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Volumn 17, Issue 23, 2001, Pages 7396-7401

Electrophoresis of DNA adsorbed to a cationic supported bilayer

Author keywords

[No Author keywords available]

Indexed keywords

CATIONIC SUPPORTED BILAYERS;

EID: 0035856668     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la010475j     Document Type: Article
Times cited : (38)

References (48)
  • 27
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    • 0011322381 scopus 로고    scopus 로고
    • note
    • Recent experiments in which buffer is flowed between the two coverslips suggest that obstacles are not defects in the bilayer. Subject to hydrodynamic drag, the DNA molecules are again observed to adopt hooked conformations, but the hooking point (obstacle) is now seen to translate in the direction of hydrodynamic flow. In contrast, during electrophoresis experiments, the hooking points remain stationary. It would be expected that bilayer defects would be stationary in hydro-dynamic flow and during electrophoresis. If the defects were unfused vesicles, it is possible that they could be translated in hydrodynamic flow. The obstacles could also consist of microdomains of cationic lipids. Electrophoretic forces would act in the opposite direction on cationic domains as they would on DNA chains; however, in hydrodynamic flow, there would be no counterforce acting on the domains. The lack of a counterforce could explain the mobility of cationic domains in hydro-dynamic flow as opposed to their immobility during electrophoresis.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.