Nucleolar assembly of the rRNA processing machinery in living cells

Journal of Cell Biology

Volumn 153, Issue 5, 2001, Pages 1097-1110

  Savino, Tulia Maria ^a   Gébrane Younès, Jeannine ^a,b   De Mey, Jan ^c   Sibarita, Jean Baptiste ^c   Hernandez Verdun, Danièle ^a  

^a INSTITUT JACQUES MONOD   (France)

^b INSTITUT CURIE   (France)

^c INSTITUT CURIE   (France)

Author keywords

4 D imaging; Electron microscopy; Nuclear dynamics; Prenucleolar body; Time lapse microscopy

Indexed keywords

CELL PROTEIN; PROTEIN NOP52; RIBOSOME RNA; UNCLASSIFIED DRUG;

ARTICLE; CELL MIGRATION; CHROMOSOME; CONTROLLED STUDY; DYNAMICS; ELECTRON MICROSCOPY; FLUORESCENCE; HUMAN; HUMAN CELL; METAPHASE; MICROSCOPY; MITOSIS; NUCLEOLUS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN TRANSPORT; RNA PROCESSING;

BLOTTING, WESTERN; CELL NUCLEOLUS; CHROMOSOMAL PROTEINS, NON-HISTONE; FLUORESCENT ANTIBODY TECHNIQUE; G1 PHASE; GENES, RRNA; HELA CELLS; HUMANS; KINETICS; MICROSCOPY, ELECTRON; MITOSIS; NUCLEAR PROTEINS; NUCLEOLUS ORGANIZER REGION; PHOSPHOPROTEINS; PROTEIN TRANSPORT; PROTOZOAN PROTEINS; RECOMBINANT FUSION PROTEINS; RNA POLYMERASE I; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, RIBOSOMAL; TELOPHASE; TRANSFECTION;

EID: 0035947758     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.153.5.1097     Document Type: Article

References (53)

1. Fluorescence microscopy in three dimensions
2. cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera
3. Postmitotic nuclear reorganization events analyzed in living cells
4. Inhibition of nucleolar reformation after microinjection of antibodies to RNA polymerase I into mitotic cells
5. Temporal and spatial control of cyclin B1 destruction in metaphase
6. Initiation of nucleolar assembly is independent of RNA polymerase I transcription
7. Partially processed pre-rRNA is preserved in association with processing components in nucleolus derived foci during mitosis
8. A class of nonribosomal nucleolar components is located in chromosome periphery and in nucleolus-derived foci during anaphase and telophase
9. The dynamics of postmitotic reassembly of the nucleolus
10. Quantitative imaging of pre-mRNA splicing factors in living cells
11. Effects of anti-PM-Sc1 100 (Rr6p exonuclease) antibodies on prenucleolar body dynamics at the end of mitosis
12. Effects of anti-fibrillarin antibodies on building of functional nucleoli at the end of mitosis
13. When rDNA transcription is arrested during mitosis, UBF is still associated with non-condensed rDNA
14. The nucleus: A highly organized but dynamic structure
15. Segregation of the nucleolus during mitosis in budding and fission yeast
16. Link between aging and the nucleolus
17. Nopp140 functions as a molecular link between the nucleolus and the coiled bodies
18. Nucleologenesis U3 snRNA-containing prenucleolar bodies move to sites of active pre-rRNA transcription after mitosis
19. In vivo evidence that TATA-binding protein SL1 colocalizes with UBF and RNA polymerase i when rRNA synthesis is either active or inactive
20. Structure and function in the nucleus
21. Like attracts like: Getting RNA processing together in the nucleus
22. Nuclear bodies: Multifaceted subdomains of the interchromatin space
23. Origins of linear an non-linear recursive restoration algorithms
24. Cell biology of transcription and pre-mRNA splicing: Nuclear architecture meets nuclear function
25. Localization of DNA-dependent RNA polymerase activities in fixed human fibroblasts by autoradiography
26. Double immunolocalization of major nucleolar proteins, fibrillarin and B23, in dividing mammalian cultured cells
27. Nucleologenesis: Composition and fate of prenucleolar bodies
28. The nucleolus: An old factory with unexpected capabilities
29. The plurifunctional nucleolus
30. High mobility of proteins in the mammalian cell nucleus
31. Association of nonribosomal nucleolar proteins in ribonucleoprotein complexes during interphase and mitosis
32. In vivo analysis of Cajal body movement, separation, and joining in live human cells
33. Signal recognition particle components in the nucleolus
34. Monoclonal antibody from a (New Zealand black X New Zeeland White)F1 mouse and some human scleroderma sera target an Mr 34,000 nucleolar protein of the U3 RNP particle
35. Bayesian-based iterative method of image restoration
36. The rDNA transcription machinery is assembled during mitosis in active NORs and absent in inactive NORs
37. Localization of the RNA polymerase I transcription factor hUBF during the cell cycle
38. The nucleolar antigen Nop 52, the human homologue of the yeast ribosomal RNA processing RRP1, is recruited at late stages of nucleologenesis
39. Structure and function of the nucleolus
40. Structure, function and assembly of the nucleolus
41. The nucleolus
42. The mitotically phosphorylated form of the transcription termination factor TTF-1 is associated with the repressed rDNA transcription machinery
43. In vivo release of mitotic silencing of rDNA transcription does not give rise to pre-rRNA processing
44. Newly assembled snRNPs associated with coiled bodies before speckles, suggesting a nuclear snRNP maturation pathway
45. The fine structure of the nucleolus during mitosis in the grasshopper neuroblast cell
46. Net1, a Sir2-associated nucleolar protein required for rDNA silencing and nucleolar integrity
47. Presence of pre-rRNAs before activation of polymerase I transcription in the building process of nucleoli during early development of Xenopus laevis
48. The ribosomal RNA processing machinery is recruited to the nucleolar domain before RNA polymerase I during Xenopus laevis development
49. The nucleolus: The magician's hat for cell cycle tricks
50. Experimental induction of prenucleolar bodies (PNBs) in interphase cells: Interphase PNBs show similar characteristics as those typically observed at telophase of mitosis in untreated cells
51. Nucleoporins Nup98 and Nup214 participate in nuclear export of human immunodeficiency virus type 1 Rev