Expression of the Fabs of human auto-antibodies in Escherichia coli: Optimization and determination of their fine binding characteristics and cross-reactivity

Journal of Molecular Biology

Volumn 308, Issue 3, 2001, Pages 527-539

  Kumar, Sanjeev ^a   Kalsi, Jatinderpal ^a   Latchman, David S ^b   Pearl, Laurence H ^a   Isenberg, David A ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Auto antibodies; Binding characteristics; Fab hybrids; Fab purification; Fab quantification

Indexed keywords

AUTOANTIBODY; CARDIOLIPIN ANTIBODY;

ANTIBODY SPECIFICITY; ANTIGEN BINDING; ANTIGEN SPECIFICITY; ARTICLE; BINDING AFFINITY; CROSS REACTION; DISEASE ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PATHOGENICITY; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; SYSTEMIC LUPUS ERYTHEMATOSUS;

ESCHERICHIA COLI;

EID: 0035804931     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4534     Document Type: Article

References (58)

1. Specific inhibition of the DNA-anti-DNA immune reaction by low molecular weight anionic compounds
2. Escherichia coli secretion of an active chimeric antibody fragment
3. Pairing of variable heavy and variable kappa chains in individual naive and memory B cells
4. The specificity of the anti-dsDNA ELISA. A closer look
5. Preparation and uses of Fab′ fragments from Escherichia coli
6. Crystallization and structure determination of an autoimmune anti poly(dT) immunoglobulin Fab fragment at 3.0 Å resolution
7. Single chain antibody (SCA) encoding genes: One-step construction and expression in eukaryotic cells
8. Analysis of heavy and light chain pairings indicates that receptor editing shapes the human antibody repertoire
9. Production and analysis of IgG monoclonal anti-DNA antibodies from systemic lupus erythematosus (SLE) patients
10. Serologic and genetic characterization of a human monoclonal immunoglobulin G anti-DNA idiotype
11. Human IgG anti-DNA antibodies deposit in kidneys and induce proteinuria in SCID mice
12. Crystallographic characterization of the Fab fragment of a monoclonal anti ss-DNA antibody
13. High-level production of a functional immunoglobulin heterodimer in a baculovirus expression system
14. An autoantibody to single-stranded DNA: Comparison of the three-dimensional structures of the unliganded Fab and a deoxynucleotide-Fab complex
15. Clustered arrangement of immunoglobulin lambda constant region genes in man
16. The importance of the light chain for the epitope specificity of human anti-Ul small nuclear RNA autoantibodies present in systemic lupus erythematsus patients
17. Light chain contribution to specificity in anti-DNA antibodies
18. The creation of diversity in the human immunoglobulin V(lambda) repertoire
19. Systemic lupus erythematosus - Adult onset
20. Long-term follow-up of autoantibody profiles in black female lupus patients and clinical comparison with Caucasian and Asian patients
21. Structural analysis of anti-DNA antibodies
22. Functional and modelling studies of the binding of human monoclonal anti-DNA antibodies to DNA
23. Analysis of kappa light chain contribution to anti-DNA antibody activity of a human VH4-21-encoded monoclonal antibody (NE-1) by antibody-phage display technique
24. Structural properties of a subset of nephritogenic anti-DNA antibodies
25. Poly-L-lysine causes false positive results in ELISA methods detecting anti-dsDNA antibodies
26. 3) larva of Necator americanus
27. Molecular cloning and expression of the Fabs of human auto-antibodies in Escherichia coli: Determination of the heavy or light chain contribution to the anti-DNA/-cardiolipin activity of the Fab
28. Lack of anti-DNA precursors in lambda chain-bearing B lymphocytes in (NZB × NZW)F1 mice. Evidence for the contribution of V kappa genes to anti-DNA specificity
29. The production, binding characteristics and sequence analysis of four human IgG monoclonal antiphospholipd antibodies
30. Amplification of chloramphenicol resistance transposons carried by pahge P1Cm in Escherichia coli
31. Structure of an immunoglobulin Fab fragment specific for triple-stranded DNA
32. Structure of an immunoglobulin Fab fragment specific for poly(dG)·poly(dC)
33. Significance of enzyme linked immunosorbant assay (ELISA) for antibodies to double stranded and single stranded DNA in patients with lupus nephritis: Correlation with severity of renal histology
34. The anti-DNA-associated idiotype 8.12 is encoded by the V lambda II gene family and maps to the vicinity of L chain CDR1
35. The 2.0-Å resolution crystal structure of a trimeric antibody fragment with noncognate VH-VL domain pairs shows a rearrangement of VH CDR3
36. Structural elements controlling anti-DNA antibody affinity and their relationship to anti-phosphorylcholine activity
37. Producing antibodies in Escherichia coli: From PCR to fermentation
38. Ig H and L chain contributions to autoimmune specificities
39. The importance of somatic mutations in the Vλ gene 2a2 in human monoclonal anti-DNA antibodies
40. Genetic, structural and functional properties of an IgG DNA-binding monoclonal antibody from a lupus patient with nephritis
41. Sm and DNA binding by dual reactive B cells requires distinct VH, V kappa, and VH CDR3 structures
42. Repertoire cloning of lupus anti-DNA autoantibodies
43. Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli
44. Production and properties of chimeric antibody molecules
45. Studies on the structure, regulation, and pathogenic potential of anti-dsDNA antibodies
46. Light chain usage in anti-double-stranded DNA B cell subsets: Role in cell fate determination
47. Bacterial expression of anti-DNA antibody domains
48. Cloning and functional analysis of anti-double strand DNA IgG autoantibodies using the phage-display method
49. Anti-dsDNA and complement profiles as prognostic guides in systemic lupus erythematosus
50. Human bifunctional antibody generated by heterologous association of heavy and light chains
51. Sequence and evolution of the human germline V lambda repertoire
52. IgG human monoclonal anti-DNA autoantibodies from patients with systemic lupus erythematosus
53. Influence of VH CDR3 arginine and light chain pairing on DNA reactivity of a bacterial DNA-induced anti-DNA antibody from a BALB/c mouse
54. Expression of proteins in prokaryotic systems - Principles and case studies