Quantitation of HCV RNA using real-time PCR and fluorimetry

Journal of Virological Methods

Volumn 95, Issue 1-2, 2001, Pages 111-119

  Komurian Pradel, Florence ^a   Paranhos Baccalà, Gláucia ^a   Sodoyer, Mireille ^a   Chevallier, Philippe ^b   Mandrand, Bernard ^a   Lotteau, Vincent ^a   André, Patrice ^b  

^a BIOMÉRIEUX   (France)

^b Laboratoire de Virologie Est   (France)

Author keywords

HCV RNA; Quantitation; Real time PCR

Indexed keywords

DNA; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; DEVICE; DNA STRAND; FLUOROMETRY; GENE LOCATION; GENOTYPE; HEPATITIS C VIRUS; INTERMETHOD COMPARISON; MELTING POINT; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; SERUM; TEMPERATURE; VIRUS TITRATION;

FLUOROMETRY; HEPACIVIRUS; HUMANS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, VIRAL;

HEPATITIS C VIRUS;

EID: 0034997942     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(01)00300-7     Document Type: Article

References (41)

1. Evaluation of branched DNA signal amplification for the detection of hepatitis C virus RNA
2. Epidemiology of hepatitis C
3. Absolute mRNA quantification using the polymerase chain reaction (PCR): A novel approach by a PCR aided transcript titration assay (PATTY)
4. Automated quantitative determination of hepatitis C virus viremia by reverse transcription-PCR
5. The role of hepatitis C virus in hepatocellular carcinoma. Recent results
6. Interferon alfa-2b alone or in combination with ribavirin for the treatment of relapse of chronic hepatitis C.
7. Accurate quantification of hepatitis C virus (HCV) RNA from all HCV genotypes by using branched-DNA technology
8. K-tuple frequency in the human genome and polymerase chain reaction
9. Comparative study of different standardization concepts in quantitative competitive reverse transcription-PCR assays
10. Quantitation of hepatitis C virus RNA in serum of asymptomatic blood donors and patients with type C chronic liver disease
11. Comparison of plasma virus loads among individuals infected with hepatitis C virus (HCV) genotypes 1, 2, and 3 by Quantiplex HCV RNA assay versions 1 and 2, Roche Monitor assay, and an in-house limiting dilution method
12. Real time quantitative PCR
13. Hepatitis C virus quantitation: Optimization of strategies for detecting low-level viremia
14. Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase
15. Management of hepatitis C: Current and future perspectives
16. Quantitative measurement of HCV RNA in the serum: A comparison of three assays based on different principles
17. Quantitation of hepatitis C virus RNA by competitive polymerase chain reaction
18. Quantification of hepatitis C virus by TaqMan PCR: Comparison with HCV amplicor monitor assay
19. Branched DNA (bDNA) quantitation of hepatitis C viral RNA in patient sera
20. Strategy for the maximization of clinically relevant information from hepatitis C virus, RT-PCR quantification
21. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions
22. High-throughput real-time reverse transcription-PCR quantitation of hepatitis C virus
23. Interferon alfa-2b alone or in combination with ribavirin as initial treatment for chronic hepatitis C
24. Genotype dependence of hepatitis C virus load measurement in commercially available quantitative assays
25. Simultaneous screening for HBV DNA and HCV RNA genomes in blood donations using a novel TaqMan PCR assay
26. Clinical significance of hepatitis C virus genotypes
27. Rapid reverse transcription PCR detection of hepatitis C virus RNA in serum by using the Taqman fluorogenic detection system
28. Comparative study of a modified competitive RT-PCR and amplicor HCV Monitor assays for quantitation of hepatitis C virus RNA in serum
29. Comparison of two quantitative hepatitis C virus reverse transcriptase PCR assays
30. Quantification of hepatitis C virus RNA by RT-PCR in comparison to the branched DNA method
31. Establishment of the first international standard for nucleic acid amplification technology (NAT) assays for HCV RNA
32. Hepatitis C quantification and sequencing in blood products, haemophiliacs, and drug users
33. Real-time detection system for quantitation of hepatitis C virus genome
34. Branched DNA amplification multimers for the sensitive, direct detection of human hepatitis viruses
35. Viral kinetics can predict early response to alpha-interferon in chronic hepatitis
36. Global surveillance and control of hepatitis C
37. Early HCV RNA values after interferon predict response
38. Continuous fluorescence monitoring of rapid cycle DNA amplification
39. The LightCycler™: A microvolume multisample fluorimeter with rapid temperature control
40. Detection of hepatitis C virus RNA by a combined reverse transcription PCR assay: Comparison with nested amplification and antibody testing
41. Reliability of polymerase chain reaction for detection of hepatitis C virus