The Legionella pneumophila icmGCDJBF genes are required for killing of human macrophages

Infection and Immunity

Volumn 66, Issue 5, 1998, Pages 2245-2255

  Purcell, Mary ^a   Shuman, Howard A ^a  

^a Columbia University ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL MUTATION; CYTOTOXICITY; DNA HYBRIDIZATION; GENE INSERTION; GENE LOCUS; HUMAN; HUMAN CELL; LEGIONELLA PNEUMOPHILA; MACROPHAGE MIGRATION INHIBITION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; TRANSPOSON;

LEGIONELLA PNEUMOPHILA;

EID: 0031948655     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.66.5.2245-2255.1998     Document Type: Article

References (37)

1. Berger, K. H., and R. R. Isberg. 1993. Two distinct defects in intracellular growth complemented by a single genetic locus in Legionella pneumnophila. Mol. Microbiol. 7:7-19.
2. Berger, K. H., J. J. Merriam, and R. R. Isberg. 1994. Altered intracellular targeting properties associated with mutations in the Legionella dotA gene. Mol. Microbiol. 14:809-822.
3. Borck, K., J. S. Beggs, W. J. Brammar, A. S. Hopkins, and N. E. Murry. 1976. The construction in vitro of transducing derivatives of phage λ. Mol. Gen. Genet. 146:199-207.
4. Brand, B. C., A. B. Sadosky, and H. A. Shuman. 1994. The Legionella pneumophila icm locus: a set of genes required for intracellular multiplication in human macrophages. Mol. Microbiol. 14:797-808.
5. Cianciotto, N. P., B. I. Eisenstein, C. H. Mody, and N. C. Engleberg. 1990. A mutation in the mip gene results in an attenuation of Legionella pneumophila virulence. J. Infect. Dis. 162:121-126.
6. Cianciotto, N. P., B. I. Eisenstein, C. H. Mody, G. B. Toews, and N. C. Engleberg. 1989. A Legionella pneumophila gene encoding a species-specific surface protein potentiates the initiation of intracellular infection. Infect. Immun. 57:1255-1262.
7. Clemens, D. L., and M. A. Horwitz. 1992. Membrane sorting during phagocytosis: exclusion of MHC molecules but not complement receptor CR3 during conventional and coiling phagocytosis. J. Exp. Med. 175:1317-1326.
8. Collins, S. J., F. W. Ruscetti, R. E. Gallagher, and R. C. Gallo. 1978. Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc. Natl. Acad. Sci. USA 75:2458-2462.
9. Derbyshire, K. M. 1995. An IS903-based vector for transposon mutagenesis and the isolation of gene fusions. Gene 165:143-144.
10. Feeley, J. C., R. J. Gibson, G. W. Gorman, N. C. Langford, J. K. Rasheed, D. C. Mackel, and W. B. Baine. 1979. Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila. J. Clin. Microbiol. 10:437-441.
11. Fischer, G., H. Bang, B. Ludwig, K. Mann, and J. Hacker. 1992. Mip protein of Legionella pneumophila exhibits peptidyl-prolyl-cis/trans isomerase (PPIase) activity. Mol. Microbiol. 6:1375-1383.
12. Fraser, D. W., T. R. Tsai, W. Orenstin, W. E. Parken, H. J. Beechan, R. G. Sharrar, J. Harrism, G. F. Mallison, S. M. Martin, J. E. McDade, C. C. Shepard, and P. S. Brachman. 1977. Legionnaires' disease: description of an epidemic of pneumonia. N. Engl. J. Med. 297:1189-1197.
13. Goodrich, J. A., M. L. Schwartz, and W. R. McClure. 1990. Searching for and predicting the activity of sites for DNA binding proteins: compilation and analysis of the binding sites for Escherichia coli integration host factor (IHF). Nucleic Acids Res. 18:4993-5000.
14. Horwitz, M. A. 1983. Formation of a novel phagosome by the Legionnaires' disease bacterium (Legionella pneumophila) in human monocytes. J. Exp. Med. 158:1319-1331.
15. Horwitz, M. A. 1983. The Legionnaires' disease bacterium (Legionella pneumophila) inhibits phagosome-lysosome fusion in human monocytes. J. Exp. Med. 158:2108-2126.
16. Horwitz, M. A. 1984. Phagocytosis of the Legionnaires' disease bacterium (Legionella pneumophila) occurs by a novel mechanism: engulfment within a pseudopod coil. Cell. 36:27-33.
17. Horwitz, M. A., and S. C. Silverstein. 1980. Legionnaires' disease bacterium (Legionella pneumophila) multiplies intracellularly in human monocytes. J. Clin. Invest. 60:441-150.
18. Horwitz, M. A., and S. C. Silverstein. 1983. Intracellular multiplication of Legionnaires' disease bacteria (Legionella pneumophila) in human monocytes is reversibly inhibited by erythromycin and rifampin. J. Clin. Invest. 71:15-26.
19. Imai, Y., Y. Matsushima, T. Sugimura, and M. Terada. 1991. A simple and rapid method for generating a deletion by PCR. Nucleic Acids Res. 19:2785.
20. Karlin, S., and S. F. Altschul. 1990. Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes. Proc. Natl. Acad. Sci. USA 87:2264-2268.
21. Kaufmann, A. F., J. E. McDade, C. M. Patton, J. V. Bennett, P. Skaliy, J. C. Feeley, D. C. Anderson, M. E. Potter, V. F. Newhouse, M. B. Gregg, and P. S. Brachman. 1981. Pontiac fever: isolation of the etiologic agent (Legionella pneumophila) and demonstration of its mode of transmission. Am. J. Epidemiol. 114:337-347.
22. Kyte, J., and R. F. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132.
23. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
24. Marra, A., S. J. Blander, M. A. Horwitz, and H. A. Shuman. 1992. Identification of a Legionella pneumophila locus required for intracellular multiplication in human macrophages. Proc. Natl. Acad. Sci. USA 89:9607-9611.
25. Marra, A., M. A. Horwitz, and H. A. Shuman. 1990. The HL-60 model for the interaction of human macrophages with the Legionnaires' disease bacterium. J. Immunol. 144:2738-2744.
26. Miller, J. H. 1972. Experiments in molecular biology. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
27. Morales, V. M., A. Backman, and M. Bagdasarian. 1991. A series of widehost-range low-copy-number vectors that allow direct screening for recombinants. Gene 97:39-47.
28. Nakai, K., and M. Kanehisa. 1991. Expert system for prediction of protein localization sites in gram negative bacteria. Protein Struct. Funct. Genet. 11:95-110.
29. Roy, C. R., and R. R. Isberg. 1997. Topology of Legionella pneumophila DotA: an inner membrane protein required for replication in macrophages. Infect. Immun. 65:571-578.
30. Sadosky, A. B., L. A. Wiater, and H. A. Shuman. 1993. Identification of Legionella pneumophila genes required for growth within and killing of human macrophages. Infect. Immun. 61:5361-5373.
31. Saier, M. H., Jr. Personal communication.
32. Segal, G., M. Purcell, and H. A. Shuman. 1998. Host cell killing and bacterial conjugation require overlapping sets of genes within a 22-kb region of the Legionella pneumophila genome. Proc. Natl. Acad. Sci. USA 95:1669-1674.
33. Segal, G., and H. A. Shuman. 1997. Characterization of a new region required for macrophage killing by Legionella pneumophila. Infect. Immun. 65:5057-5066.
34. Swanson, M. S., and R. R. Isberg. 1995. Association of Legionella pneumophila with the macrophage endoplasmic reticulum. Infect. Immun. 63:3609-3620.
35. Wiater, L. A., A. B. Sadosky, and H. A. Shuman. 1994. Mutagenesis of Legionella pneumophila using Tn903dII/acZ: identification of a growth-phase-regulated pigmentation gene. Mol. Microbiol. 11:641-653.
36. Wiater, L. A., K. Dunn, F. R. Maxfield, and H. A. Shuman. Unpublished data.
37. Woodcock, D. M., P. J. Crowther, J. Doherty, S. Jefferson, E. DeCruz, M. Nayer-Weidner, S. S. Smith, M. Z. Michael, and M. W. Graham. 1989. Quantitation evaluation of Escherichia coli for tolerance of cytosine methylation in plasmid and phage recombination. Nucleic Acids Res. 9:3469-3478.