Quantitative assay for measuring the Taura syndrome virus and yellow head virus load in shrimp by real-time RT-PCR using SYBR Green chemistry

Journal of Virological Methods

Volumn 104, Issue 1, 2002, Pages 69-82

  Dhar, Arun K ^a   Roux, Michelle M ^a   Klimpel, Kurt R ^a  

^a Super Shrimp Inc   (United States)

Author keywords

Real time PCR; Shrimp; SYBR Green RT PCR; Taura syndrome virus; Yellow head virus

Indexed keywords

BETA ACTIN; DOUBLE STRANDED DNA; ELONGATION FACTOR 1ALPHA; FLUORESCENT DYE; PLASMID DNA; VIRUS DNA; VIRUS RNA;

AQUACULTURE; ARTICLE; BIOASSAY; BIOCHEMISTRY; CONCENTRATION RESPONSE; CONTROLLED STUDY; FLUORESCENCE; GENE AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA VIRUS; SAMPLING; SHRIMP; TAURA SYNDROME VIRUS; VIRUS DETECTION; VIRUS LOAD; YELLOW HEAD VIRUS;

ACTINS; ANIMALS; DECAPODA (CRUSTACEA); DNA, VIRAL; FLUORESCENT DYES; ORGANIC CHEMICALS; PEPTIDE ELONGATION FACTOR 1; PLASMIDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA VIRUS INFECTIONS; RNA VIRUSES; SENSITIVITY AND SPECIFICITY; TEMPLATES, GENETIC; TIME FACTORS; VIRAL LOAD;

DECAPODA (CRUSTACEA); PENAEIDAE; PENAEUS; PENAEUS SP.; RNA VIRUSES; TAURA SYNDROME VIRUS; TOBACCO STREAK VIRUS; YELLOW HEAD VIRUS;

EID: 0036094013     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(02)00042-3     Document Type: Article

References (28)

1. Taura syndrome of marine penaeid shrimp: Characterization of the viral agent
2. Special topic review: Taura syndrome, a disease important to shrimp farms in the Americas
3. An overview of Taura syndrome, an important disease of farmed Penaeus vannamei
4. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
5. Histology and ultrastructure reveal a new granulosis-like virus in Penaeus monodon affected by yellow-head disease
6. Detection and quantification of infectious hypodermal and hematopoietic necrosis virus and white spot virus in shrimp using real-time quantitative PCR and SYBR Green chemistry
7. Use of mRNA differential display to identify the differentially expressed genes from white spot virus infected shrimp (Penaeus stylirostris)
8. Special topic review: Major viral diseases of the black tiger prawn (Penaeus monodon) in Thailand
9. Taura syndrome in Penaeus vannamei: Demonstration of a viral etiology
10. RAPD markers as predictors of infectious hypodermal and hematopoietic necrosis virus (IHHNV) resistance in shrimp (Penaeus stylirostris)
11. Sindrome de Taura
12. Transcript quantitation in total yeast RNA using kinetic PCR
13. Quantitation of HCV RNA using real-time PCR and fluorimetry
14. Quantitative real-time PCR for the measurement of feline cytokine mRNA
15. Risk of spread of penaeid shrimp viruses in the Americas by the international movement of live and frozen shrimp
16. Shrimp diseases and current diagnostic methods
17. Measuring infectious bursal disease virus RNA in blood by multiplex real-time quantitative RT-PCR
18. Reverse transcription polymerase chain reaction (RT-PCR) used for the detection of Taura Syndrome Virus (TSV) in experimentally infected shrimp
19. Development of a novel real-time RT-PCR assay for quantitation of foot-and-mouth disease virus in diverse porcine tissues
20. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
21. Real-time RT-PCR fluorescent detection of tomato spotted wilt virus
22. Nucleotide sequence of 3′-end of the genome of Taura syndrome virus of shrimp suggests that it is related to insect picornaviruses
23. Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: Comparison of endpoint and real-time methods
24. A yellow head virus gene probe: Application to in situ hybridization and determination of its nucleotide sequence
25. Taura syndrome in Pacific white shrimp Penaeus vannamei cultured in Taiwan
26. Yellow head complex viruses: Transmission cycles and topographical distribution in the Asia-Pacific region
27. Yellow-head virus of Penaeus monodon is an RNA virus