernandez, and J. M. Short. 1987. XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. BioTechniques 5:376-378.
6. Catrenich, C. E., and W. Johnson. 1484. Characterization of the selective inhibition of growth of virulent Legionella pneumophila by supplemented Mueller-Hinton medium. Infect. Immun. 57:1862-1864.
7. Censini, S., C. Lange, Z. Xiang, J. E. Crabtree, P. Ghiara, M. Borodovski, R. Rappuoli, and A. Covacci. 1996. cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease associated virulence factors. Proc. Natl. Acad. Sci. USA 93:14648-14653.
8. Chen, J. W., Y. Cha, K. U. Yuksel, R. W. Gracy, and J. T. August. 1988. Isolation and sequencing of a cDNA clone encoding lysosomal membrane glycoprotein mouse LAMP-1. J. Biol. Chem. 263:8754-8758.
9. Cianciotto, N., B. I. Eisenstein, and N. C. Engleberg. 1989. Genetics and molecular pathogenesis of Legionella pneumophila, an intracellular parasite of macrophages. Mol. Biol. Med. 6:409-424.
10. Clemens, D. L., and M. A. Horwitz. 1995. Characterization of the Mycobacterium tuberculosis phagosome and evidence that phagosomal maturation is inhibited. J. Exp. Med. 181:257-270.
11. Del Sal, G., G. Manfioletti, and C. Schneider. 1989. The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages, or plasmids suitable for sequencing. BioTechniques 7:514-519.
12. Feeley, J. C., R. J. Gibson, G. W. Gorman, N. C. Langford, J. K. Rasheed, D. C. Makel, and W. B. Blaine. 1979. Charcoal yeast extract agar: primary isolation medium for Legionella pneumophila. J. Clin. Microbiol. 10:437-441.
13. Fields, B. S., G. N. Sanden, J. M. Barbaree, W. E. Morrill, R. M. Wadowsky, E. H. White, and J. C. Feeley. 1989. Intracellular multiplication of Legionella pneumophila in amoebae isolated from hospital hot water tanks. Curr. Microbiol. 18:131-137.
14. Finan, T. M., B. Kunkel, G. De Vos, and E. R. Signer. 1986. Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes. J. Bacteriol. 167:66-72.
15. Gabay, J. E., and M. A. Horwitz. 1985. Isolation and characterization of the cytoplasmic and outer membranes of the Legionnaires' disease bacterium (Legionella pneumophila). J. Exp. Med. 161:409-422.
16. Hobbs, M., and J. S. Mattick. 1993. Common components in the assembly of type 4 fimbriae, DNA transfer systems, filamentous phage and proteinsecretion apparatus: a general system for the formation of surface-associated protein complexes. Mol. Microbiol. 10:233-243.
17. Horwitz, M. A. 1987. Characterization of avirulent mutant Legionella pneumophila that survive hut do not multiply within human monocytes. J. Exp. Med. 166:1310-1328.
18. Horwitz, M. A. 1983. Formation of a novel phagosome by the Legionnaires' disease bacterium (Legionella pneumophila) in human monocytes. J. Exp. Med. 158:1319-1331.
19. Horwitz, M. A. 1983. The Legionnaires' disease bacterium (Legionella pneumophila) inhibits phagosome-lysosome fusion in human monocytes. J. Exp. Med. 158:2108-2126.
20. Horwitz, M. A. 1984. Phagocytosis of the Legionnaires' disease bacterium (Legionella pneumophila) occurs by a novel mechanism: engulfment within a pseudopod coil. Cell 36:27-33.
21. Horwitz, M. A., and F. R. Maxfield. 1984. Legionella pneumophila inhibits acidification of its phagosome in human monocytes. J. Cell Biol. 99:1936-1943.
22. Husmann, L. K., and W. Johnson. 1994. Cytotoxicity of extracellular Legionella pneumophila. Infect. Immun. 62:2111-2114.
23. Kirby, J. E., J. P. Vogel, H. L. Andrews, and R. R. Isberg. Evidence for pore forming ability by Legionella pneumophila. Mol. Microbiol., in press.
24. Kolter, R., M. Inuzuka, and D. R. Helinski. 1978. Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K. Cell 15:1199-1208.
25. Marra, A., S. J. Blander, M. A. Horwitz, and H. A. Shuman. 1992. Identification of Legionella pneumophila locus required for intracellular multiplication in human macrophages. Proc. Natl. Acad. Sci. USA 89:9607-9611.
26. McLean, I. W., and P. K. Nakane. 1974. Periodate-lysine-paraformaldehyde fixative: a new fixative for immunoelectron microscopy. J. Histochem. Cytochem. 22:1077-1083.
27. Merriam, J. J., R. Mathur, R. Maxfield-Boumil, and R. R. Isberg. 1997. Analysis of the Legionella pneumophila fliI gene: intracellular growth of a defined mutant defective for flagellum biosynthesis. Infect. Immun. 65:2497-2501.
28. Mintz, C. S., J. Chen, and H. A. Shuman. 1998. Isolation and characterization of auxotrophic mutants of Legionella pneumophila that fail to multiply in human monocytes. Infect. Immun. 56:1449-1455.
29. Nash, T. W., D. M. Libby, and M. A. Horwitz. 1984. Interaction between the Legionnaires' disease bacterium (Legionella pneumophila) and human alveolar macrophages. J. Clin. Invest. 74:771-782.
30. Nikaido, H. 1994. Porins and specific diffusion channels in bacterial outer membranes. J. Biol. Chem. 269:3905-3908.
31. Petosa, C., R. J. Collier, K. R. Klimpel, S. H. Leppla, and R. C. Liddington. 1997. Crystal structure of the anthrax toxin protective antigen. Nature 385: 833-838.
32. Roy, C. R., K. H. Berger, and R. R. Isberg. Legionella pneumophila DotA protein is required for early phagosome trafficking decisions that occur within minutes of bacterial uptake. Submitted for publication.
33. Roy, C. R., and R. R. Isberg. 1997. Topology of Legionella pneumophila DotA: an inner membrane protein required for replication in macrophages. Infect. Immun. 65:571-578.
34. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.
35. Sasatsu, M., T. K. Misra, L. Chu, R. Laddaga, and S. Silver. 1985. Cloning and DNA sequence of a plasmid-determined citrate utilization system in Escherichia coli. J. Bacteriol. 164:983-993.
36. Shuman, H. Personal communication.
37. Swanson, M. S. Unpublished data.
38. Swanson, M. S., and R. R. Isberg. 1996. Analysis of intracellular fate of Legionella pneumophila mutants. Ann. N. Y. Acad. Sci. 797:8-18.
39. Swanson, M. S., and R. R. Isberg. 1996. Identification of Legionella pneumophila mutants that have aberrant intracellular fates. Infect. Immun. 64: 2585-2594.
40. Swanson, M. S., and R. R. Isberg. 1995. Association of Legionella pneumophila with the macrophage endoplasmic reticulum. Infect. Immun. 63:3609-3620.
41. Tietze, E., and H. Tschape. 1994. Temperature-dependent expression of conjugation pili by IncM plasmid-harbouring bacteria: identification of plasmid-encoded regulatory functions. J. Basic Microbiol. 34:105-116.
42. Vecsey-Semjen, B., C. Lesieur, R. Moellby, and F. Gisou van der Goot. 1997. Conformational changes due to membrane binding and channel formation by staphylococcal α-toxin. J. Biol. Chem. 272:5709-5717.
43. Vogel, J. P., C. Roy, and R. R. Isberg. 1996. Use of salt to isolate Legionella pneumophila mutants unable to replicate in macrophages. Ann. N. Y. Acad. Sci. 797:271-272.
44. Vogel, J. P., H. L. Andrews, S. K. Wong, and R. R. Isberg. Conjugal transfer by the virulence system of Legionella pneumophila. Science, in press.
45. Vogel, J. P., and R. R. Isberg. Unpublished data.
46. Vogel, J. P., et al. Unpublished data.
47. Wadowsky, R. M., L. J. Butler, C. M. K., S. M. Verma, M. A. Paul, B. S. Fields, G. Keleti, J. L. Sykora, and R. B. Yee. 1988. Growth-supporting activity for Legionella pneumophila in tap water cultures and implication of hartmannellid amoebae as growth factors. Appl. Environ. Microbiol. 54: 2677-2682.
48. Winans, S. C., D. L. Burns, and P. J. Christie. 1996. Adaptation of a conjugal transfer system for the export of pathogenic macromolecules. Trends Microbiol. 6:64-68.
49. Winn, W. C., and R. L. Myerowitz. 1981. The pathology of the Legionella pneumonias. Hum. Pathol. 12:401-422.
50. Woodcock, D. M., P. J. Crowther, J. Doherty, S. Jefferson, E. DeCruz, M. Noyer-Weidner, S. S. Smith, M. Z. Michael, and M. W. Graham. 1989. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 17:3469-3478.