Solid-state NMR data support a helix-loop-helix structural model for the N-terminal half of HIV-1 Rev in fibrillar form

Journal of Molecular Biology

Volumn 313, Issue 4, 2001, Pages 845-859

  Blanco, Francisco J ^a   Hess, Sonja ^a   Pannell, Lewis K ^a   Rizzo, Nancy W ^b   Tycko, Robert ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

HIV; Protein fibrils; Rev protein; Solid state NMR; Structure determination

Indexed keywords

HELIX LOOP HELIX PROTEIN; REV PROTEIN; VIRUS PROTEIN;

AMINO TERMINAL SEQUENCE; ANISOTROPY; ARTICLE; BINDING KINETICS; CIRCULAR DICHROISM; CONTROLLED STUDY; CRYSTALLIZATION; DNA RESPONSIVE ELEMENT; ENZYME BINDING; ENZYME STRUCTURE; FIBER; GENETIC CODE; GENETIC TRANSCRIPTION; HOST CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; LIQUID CRYSTAL; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN MOTIF; PROTEIN SECONDARY STRUCTURE; QUANTUM MECHANICS; SOLID STATE; STRUCTURE ANALYSIS; VIRUS REPLICATION;

EID: 0035798396     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.5067     Document Type: Article

References (78)

1. A second posttranscriptional transactivator gene required for HTLV-III replication
2. HTLV-III Expression and production involve complex regulation at the levels of splicing and translation of viral-RNA
3. HIV-1: Fifteen proteins and an RNA
4. Structural biology of HIV
5. Cis-acting sequences responsive to the Rev gene-product of the human immunodeficiency virus
6. Specific binding of HIV-1 recombinant rev protein to the Rev-responsive element in vitro
7. A cis-acting repressive sequence that overlaps the Rev-responsive element of human-immunodeficiency-virus type-1 regulates nuclear retention of Env messenger-RNAs independently of known splice signals
8. Effector domains of human-immunodeficiency-virus type-1 Rev and human T-cell leukemia-virus type-I Rex are functionally interchangeable and share an essential peptide motif
9. The ins and outs of HIV Rev
10. Tolerance of diverse amino acid substitutions at conserved positions in the nuclear export signal (NES) of HIV-1 Rev
11. The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export
12. Posttranscriptional regulation by the HIV-1 Rev protein
13. HIV-1 regulator of virion expression (Rev) protein binds to an RNA stem-loop structure located within the Rev response element region
14. Characterization of HIV-1 Rev protein: Binding stoichiometry and minimal RNA substrate
15. Structural analysis of the interaction between the human-immunodeficiency-virus rev protein and the rev response element
16. Real-time kinetics of HIV-1 Rev-Rev response element interactions: Definition of minimal, binding sites on RNA and protein and stoichiometric analysis
17. Solution oligomerization of the Rev protein of HIV-1: Implications for function
18. Biochemical characterization of binding of multiple HIV-1 Rev monomeric proteins to the Rev responsive element
19. Oligomerization and RNA-binding domains of the type-1 human-immunodeficiency-virus Rev protein: A dual function for an arginine-rich binding motif
20. Sensitive in vitro analysis of HIV-1 Rev multimerization
21. Specific regulation of messenger-RNA splicing in vitro by a peptide from HIV-1 Rev
22. Transdominant inhibition of human-immunodeficiency-virus typed-1 Rev occurs through formation of inactive protein complexes
23. Functional analysis of the human immunodeficiency virus type 1 Rev protein oligomerization interface
24. Specific binding of a basic peptide from HIV-1 Rev
25. The basic domain of Rev from human-immunodeficiency-virus type-1 specifically blocks the entry of U4/U6 U5 small nuclear ribonucleoprotein in spliceosome assembly
26. RNA recognition by an isolated alpha-helix
27. Costabilization of peptide and RNA structure in an HIV Rev peptide-RRE complex
28. NMR solution structure of the RNA-binding peptide from human- immunodeficiency-virus (type-1) Rev
29. Mechanism of neomycin and Rev peptide binding to the Rev responsive element of HIV-1 as determined by fluorescence and NMR spectroscopy
30. 1H NMR studies of the high-affinity Rev binding-site of the Rev responsive element of HIV-1 messenger-RNA: Base pairing in the core binding element
31. Binding of an HIV Rev peptide to Rev responsive element RNA induces formation of purine-purine base-pairs
32. Structural change in Rev responsive element RNA of HIV-1 on binding Rev peptide
33. 13C heteronuclear NMR
34. Alpha helix-RNA major groove recognition in an HIV-1 Rev peptide RRE RNA complex
35. Deep penetration of an α-helix into a widened RNA major groove in the HIV-1 rev peptide-RNA aptamer complex
36. Peptide-triggered conformational switch in HIV-1 RRE RNA complexes
37. The crystal structure of the Rev binding element of HIV-1 reveals novel base pairing and conformational variability
38. The structure of the HIV-1 RRE high affinity Rev binding site at 1.6 Ångstrom resolution
39. HIV-1 Rev expressed in recombinant Escherichia coli: Purification, polymerization, and conformational properties
40. Three-dimensional structure of HIV-1 Rev protein filaments
41. Circular dichroism studies of the HIV-1 Rev protein and its specific RNA binding site
42. Helix-loop-helix motif in HIV-1 Rev
43. Probing the structure of the HIV-1 Rev trans-activator protein by functional analysis
44. Intermolecular binding sites of human-immunodeficiency-virus type-1 Rev protein determined by protein footprinting
45. Probing the structure of HIV-1 Rev by protein footprinting of multiple monoclonal antibody-binding sites
46. Quantitative conformational measurements in solid-state NMR by constant-time homonuclear dipolar recoupling
47. 13C chemical shift anisotropy measurements
48. Determination of the helix and beta form of proteins in aqueous solution by circular dichroism
49. Association-induced folding of globular proteins
50. CD spectroscopy and the helix-coil transition in peptides and polypeptides
51. Formation of insulin amyloid fibrils followed by FTIR simultaneously with CD and electron microscopy
52. 16-22, a seven-residue fragment of the Alzheimer's β-amyloid peptide, and structural characterization by solid-state NMR
53. Solid state NMR evidence for an antibody-dependent conformation of the V3 loop of HIV-1 gp120
54. Helix geometry in proteins
55. 10-helix contents of a helical peptide
56. A simple magic angle spinning NMR experiment for the dephasing of rotational echoes of dipolar coupled homonuclear spin pairs
57. Homonuclear radio-frequency-driven recoupling in rotating solids
58. 15N NMR assignment and solution structure of the SH3 domain of spectrin: Comparison of unrefined and refined structure sets with the crystal structure
59. Crystal structure of a SRC-homology-3 (SH3) domain
60. Prospects for resonance assignments in multidimensional solid-state NMR spectra of uniformly labeled proteins
61. Probing hydrogen bonds in the antibody-bound HIV-1 gp120 V3 loop by solid-state NMR REDOR measurements
62. Sample optimization and identification of signal patterns of amino acid side chains in 2D RFDR spectra of the alpha-spectrin SH3 domain
63. 15N)-enriched BPTI in the solid-state
64. Determination of peptide conformations by two-dimensional magic angle spinning NMR exchange spectroscopy with rotor synchronization
65. Investigation of molecular structure in solids by two- dimensional NMR exchange spectroscopy with magic angle spinning
66. Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeast
67. Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins
68. Nuclear export of late HIV-1 mRNAs occurs via a cellular protein export pathway
69. HIV Rev uses a conserved cellular protein export pathway for the nucleocytoplasmic transport of viral RNAs
70. The HIV-1 Rev protein
71. Scanning mutagenesis of the arginine-rich region of the human-immunodeficiency-virus type-1 Rev trans activator
72. Importin beta can mediate the nuclear import of an arginine-rich nuclear localization signal in the absence of importin alpha
73. An efficient and cost-effective isotope labeling protocol for proteins expressed in Escherichia coli
74. Determination of D-amino acids. 2. Use of a bifunctional reagent, 1,4,-difluoro-2,4,-dinitrobenzene
75. 15N]alanine from the dipole-coupled powder patterns
76. 15N dipole-coupled chemical shift powder patterns
77. 14N electric field gradient tensor orientations with respect to the molecular frame in a polypeptide
78. Sideband intensities in NMR spectra of samples spinning at the magic angle