Roles for ligases in the RNA editing complex of Trypanosoma brucei: Band IV is needed for U-deletion and RNA repair

EMBO Journal

Volumn 20, Issue 17, 2001, Pages 4694-4703

  Huang, Catherine E ^a   Cruz Reyes, Jorge ^a   Zhelonkina, Alevtina G ^a   O'Hearn, Sean ^a   Wirtz, Elizabeth ^b   Sollner Webb, Barbara ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b ROCKEFELLER UNIVERSITY   (United States)

Author keywords

RNA editing; RNA ligase; Trypanosome; U deletion; U insertion

Indexed keywords

RNA; RNA LIGASE; URIDINE PHOSPHATE;

ARTICLE; CATALYSIS; CATALYST; CELL GROWTH; CELL LINE; CONTROLLED STUDY; ENZYME ACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN STABILITY; PROTEIN STRUCTURE; RNA EDITING; TRYPANOSOMA BRUCEI;

ANIMALS; KINETICS; MITOCHONDRIA; PLASMIDS; POLYMERASE CHAIN REACTION; POLYNUCLEOTIDE LIGASES; RECOMBINANT PROTEINS; RNA EDITING; RNA, MESSENGER; RNA, PROTOZOAN; SEQUENCE DELETION; TRANSFECTION; TRYPANOSOMA BRUCEI BRUCEI;

PHOCIDAE; PROTOZOA; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0035801635     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.17.4694     Document Type: Article

References (35)

1. The mechanism of U-insertion/deletion RNA editing in kinetoplastid mitochondria
2. Mechanism and evolution of RNA editing in kinetoplastida
3. A model for RNA editing in kinetoplastid mitochondria, 'guide' RNA molecules transcribed from maxicircle DNA provide the edited information
4. Uridine insertion/deletion RNA editing in Leishmania tarentolae mitochondria shows cell cycle dependence
5. Complexes from Trypanosoma brucei that exhibit deletion editing and other editing-associated properties
6. Trypanosome U-deletional RNA editing involves gRNA-directed endonuclease cleavage, terminal U-exonuclease and RNA ligase activities
7. T.brucei RNA editing, adenosine nucleotides inversely affect U-deletion and U-insertion reactions at mRNA cleavage
8. Trypanosoma brucei U-insertion and U-deletion activities co-purify with an enzymatic editing complex but are differentially optimized
9. Trypanosome RNA editing. Simple guide RNA features enhance U-deletion 100-fold
10. RNA editing involves indiscriminate U changes throughout precisely defined editing domains
11. Extensive editing of the cytochrome c oxidase III transcript in Trypanosoma bruce
12. Functions and mechanisms of RNA editing
13. Defining the editing 'reaction'
14. Effect of single amino acid changes in the region of the adenylylation site of T4 RNA ligase
15. Uridylate addition and RNA ligation contribute to the specificity of kinetoplastid insertion RNA editing
16. RNA editing, a mechanism for gRNA-specified uridylate insertion into precursor mRNA
17. Cycles of progressive realignment of gRNA with mRNA in RNA editing
18. The involvement of gRNA-binding protein gBP21 in RNA editing and in vitro and in vivo analysis
19. Identification of candidate mitochondrial RNA editing ligases from Trypanosoma brucei
20. Association of two novel proteins, TbMP52 and TbMP48, with the Trypanosoma brucei RNA editing complex
21. Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria
22. The two RNA ligases of the Trypanosoma brucei RNA editing complex, cloning the essential band IV gene and identifying the band V gene
23. RNA ligase and its involvement in guide RNA/mRNA chimera formation, evidence for a cleavage/ligation mechanism of Trypanosoma brucei mRNA editing
24. Biochemical methods for analysis of kinetoplastid RNA editing
25. An RNA ligase essential for RNA editing and survival of the bloodstream form of Trypanosoma brucei
26. RNA editing, transfer of genetic information from gRNA to precursor mRNA in vitro
27. Direct visualization of uridylate deletion in vitro suggests a mechanism for kinetoplastid RNA editing
28. Mutational analysis of vaccinia DNA ligase defines residues essential for covalent catalysis
29. RNA capping enzyme and DNA ligase superfamily of covalent nucleotidyl transferases
30. Unique ribonuclease activities of the trypanosome RNA editing complex can be directed to cleave specifically at a chosen site
31. RNA editing in kinetoplastid protozoa
32. Partially edited mRNAs for cytochrome b and subunit III of cytochrome oxidase from Leishmania tarentolae mitochondria, RNA editing intermediates
33. Location of the active site for enzyme-adenylate formation in DNA ligases
34. Regulated processive transcription of chromatin by T7 RNA polymerase in Trypanosoma brucei
35. A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in T. brucei