Ribosomal peptidyl transferase can withstand mutations at the putative catalytic nucleotide

Nature

Volumn 411, Issue 6836, 2001, Pages 498-501

  Polacek, N ^a   Gaynor, M ^a   Yassin, A ^a   Mankin, A S ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; BIOSYNTHESIS; CRYSTAL STRUCTURE; GENES; POLYPEPTIDES; RNA;

MUTATIONS;

ENZYMES;

NUCLEOTIDE; PEPTIDYLTRANSFERASE; RIBOSOME RNA;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RIBOSOME SUBUNIT; RNA STRUCTURE; THERMUS AQUATICUS;

ADENINE; BASE SEQUENCE; CATALYSIS; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEOTIDES; PEPTIDYL TRANSFERASES; RIBOSOMES; RNA, CATALYTIC; RNA, RIBOSOMAL, 23S; THERMUS;

ESCHERICHIA COLI; NEGIBACTERIA; THERMUS AQUATICUS;

EID: 0035942753     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/35078113     Document Type: Article

References (30)

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4. Interaction of tRNA with 23S rRNA in the ribosomal A, P, and E sites
5. 23S rRNA positions essential for tRNA binding in ribosomal functional sites
6. Photo-affinity labelling at the peptidyl transferase centre reveals two different positions for the A- and P-sites in domain V of 23S rRNA
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12. Oxazolidinone resistance mutations in 23S rRNA of Escherichia coli reveal the central region of domain V as the primary site of drug action
13. Reconstitution of functional 50S ribosomes from in vitro transcripts of Bacillus stearothermophilus 23S rRNA
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15. Mapping important nucleotides in the peptidyl transferase centre of 23 S rRNA using a random mutagenesis approach
16. Mutations at U2555, a tRNA-protected base in 23S rRNA, affect translational fidelity
17. General acid-base catalysis in the mechanism of a hepatitis delta virus ribozyme
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19. The peptidyltransferase center of ribosomes
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22. Peptidyl transferase activity catalyzed by protein-free 23S ribosomal RNA remains elusive
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