A novel feeding strategy for enhanced plasmid stability and protein production in recombinant yeast fedbatch fermentation

Biotechnology and Bioengineering

Volumn 56, Issue 1, 1997, Pages 23-31

  Cheng, Chinyuan ^a   Huang, Yu Liang ^a   Yang, Shang Tian ^a  

^a Ohio State University   (United States)

Author keywords

Fedbatch fermentation; Plasmid stability; Protein production; Recombinant protein production

Indexed keywords

CELL CULTURE; CHEMICAL VARIABLES CONTROL; COMPUTER CONTROL; COMPUTER SIMULATION; ENZYMES; GLUCOSE; GROWTH KINETICS; SUBSTRATES; YEAST;

FEEDING STRATEGY; PLASMID STABILITY; RECOMBINANT PROTEIN PRODUCTION; SACCHAROMYCES CEREVISIAE;

FERMENTATION;

BETA GALACTOSIDASE;

ARTICLE; BINDING KINETICS; CELL DENSITY; CULTURE MEDIUM; FERMENTATION; GENETIC ENGINEERING; GLUCOSE METABOLISM; NONHUMAN; OSCILLATION; PROTEIN STABILITY; SACCHAROMYCES CEREVISIAE;

EID: 0343487769     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19971005)56:1<23::AID-BIT3>3.0.CO;2-X     Document Type: Article

References (39)

1. Bradley, J., Schmid, R. D. 1991. Optimization of biosensor for in situ fermentation monitoring of glucose concentration. Biosensors Bioelectron. 6: 669-674.
2. Caunt, P., A. Impoolsup, Greenfield, P. F. 1988. Stablity of recombinant plasmid in yeast. J. Biotechnol. 8: 173-178.
3. Caunt, P., A. Impoolsup, Greenfield, P. F. 1990. A method for the stabilization of recombinant plasmids in yeast. J. Biotechnol. 14: 311-320.
4. Cheng, C. 1992. Engineering strategies to optimize plasmid stability and protein production in recombinant Saccharomyces cerevisiae fermentation, pp. 116-121. Ph.D. dissertation, The Ohio State University, Columbus, Ohio.
5. Cheng, C., Yang, S. T. 1996. Dynamics and modeling of temperature-regulated gene product expression in a recombinant yeast fermentation. Biotechnol. Bioeng. 50: 663-674.
6. Cho, Y. N., Cheng, Y. K. 1995. On-line measurement and control of cell concentration of Saccharomyces cerevisiae using a laser turbidimeter. Biotechnol. Techniques 9: 557-562.
7. De Taxis du Poet, P., Arcand, Y., Bernier Jr., R., Barbotin, J.-N., Thomas, D. 1987. Plasmid stability in immobilized and free recombinant Escherichia coli JM105(pKK223-200): Importance of oxygen diffusion, growth rate, and plasmid copy number. Appl. Environ. Microbiol. 53: 1548-1555.
8. Dinwoodie, R. C., Mehnert, D. W. 1985. A continuous method for monitoring and controlling fermentations: Using an automated HPLC system. Biotechnol. Bioeng. 27: 1060.
9. Ejiofor, A. O., Chisti, Y., Moo Young, M. 1996. Fed-batch production of baker's yeast using millet (Pennisetum typhoides) flour hydrolysate as the carbon source. J. Industrial Microbiol. 16: 102-109.
10. Gregory, M. E., Turner, C. 1993. Open-loop control of specific growth rate in fed-batch cultures of recombinant E. coli. Biotechnol. Techniques 7: 889-894.
11. Henry, K. L., Davis, R. H., Taylor, A. L. 1990. Continuous recombinant bacterial fermentations utilizing selective flocculation and recycle. Biotechnol. Prog. 6: 7-12.
12. Hortacsu, A., Ryu, D. D. Y. 1991. Optimization of a two-stage recombinant fermentation processes: The dilution rate effect. Biotechnol. Bioeng. 38: 831-837.
13. Huang, Y. L., Li, S. Y., Dremel, B. A. A., Bilitewski, U., Schmid, R. D. 1991. On-line determination of glucose concentration throughout animal cell cultures based on chemiluminescent detection of hydrogen peroxide coupled with flow injection analysis. J. Biotechnol. 18: 161-172.
14. Huang, Y. L., Foellmer, T. J., Ang, K. C., Khoo, S. B., Yap, M. G. S. 1995. Characterization and application of an on-line injection analysis/wall jet electrode system for glucose monitoring during fermentation. Anal. Chim. Acta 317: 223-232.
15. Huang, Y. L., Shu, C. H., Yang, S. T. 1996. Kinetics and modeling of GM-CSF production by immobilized recombinant yeast in a three-phase fluidized bed bioreactor. Biotechnol. Bioeng. 53: 470-477.
16. Humphrey, A. E. 1988. Algorithmic monitors for computer control of fermentations, p. 121. In: S. Aiba (ed.), Horizons of biochemical engineering. Oxford University Press, Oxford.
17. Iminaka, T., Aiba, A. 1981. A perspective on the application of genetic engineering: Stability of recombinant plasmid. Ann. NY Acad. Sci. 369: 1-10.
18. Impoolsup, A., Caunt, P., Greenfield, P. F. 1989. Stabilization of a recombinant yeast plasmid in nonselective medium by cyclic growth rate changes. Biotechnol. Lett. 9: 605-608.
19. Kleman, G. L., Chalmers, J. J., Lulli, G. W., Strahl, W. R. 1991. Glucosestat, a glucose-controlled continuous culture. Appl. Environ. Microb. 57: 918-923.
20. Kumar, P. K. R., Schugerl, K. 1990. Immobilization of genetically engineered cells: A new strategy for higher stability. J. Biotechnol. 14: 255-272.
21. Lee, F. J. S., Hassan, H. M. 1987. Effect of oxygen tension on stability and expression of a killer toxin chimeric plasmid in a chemostat culture of Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 27: 72-74.
22. Lee, S. B., Ryu, D. D. Y., Siegel, R., Park, S. H. 1988. Performance of recombinant fermentation and evaluation of gene expression efficiency for gene product in two-stage continuous culture system. Biotechnol. Bioeng. 33: 805-820.
23. Mak, K. W. Y., Yap, M. G. S., Huang, Y. L., Teo, W. K. 1992. Growth and induction effects on the expression of rhTNF-b, p. 189. In: S. Furusaki, I. Endo and R. Matsuno (Eds.) Biochemical engineering for 2001. Springer-Verlag, Tokyo.
24. Miller, J. 1972. Experiments in molecular genetics, pp. 352. Cold Spring Harbor Laboratory, NY.
25. Montesinos, J. L., Lafuente, J., Gordillo, M. A., Valero, F., Sola, C., Charbonnier, S., Cheruy, A. 1995. Structured modelling and state estimation in a fermentation process: Lipase production by Candida rugosa. Biotechnol. Bioeng. 48: 573-584.
26. Ollis, D. F. 1982. Industrial fermentation with (unstable) recombinant cultures. Phil. Trans. R. Soc. London, Ser. B. 297: 617-629.
27. Peretti, S. W., Bailey, J. E. 1988. Transient response simulations of recombinant microbial population. Biotechnol. Bioeng. 32: 418-429.
28. Saint, A. S., Perlot, P., Goma, G., Soucaille, P. 1994. High production of 1,3-propanediol from glycerol by Glostridium butyricum VPI 3266 in a simply controlled fedbatch system. Biotechnol. Lett. 16: 831-836.
29. Seo, J. H., Bailey, J. E. 1985. Effects of recombinant plasmid content on growth properties and cloned gene product formation in E. coli. Biotechnol. Bioeng. 27: 1668-1675.
30. Shu, C. H., Yang, S. T. 1996a. Production of recombinant GM-CSF by Saccharomyces cerevisiae in a three-phase fluidized bed bioreactor. Biotechnol. Bioeng. 51: 229-236.
31. Shu, C. H., Yang, S. T. 1996b. Kinetics of Continuous GM-CSF Production by Recombinant Saccharomyces cerevisiae in an Air-Lift Bioreactor, J. Biotechnol., 48: 107-116.
32. Sledziewski, A. Z., A. Bel, K. Kelsay, Mackay, V. L. 1988. Construction of temperature-regulated yeast promoters using the MATα2 repression system. Bio/technology 6: 411-416.
33. Stephens, M. L., Lyberatos, G. 1988. Effect of cycling on the stability of plasmid-bearing microorganisms in continuous culture. Biotechnol. Bioeng. 31: 464-469.
34. Stephens, M. L., C. Christensen, Lyberatos, G. 1992. Plasmid stabilization of an Escherichia coli culture through cycling. Biotechnol. Prog. 8: 1-4.
35. Walmsley, R., Gardner, D., Oliver, S. G. 1983. Stability of a cloned gene in yeast growth in chemostat culture. Mol. Gen. Genet. 192: 361-368.
36. Weber, A. E., San, K. Y. 1988. Enhanced plasmid maintenance in a CSTR upon square-wave oscillations in the dilution rate. Biotechnol. Lett. 10: 531-536.
37. Yang, S. T., Shu, C. H. 1996. Kinetics and stability of GM-CSF production by recombinant yeast cells immobilized in a fibrous-bed bioreactor. Biotechnol. Prog. 12: 449-456.
38. Zabriskie, D. W., Arcuri, E. J. 1986. Factors influencing productivity of fermentations employing recombinant microorganisms. Enzyme Microb. Technol. 8: 706-718.
39. Zabriskie, D. W. 1987. Effects of fermentation feeding strategies prior to induction of expression of a recombinant malaria antigen in E. coli. J. Industrial Microbiol. 2: 87-92.