Chimeras of the rat and human FSH receptors (FSHRs) identify residues that permit or suppress transmembrane 6 mutation-induced constitutive activation of the FSHR via rearrangements of hydrophobic interactions between helices 6 and 7

Molecular Endocrinology

Volumn 16, Issue 8, 2002, Pages 1881-1892

  Tao, Ya Xiong ^a   Mizrachi, Dario ^a   Segaloff, Deborah L ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FOLLITROPIN RECEPTOR; G PROTEIN COUPLED RECEPTOR; LUTEINIZING HORMONE RECEPTOR; HYBRID PROTEIN;

ANIMAL CELL; ARTICLE; CHIMERA; COMPUTER MODEL; GENE MUTATION; HORMONE RECEPTOR INTERACTION; HUMAN; HUMAN CELL; HYDROPHOBICITY; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; RAT; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL; CELL LINE; CHEMICAL STRUCTURE; CHEMISTRY; COMPARATIVE STUDY; COMPUTER SIMULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE; SPECIES DIFFERENCE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL; CELL LINE; CHIMERIC PROTEINS; COMPARATIVE STUDY; COMPUTER SIMULATION; HUMAN; HYDROPHOBICITY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; RATS; RECEPTORS, FSH; SPECIES SPECIFICITY; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; ANIMALS; HUMANS; RECOMBINANT FUSION PROTEINS;

ANIMALIA;

EID: 0036020508     PISSN: 08888809     EISSN: None     Source Type: Journal    
DOI: 10.1210/me.2001-0199     Document Type: Article

References (32)

1. Identification of the sites of N-linked glycosylation on the follicle-stimulating hormone (FSH) receptor and assessment of their role in FSH receptor function
2. Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a G protein-coupled, seven transmembrane region
3. The three subfamilies of leucine-rich repeat-containing G protein-coupled receptors (LGR): Identification of LGR6 and LGR7 and the signaling mechanism for LGR7
4. Truncation of the C-terminal tail of the follitropin receptor does not impair the agonist- or phorbol ester-induced receptor phosphorylation and uncoupling
5. Uncovering molecular mechanisms involved in activation of G protein-coupled receptors
6. 2-adrenergic receptor: Extending the ternary complex model
7. Constitutive activation of a single effector pathway: Evidence for multiple activation states of a G protein-coupled receptor
8. 1-adrenergic receptor activation
9. Constitutive activity of glucagon receptor mutants
10. Agonist regulation of D2 dopamine receptor/G protein interaction, evidence for agonist selection of G protein subtype
11. An activating mutation of the follicle-stimulating hormone receptor autonomously sustains spermatogenesis in a hyophysectomized man
12. Naturally occurring mutations of the luteinizing-hormone receptor: Lessons learned about reproductive physiology and G protein-coupled receptors
13. The human thyrotropin receptor is highly mutable: A review of gain-of-function mutations
14. No evidence of a role for mutations or polymorphisms of the follicle-stimulating hormone receptor in ovarian granulosa cell tumors
15. Analysis of mutations in genes of the follicle-stimulating hormone receptor signaling pathway in ovarian granulosa cell tumors
16. Transmembrane regions V and VI of the human luteinizing hormone receptor are required for constitutive activation by a mutation in the third intracellular loop
17. A constitutively activating mutation of the luteinizing hormone receptor in familial male precocious puberty
18. Constitutive activation of the human vasoactive intestinal peptide 1 receptor, a member of the new class II family of G protein-coupled receptors
19. Crystal structure of rhodopsin: A G protein-coupled receptor
20. Mutations of gonadotropins and gonadotropin receptors: Elucidating the physiology and pathophysiology of pituitary-gonadal function
21. Somatic and germline mutations of the TSH receptor and thyroid diseases
22. Genetic heterogeneity of constitutively activating mutations of the human luteinizing hormone receptor in familial male-limited precocious puberty
23. An α-carbon template for the transmembrane helices in the rhodopsin family of G-protein-coupled receptors
24. Structural mimicry in G protein-coupled receptors: Implications of the high-resolution structure of rhodopsin for structure-function analysis of rhodopsin-like receptors
25. Seven-transmembrane receptors: Crystals clarify
26. 2 adrenergic receptor mapped by site-selective fluorescent labeling
27. Hinges, swivels and switches: The role of prolines in signalling via transmembrane α-helices
28. Geometrical and sequence characteristics of α-helices in globular proteins
29. Gonadotropin binding and stimulation of steroidogenesis in Leydig tumor cells
30. Site-directed mutagenesis by overlap extension using the polymerase chain reaction
31. Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension
32. High-efficiency transformation of mammalian cells by plasmid DNA