Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria

EMBO Journal

Volumn 16, Issue 13, 1997, Pages 4069-4081

  Rusché, Laura N ^a   Cruz Reyes, Jorge ^a   Piller, Kenneth J ^a   Sollner Webb, Barbara ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Editing complex; RNA editing; RNA lipase; Trypanosoma brucei; Trypanosome

Indexed keywords

ENDONUCLEASE; GUIDE RNA; HEXOSE 1 PHOSPHATE URIDYLYLTRANSFERASE; MITOCHONDRIAL RNA; RNA HELICASE;

ARTICLE; LIGATION; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; RNA EDITING; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

ANIMALS; CELL FRACTIONATION; CELLULOSE; DNA; ENDORIBONUCLEASES; EXONUCLEASES; MITOCHONDRIA; MULTIENZYME COMPLEXES; RNA EDITING; RNA LIGASE (ATP); RNA NUCLEOTIDYLTRANSFERASES; RNA, PROTOZOAN; TRYPANOSOMA BRUCEI BRUCEI;

EID: 0030928201     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.13.4069     Document Type: Article

References (36)

1. Arts, G.J. and Benne, R. (1996) Mechanism and evolution of RNA editing in kinetoplastids. Biochim. Biophys. Acta, 1307, 39-59.
2. Blum, B. and Simpson, L. (1990) Guide RNAs in kinetoplastid mitochondria have a nonencoded 3′ oligo(U) tail involved in recognition of the preedited region. Cell, 62, 391-397.
3. Blum, B., Bakalara, N. and Simpson, L. (1990) A model for RNA editing in kinetoplastid mitochondria: 'guide' RNA molecules transcribed from maxicircle DNA provide the edited information. Cell, 60, 189-198.
4. Blum, B., Sturm, N.R., Simpson, A.M. and Simpson, L. (1991) Chimeric gRNA-mRNA molecules with oligo(U) tails covalently linked at sites of RNA editing suggest that U addition occurs by transesterification. Cell, 65, 543-550.
5. Bringaud, F., Peris, M., Zen, K.H. and Simpson, L. (1995) Characterization of two nuclear-encoded protein components of mitochondrial ribonucleoprotein complexes from Leishmania tarentolae. Mol. Biochem. Parasitol., 71, 65-79.
6. Byrne, E., Bringaud, F. and Simpson, L. (1995) RNA-protein interactions in the ribonucleoprotein T-complexes in a mitochondrial extract from Leishmania tarentolae. Mol. Biochem. Parasitol., 72, 65-76.
7. Byrne, E.M., Connell, G.J. and Simpson, L. (1996) Guide RNA-directed uridine-insertion RNA editing in vitro. EMBO J., 15, 6758-6765.
8. Cech, T.R. (1991) RNA editing: World's smallest intron? Cell, 64, 667-669.
9. Corell, R.A. et al. (1996) Complexes from Trypanosoma brucei that exhibit deletion editing and other editing-associated properties. Mol. Cell. Biol., 16, 1410-1418.
10. Cruz-Reyes, J. and Sollner-Webb, B. (1996) Trypanosome U-deletional RNA editing involves gRNA-directed endonuclease cleavage, terminal U-exonuclease, and RNA ligase activities. Proc. Natl Acad. Sci. USA, 93, 8901-8906.
11. Decker, C.J. and Sollner-Webb, B. (1990) RNA editing involves indiscriminate U changes throughout precisely defined editing domains. Cell, 61, 1001-1011.
12. Frech, G.C., Bakalara, N., Simpson, L. and Simpson, A.M. (1995) In vitro RNA editing-like activity in a mitochondrial extract from Leishmania tarentolae. EMBO J., 14, 178-187.
13. Göringer, H.U., Koslowsky, D.J., Morales, T.H. and Stuart, K. (1994) The formation of mitochondrial ribonucleoprotein complexes involving guide RNA molecules in Trypanosoma brucei. Proc. Natl Acad. Sci. USA, 91, 1776-1780.
14. Göringer, H.U., Köller, J. and Shu, H.H. (1995) Multicomponent complexes involved in kinetoplastid RNA editing. Parasitol. Today, 11, 265-267.
15. Harris, M., Decker, C., Sollner-Webb, B. and Hajduk, S. (1992) Specific cleavage of pre-edited mRNAs in trypanosome mitochondrial extracts. Mol. Cell. Biol., 12, 2591-2598.
16. Kable, M.L., Seiwert, S.D., Heidmann, S. and Stuart, K. (1996) RNA editing: a mechanism for gRNA-specified uridylate insertion into precursor mRNA. Science, 273, 1189-1195.
17. Köller, J., Nörskau, G., Paul, A.S., Stuart, K. and Göringer, H.U. (1994) Different Trypanosoma brucei guide RNA molecules associate with an identical complement of mitochondrial proteins in vitro. Nucleic Acids Res., 22, 1988-1995.
18. Leegwater, P., Speijer, D. and Benne, R. (1995) Identification by UV cross-linking of oligo(U)-binding proteins in mitochondria of the insect trypanosomatid Crithidia fasciculata. Eur. J. Biochem., 227, 780-786.
19. Missel, A. and Göringer, H.U. (1994) Trypanosoma brucei mitochondria contain RNA helicase activity. Nucleic Acids Res., 22, 4050-4056.
20. Peris, M., Frech, G.C., Simpson, A.M., Bringaud, F., Byrne, E., Bakker, A. and Simpson, L. (1994) Characterization of two classes of ribonucleoprotein complexes possibly involved in RNA editing from Leishmania tarentolae mitochondria. EMBO J., 13, 1664-1672.
21. Piller, K.J., Decker, C.J., Rusché, L.N., Harris, M.E., Hajduk, S.L. and Sollner-Webb, B. (1995a) Editing domains of Trypanosoma brucei mitochondrial RNAs identified by secondary structure. Mol. Cell. Biol., 15, 2916-2924.
22. Piller, K.J., Decker, C.J., Rusché, L.N. and Sollner-Webb, B. (1995b) Trypanosoma brucei mitochondrial guide RNA-mRNA chimeraforming activity cofractionates with an editing-domain specific endonuclease and RNA ligase and is mimicked by heterologous nuclease and RNA ligase. Mol. Cell. Biol., 15, 2925-2932
23. Piller, K.J., Rusché, L.N. and Sollner-Webb, B. (1996) Trypanosoma brucei RNA editing: a full round of uridylate insertional editing in vitro mediated by endonuclease and RNA ligase. J. Biol. Chem., 271, 4613-4619.
24. Piller, K.J., Rusché, L.N., Cruz-Reyes, J. and Sollner-Webb, B. (1997) Resolution of the RNA editing gRNA-directed endonuclease from two other endonucleases of T. brucei mitochondria. RNA, 3, 279-290.
25. Pollard, V.W., Rohrer, S.P. Michelotti, E.F., Hancock, K. and Hajduk, S.L. (1990) Organization of minicircle genes for guide RNAs in Trypanosoma brucei. Cell, 63, 783-790.
26. Pollard, V.W., Harris, M.E. and Hajduk, S.L. (1992) Native mRNA editing complexes from Trypanosoma brucei mitochondria. EMBO J., 11, 4429-1438.
27. Read, L.K., Göringer, H.U. and Stuart, K. (1994) Assembly of mitochondrial ribonucleoprotein complexes involves specific guide RNA (gRNA)-binding proteins and gRNA domains but does not require pre-edited mRNA. Mol. Cell. Biol., 14, 2629-2639.
28. Riley, G.R., Corell, R.A. and Stuart, K. (1994) Multiple guide RNAs for identical editing of Trypanosoma brucei apocytochrome β mRNA have an unusual minicircle location and are developmentally regulated. J. Biol. Chem., 269, 6101-6108.
29. Rusché, L.N., Piller, K.J. and Sollner-Webb, B. (1995) Guide RNA-mRNA chimeras, which are potential RNA editing intermediates, are formed by endonuclease and RNA ligase in a trypanosome mitochondrial extract. Mol. Cell. Biol., 15, 2933-2941.
30. Sabatini, R. and Hajduk, S.L. (1995) RNA ligase and its involvement in guide RNA/mRNA chimera formation: evidence for a cleavage-ligation mechanism of Trypanosoma brucei mRNA editing. J. Biol. Chem., 270, 7233-7240.
31. Seiwert, S.D. and Stuart, K. (1994) RNA editing: transfer of genetic information from gRNA to precursor mRNA in vitro. Science, 266, 114-117.
32. Seiwert, S.D., Heidmann, S. and Stuart, K. (1996) Direct visualization of uridylate deletion in vitro suggests a mechanism for kinetoplastid RNA editing. Cell, 84, 831-841.
33. Sharp, P. (1994) Split genes and RNA splicing. Cell, 77, 805-815.
34. Shu, H.H., Stuart, K. and Göringer, H.U. (1995) Guide RNA molecules not engaged in RNA editing form ribonucleoprotein complexes tree of mRNA. Biochim. Biophys. Acta, 1261, 349-359.
35. Simpson, A.M., Bakalara, N. and Simpson, L. (1992) A ribonuclease activity is activated by heparin or by digestion with proteinase K in mitochondrial extracts of Leishmania tarentolae. J. Biol. Chem., 267, 6782-6788.
36. Sollner-Webb, B. (1991) RNA editing. Curr. Opin. Cell Biol., 3, 1056-1061.