Unusual properties of the fungal conventional kinesin neck domain from Neurospora crassa

EMBO Journal

Volumn 20, Issue 22, 2001, Pages 6226-6235

  Kallipolitou, Athina ^a   Deluca, Dominga ^b   Majdic, Ulrike ^a   Lakämper, Stefan ^c   Cross, Robert ^d   Meyhöfer, Edgar ^c   Moroder, Luis ^b   Schliwa, Manfred ^a   Woehlke, Günther ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^c HANNOVER MEDICAL SCHOOL   (Germany)

^d MARIE CURIE RESEARCH INSTITUTE   (United Kingdom)

Author keywords

ATPase kinetics; Domain analysis; Fungal kinesin; Processivity; Synthetic peptide

Indexed keywords

ADENOSINE TRIPHOSPHATASE; KINESIN;

ARTICLE; BEHAVIOR; CATALYSIS; DELETION MUTANT; DROSOPHILA; ENZYME ACTIVITY; FUNGUS; MICROTUBULE; NEUROSPORA CRASSA; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN STRUCTURE; THERMOSTABILITY;

ANIMALIA; FUNGI; NEUROSPORA CRASSA;

EID: 0035890334     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.22.6226     Document Type: Article

References (50)

1. Enzymatic hydrolysis with carboxypeptidases
2. Estimation of molecular size and molecular weights of biological components by gel filtration
3. Hydrophobic solvation in aqueous trifluoroethanol solution
4. The coiled-coil trigger site of the rod domain of cortexillin I unveils a distinct network of interhelical and intrahelical salt bridges
5. Coupled chemical and mechanical reaction steps in a processive Neurospora kinesin
6. Imaging and nano-manipulation of single biomolecules
7. Pathway of processive ATP hydrolysis by kinesin
8. Cloning and functional expression of a 'fast' fungal kinesin
9. Importance of a flexible hinge near the motor domain in kinesin-driven motility
10. Highly processive microtubule-stimulated ATP hydrolysis by dimeric kinesin head domains
11. Processivity of the motor protein kinesin requires two heads
12. Reversal in the direction of movement of a molecular motor
13. Drosophila kinesin motor domain extending to amino acid position 392 is dimeric when expressed in Escherichia coli
14. Drosophila kinesin minimal motor domain expressed in Escherichia coli. Purification and kinetic characterization
15. Movements of truncated kinesin fragments with a short or an artificial flexible neck
16. Monomeric kinesin head domains hydrolyze multiple ATP molecules before release from a microtubule
17. Influence of the kinesin neck domain on dimerization and ATPase kinetics
18. Universal and unique features of kinesin motors: Insights from a comparison of fungal and animal conventional kinesins
19. Orientation, positional, additivity and oligomerization-state effects of interhelical ion pairs in α-helical coiled-coils
20. The crystal structure of dimeric kinesin and implications for microtubule-dependent motility
21. Identification of a motor protein required for filamentous growth in Ustilago maydis
22. Mass spectrometric studies on noncovalent dimers of leucine zipper peptides
23. Prediction and analysis of coiled-coil structures
24. Mechanism of microtubule kinesin ATPase
25. Interacting head mechanism of microtubule-kinesin ATPase
26. The structural and mechanochemical cycle of kinesin
27. Identification of kinesin neck region as a stable α-helical coiled coil and its thermodynamic characterization
28. Pathway of ATP hydrolysis by monomeric and dimeric kinesin
29. Elektrospray-massenspektrometrie von biomakromolekülkomplexen mit nichtkovalenten wechselwirkungen - Neue analytische perspektiven fuer supramolekulare chemie und molekulare erkennungsprozesse
30. Genetic evidence for a microtubule-destabilizing effect of conventional kinesin and analysis of its consequences for the control of nuclear distribution in Aspergillus nidulans
31. A structural change in the kinesin motor protein that drives motility
32. Role of the kinesin neck region in processive microtubule-based motility
33. Equilibrium studies of kinesin-nucleotide intermediates
34. Conformational preferences of a synthetic 30mer peptide from the interface between the neck and stalk regions of kinesin
35. Cargo binding and regulatory sites in the tail of fungal conventional kinesin
36. ATPase kinetic characterization and single molecule behavior of mutant human kinesin motors defective in microtubule-based motility
37. Effect of trifluoroethanol on protein secondary structure: An NMR and CD study using a synthetic actin peptide
38. The cellular roles of molecular motors in fungi
39. The Neurospora organelle motor: A distant relative of conventional kinesin with unconventional properties
40. Engineering the processive run length of the kinesin motor
41. Controlling kinesin by reversible disulfide cross-linking. Identifying the motility-producing conformational change
42. Demonstration of coiled-coil interactions within the kinesin neck region using synthetic peptides. Implications for motor activity
43. The design plan of kinesin motors
44. Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
45. The C-terminus of tubulin increases cytoplasmic dynein and kinesin processivity
46. Walking on two heads: The many talents of kinesin
47. A fungal kinesin required for organelle motility, hyphal growth and morphogenesis
48. One-headed kinesin derivatives move by a nonprocessive, low-duty ratio mechanism unlike that of two-headed kinesin