Uridylate addition and RNA ligation contribute to the specificity of kinetoplastid insertion RNA editing

Molecular and Cellular Biology

Volumn 20, Issue 22, 2000, Pages 8447-8457

  Igo R P , Jr ^a   Palazzo, S S ^a   Burgess, M L K ^a   Panigrahi, A K ^a   Stuart, K ^a  

^a SEATTLE BIOMEDICAL RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA; URIDINE PHOSPHATE;

ARTICLE; KINETOPLAST; NONHUMAN; PRIORITY JOURNAL; RNA ANALYSIS; RNA EDITING; RNA STRUCTURE; TRYPANOSOMA BRUCEI;

ANIMALS; BASE SEQUENCE; DNA TRANSPOSABLE ELEMENTS; GENETIC TECHNIQUES; KINETOPLASTIDA; MOLECULAR SEQUENCE DATA; RNA EDITING; RNA, MESSENGER; TIME FACTORS; URIDINE;

EID: 0033762432     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.20.22.8447-8457.2000     Document Type: Article

References (27)

1. The mechanism of U insertion/deletion RNA editing in kinetoplastid mitochondria
2. The association of guide RNA binding protein gBP21 with active RNA editing complexes in Trypanosoma brucei
3. The Leishmania kinetoplast-mitochondrion contains terminal uridylyltransferase and RNA ligase activities
4. An extensively edited mitochondrial transcript in kinetoplastids encodes a protein homologous to ATPase subunit 6
5. The mitochondrial RNA ligase from Leishmania tarentolae can join RNA molecules bridged by a complementary RNA
6. A model for RNA editing in kinetoplastid mitochondria: 'Guide' RNA molecules transcribed from maxicircle DNA provide the edited information
7. Guide RNAs in kinetoplastid mitochondria have a nonencoded 3' oligo(U) tail involved in recognition of the preedited region
8. Kinetoplastid RNA editing does not require the terminal 3' hydroxyl of guide RNA, but modifications to the guide RNA terminus can inhibit in vitro U insertion
9. Guide RNA-directed uridine insertion RNA editing in vitro
10. Complexes from Trypanosoma brucei that exhibit deletion editing and other editing-associated properties
11. T. brucei RNA editing: Adenosine nucleotides inversely affect U-deletion and U-insertion reactions at mRNA cleavage
12. Trypanosoma brucei U insertion and U deletion activities co-purify with an enzymatic editing complex but are differentially optimized
13. Trypanosome U-deletional RNA editing involves guide RNA-directed endonuclease cleavage, terminal U exonuclease, and RNA ligase activities
14. Addition of uridines to edited RNAs in trypanosome mitochondria occurs independently of transcription
15. RNA editing: A mechanism for gRNA-specified uridylate insertion into precursor mRNA
16. In vitro uridine insertion RNA editing mediated by cis-acting guide RNAs
17. Guide RNAs for transcripts with developmentally regulated RNA editing are present in both life cycle stages of Trypanosoma brucei
18. Trypanosoma brucei guide RNA poly(U) tail formation is stabilized by cognate mRNA
19. Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates
20. Editing domains of Trypanosoma brucei mitochondrial RNAs identified by secondary structure
21. Native mRNA editing complexes from Trypanosoma brucei mitochondria
22. Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria
23. Guide RNA-mRNA chimeras, which are potential RNA editing intermediates, are formed by endonuclease and RNA ligase in a trypanosome mitochondrial extract
24. RNA ligase and its involvement in guide RNA/mRNA chimera formation
25. Direct visualization of uridylate deletion in vitro suggests a mechanism for kinetoplastid RNA editing
26. RNA editing in kinetoplastid protozoa
27. Investigating the mechanism and machinery of RNA editing