Strong natural pausing by RNA polymerase II within 10 bases of transcription start may result in repeated slippage and reextension of the nascent RNA

Molecular and Cellular Biology

Volumn 22, Issue 1, 2002, Pages 30-40

  Pal, M ^a   Luse, D S ^a  

^a LERNER RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BROXURIDINE; NUCLEOSIDE TRIPHOSPHATE; RNA; RNA POLYMERASE II; URIDINE;

ARTICLE; DNA RNA HYBRIDIZATION; ENZYME LOCALIZATION; GENETIC TRANSCRIPTION; PRIORITY JOURNAL; RNA SYNTHESIS; TRANSCRIPTION INITIATION;

ADENOSINE TRIPHOSPHATE; CYTIDINE TRIPHOSPHATE; GUANOSINE TRIPHOSPHATE; HELA CELLS; HUMANS; PROMOTER REGIONS (GENETICS); RNA; RNA POLYMERASE II; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION, GENETIC; URIDINE TRIPHOSPHATE;

EID: 0036132904     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.22.1.30-40.2002     Document Type: Article

References (39)

1. Interaction of a nascent RNA structure with RNA polymerase is required for hairpin-dependent transcriptional pausing but not for transcript release
2. Two modes of transcription initiation in vitro at the rmB P1 promoter of Escherichia coli
3. Determination of sequences at the capped 5′ ends of polyoma virus early region transcripts synthesized in vivo and in vitro demonstrates an unusual microheterogeneity
4. Purified RNA polymerase II recognizes specific termination sites during transcription in vitro
5. Mechanism of transcription initiation and promoter escape by RNA polymerase II
6. Promoter escape by RNA polymerase II - A role for an ATP cofactor in suppression of arrest by polymerase at promoter-proximal sites
7. A role for TFIIH in controlling the activity of early RNA polymerase II elongation complexes
8. Structural basis of transcription: An RNA polymerase II elongation complex at 3.3 A resolution
9. Increased accommodation of nascent RNA in a product site on RNA polymerase II during arrest
10. Heterogeneous initiation due to slippage at the bacteriophage 82 late gene promoter in vitro
11. Three transitions in the RNA polymerase II transcription complex during initiation
12. RNA polymerase II ternary complexes may become arrested after transcribing to within 10 bases of the end of linear templates
13. Pseudo-templated transcription by Escherichia coli RNA polymerase at a mutant promoter
14. Slippage synthesis at the galP2 promoter of Escherichia coli and its regulation by UTP concentration and cAMP · cAMP receptor protein
15. Studies of in vitro transcription by calf thymus RNA polymerase II using a novel duplex DNA template
16. The 8-nucleotide-long RNA-DNA hybrid is a primary stability determinant of the RNA polymerase II elongation complex
17. A structural model of transcription elongation
18. A kinetic model for the early steps of RNA synthesis by human RNA polymerase II
19. RNA polymerase slides home: Pause and termination site recognition
20. Transcription-shifting RNA polymerase into overdrive
21. RNA polymerase clamps down
22. Mapping and characterization of transcriptional pause sites in the early genetic region of bacteriophage T7
23. RNA polymerase II elongation complexes paused after the synthesis of 15- or 35-base transcripts have different structures
24. Promoter-associated pausing in promoter architecture and postinitiation transcriptional regulation
25. Regulation of pyrBI operon expression in Escherichia coli by UTP-sensitive reiterative RNA synthesis during transcriptional initiation
26. Transcription elongation: Structural basis and mechanisms
27. Transcription processivity: Protein-DNA interactions holding together the elongation complex
28. The RNA-DNA hybrid maintains the register of transcription by preventing backtracking of RNA polymerase
29. Promoter clearance by RNA polymerase II is an extended, multistep process strongly affected by sequence
30. Promoter proximal sequences modulate RNA polymerase II elongation by a novel mechanism
31. Mechanism and regulation of transcriptional elongation by RNA polymerase II
32. Translocation and transcriptional arrest during transcript elongation by RNA polymerase II
33. Structural changes in the RNA polymerase II transcription complex during transition from initiation to elongation
34. Topology of the product binding site in RNA polymerase revealed by transcript slippage at the phage lambda PL promoter
35. Basic mechanisms of transcript elongation and its regulation
36. Mechanism of intrinsic transcription termination and antitermination
37. Recycling of the general transcription factors during RNA polymerase II transcription
38. Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 Å resolution