Indirect and direct evidence for DNA double-strand breaks in hypermutating immunoglobulin genes

Philosophical Transactions of the Royal Society B: Biological Sciences

Volumn 355, Issue 1405, 2001, Pages 119-125

  Jacobs, H ^a   Rajewsky, K ^a   Fukita, Y ^a   Bross, L ^a  

^a BASEL INSTITUTE FOR IMMUNOLOGY   (Switzerland)

Author keywords

Deletion ends; Germinal centre; Knock in mice; Ligation mediated polymerase chain reaction; Non homologous DNA double strand break repair; RGYW motif

Indexed keywords

DNA STRAND BREAKAGE; DOUBLE STRANDED DNA; GENE DELETION; GENE INSERTION; GENETIC STRAIN; GENETIC TRANSCRIPTION; IMMUNE SYSTEM; IMMUNOGLOBULIN GENE; LYMPHOCYTE ANTIGEN RECEPTOR; MOUSE; POINT MUTATION; PROMOTER REGION; SOMATIC MUTATION;

DNA; IMMUNOGLOBULIN;

ANIMAL; ARTICLE; B LYMPHOCYTE; C57BL MOUSE; CHROMOSOME INVERSION; CHROMOSOME MAP; DNA DAMAGE; GENE DELETION; GENETICS; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR GENETICS; MOUSE; MUTATION; NUCLEOTIDE SEQUENCE; PROMOTER REGION; TRANSGENIC MOUSE;

ANIMALS; B-LYMPHOCYTES; BASE SEQUENCE; CHROMOSOME MAPPING; DNA; DNA DAMAGE; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOGLOBULINS; INVERSION, CHROMOSOME; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; MOLECULAR SEQUENCE DATA; MUTATION; PROMOTER REGIONS (GENETICS); SEQUENCE DELETION;

EID: 0035966778     PISSN: 09628436     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (27)

1. Timing, genetic requirements and functional consequences of somatic hypermutation during B-cell development
2. Maturation of the immune response in germinal centers
3. Passenger transgenes reveal intrinsic specificity of the antibody hypermutation mechanism: Clustering, polarity, and specific hot spots
4. Elements regulating somatic hypermutation of an immunoglobulin kappa gene: Critical role for the intron enhancer/matrix attachment region
5. Hot spots of somatic hypermutation are preferred targets of DNA double-strand breaks
6. Splinkerettes-improved vectorettes for greater efficiency in PCR walking
7. Somatic hypermutation of human immunoglobulin heavy chain genes: Targeting of RGYW motifs on both DNA strands
8. Somatic hypermutation in the heavy chain locus correlates with transcription
9. Frequent occurrence of deletions and duplications during somatic hypermutation: Implications for oncogene translocations and heavy chain disease
10. Intraclonal generation of antibody mutants in germinal centres
11. Tuning somatic hypermutation by transcription
12. H and VL genes, minigenes, and complementarity-determining regions to binding of antibody-combining sites
13. A foreign beta-globin gene in transgenic mice: Integration at abnormal chromosomal positions and expression in inappropriate tissues
14. In vivo ablation of surface immunoglobulin on mature B cells by inducible gene targeting results in rapid cell death
15. Boundaries of somatic mutation in rearranged immunoglobulin genes: 5′ Boundary is near the promoter, and 3′ boundary is approximately 1 kb from V(D)J gene
16. Generation of antibody diversity in the immune response of BALB/c mice to influenza virus hemagglutinin
17. Germinal centers
18. Both DNA strands of antibody genes are hypermutation targets
19. Somatic hypermutation of immunoglobulin genes is linked to transcription initiation
20. Somatic hypermutagenesis in immunoglobulin genes. II. Influence of neighbouring base sequences on mutagenesis
21. Somatic hypermutation in 5′ flanking regions of heavy chain antibody variable regions
22. TdT-accessible breaks are scattered over the immunoglobulin V domain in a constitutively hypermutating B cell line
23. Natural occurrence and origin of somatically mutated memory B cells in mice
24. The transcriptional promoter regulates hypermutation of the antibody heavy chain locus
25. Position of the rearranged V kappa and its 5′ flanking sequences determines the location of somatic mutations in the J kappa locus
26. The repertoire of somatic antibody mutants accumulating in the memory compartment after primary immunization is restricted through affinity maturation and mirrors that expressed in the secondary response
27. Targeting of non-Ig sequences in place of the V segment by somatic hypermutation