Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation

Journal of Molecular Biology

Volumn 311, Issue 4, 2001, Pages 777-787

  Agrawal, Rajendra K ^a,b   Linde, Jamie ^a   Sengupta, Jayati ^a   Nierhaus, Knud H ^d   Frank, Joachim ^a,b,c  

^a WADSWORTH CENTER   (United States)

^b STATE UNIVERSITY OF NEW YORK   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^d MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

Author keywords

Elongation factor release; Thiostrepton; Translation

Indexed keywords

BACTERIAL PROTEIN; ELONGATION FACTOR; PROTEIN L 11; THIOSTREPTON; UNCLASSIFIED DRUG;

ARTICLE; ESCHERICHIA COLI; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEIN SECRETION; RIBOSOME; X RAY CRYSTALLOGRAPHY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0035943352     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4907     Document Type: Article

References (54)

1. The NH2-terminal domain of Escherichia coli ribosomal protein L11. Its three-dimensional location and its role in the binding of release factors 1 and 2
2. Ribosomes in thiostrepton-resistant mutants of Bacillus megaterium lacking a single 50 S subunit protein
3. Mutants of Escherichia coli lacking ribosomal protein L11
4. Binding of thiostrepton to a complex of 23-S rRNA with ribosomal protein L11
5. Recognition of the highly conserved GTPase center of 23 S ribosomal RNA by ribosomal protein L11 and the antibiotic thiostreptan
6. Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23 S RNA
7. Visualization of tRNA movements on the Escherichia coli 70 S ribosome during the elongation cycle
8. Involvement of specific proteins of ribosomal RNA in defined ribosomal functions: A study utilizing antibiotics
9. Mapping the position of translational elongation factor EF-G in the ribosome by directed hydroxyl radical probing
10. Visualization of elongation factor G on the Escherichia coli 70 S ribosome: The mechanism of translocation
11. EF-G-dependent GTP hydrolysis induces translocation accompanied by large conformational changes in the 70 S ribosome
12. Studies of elongation factor G-dependent tRNA translocation by three-dimensional cryo-electron microscopy
13. Large-scale movement of elongation factor G and extensive conformational change of the ribosome during translocation
14. Visualization of elongation factor Tu on the Escherichia coli ribosome
15. Phe, EF-TU, and a GTP analog
16. Structure of the ternary complex of EF-Tu: Macromolecular mimicry in translation
17. A complex profile of protein elongation: Translating chemical energy into molecular movement
18. Three-dimensional structure of the ribosomal translocase: Elongation factor G from Thermus thermophilus
19. The crystal structure of elongation factor G complexed with GDP, at 2.7 Å resolution
20. The structure of elongation factor G in complex with GDP: Conformational flexibility and nucleotide exchange
21. Crystal structure of a conserved ribosomal protein-RNA complex
22. A detailed view of a ribosomal active site: The structure of the L11-RNA complex
23. Cooperative interactions of RNA and thiostrepton antibiotic with two domains of ribosomal protein L11
24. The antibiotic thiostrepton inhibits a functional transition within protein L11 at the ribosomal GTPase center
25. The antibiotic micrococcin acts on protein L11 at the ribosomal GTPase center
26. Placement of protein and RNA structures into a 5 Å-resolution map of the 50 S ribosomal subunit
27. Domain motions of EF-G bound to the 70 S ribosome: Insights from a hand-shaking between multi-resolution structures
28. Solution structure of the E. coli 70 S ribosome at 11.5 Å resolution
29. The Escherichia coli large ribosomal subunit at 7.5 Å resolution
30. The 3D arrangement of the 23 S and 5 S rRNA in the E. coli ribosomal subunit based on a cryo-electron microscopic reconstruction at 7.5 Å resolution
31. The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution
32. Immumological studies of Escherichia coli mutants lacking one or two ribosomal proteins
33. Met and fitting of L1 protein
34. Structural mechanisms for domain movements in proteins
35. A database of macromolecular motions
36. A ratchet-like inter-subunit reorganization of the ribosome during translocation
37. Three-dimensional cryo-electron microscopy localization of EF2 in the Saccharomyces cerevisiae 80 S ribosome at 17.5 Å resolution
38. Mapping of ribosomal RNA neighborhood of protein L11 by directed hydroxyl radical probing
39. The RNA binding domain of ribosomal protein L11: Three-dimensional structure of the RNA-bound form of the protein and its interaction with 23 S rRNA
40. Location and domain structure of Escherichia coli ribosomal protein L7/L12: Site-specific cysteine crosslinking and attachment of fluorescent probes
41. Conformationally restricted elongation factor-G retains GTPase activity but is inactive in translocation
42. Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome
43. Structure of functionally activated small ribosomal subunit at 3.3 Å resolution
44. Structure of the 30 S ribosomal subunit
45. Movement of the decoding region of the 16 S ribosomal RNA accompanies tRNA translocation
46. Role of domains 4 and 5 in elongation factor G functions on the ribosome
47. Electron microscopy and computer image averaging of ice-embedded large ribosomal subunits from Escherichia coli
48. Automatic particle picking from electron micrographs
49. Three-dimensional reconstruction with contrast transfer function correction from energy-filtered cryoelectron micrographs: Procedure and application to the 70 S Escherichia coli ribosome
50. The ribosome at improved resolution: New techniques for merging and orientation refinement in 3D cryo-electron microscopy of biological particles
51. Structure of keyhole limpet hemocyanin type 1 (KLH1) at 15 Å resolution by electron cryomicroscopy and angular reconstitution
52. Improved methods for building protein models in electron density maps and the location of errors in these models
53. Fitting atomic models into electron-microscopy maps
54. Ribbons