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Recent experiments in which buffer is flowed between the two coverslips suggest that obstacles are not defects in the bilayer. Subject to hydrodynamic drag, the DNA molecules are again observed to adopt hooked conformations, but the hooking point (obstacle) is now seen to translate in the direction of hydrodynamic flow. In contrast, during electrophoresis experiments, the hooking points remain stationary. It would be expected that bilayer defects would be stationary in hydro-dynamic flow and during electrophoresis. If the defects were unfused vesicles, it is possible that they could be translated in hydrodynamic flow. The obstacles could also consist of microdomains of cationic lipids. Electrophoretic forces would act in the opposite direction on cationic domains as they would on DNA chains; however, in hydrodynamic flow, there would be no counterforce acting on the domains. The lack of a counterforce could explain the mobility of cationic domains in hydro-dynamic flow as opposed to their immobility during electrophoresis.
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