Evaluation of the tetracycline- and ecdysone-inducible systems for expression of neurotransmitter receptors in mammalian cells

European Journal of Neuroscience

Volumn 14, Issue 6, 2001, Pages 968-976

  Van Craenenbroeck, Kathleen ^a   Vanhoenacker, Peter ^a   Leysen, Josée E ^b   Haegeman, Guy ^a  

^a GHENT UNIVERSITY   (Belgium)

^b JANSSEN RESEARCH FOUNDATION   (Belgium)

Author keywords

Ecdysone inducible expression system; Inducible expression; Serotonin receptor; Tetracycline inducible expression system

Indexed keywords

CHLORAMPHENICOL ACETYLTRANSFERASE; ECDYSONE; G PROTEIN COUPLED RECEPTOR; LIGAND; NEUROTRANSMITTER RECEPTOR; SEROTONIN 1B RECEPTOR; SEROTONIN 1F RECEPTOR; SEROTONIN 4 RECEPTOR; SEROTONIN RECEPTOR; TETRACYCLINE; TRANSACTIVATOR PROTEIN;

ANIMAL CELL; ARTICLE; CELL LINE; CELL STRAIN L 929; CONTINUOUS CULTURE; CONTROLLED STUDY; EXPRESSION VECTOR; HUMAN; HUMAN CELL; LIGAND BINDING; MAMMAL CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN INDUCTION; QUANTITATIVE ANALYSIS; REGULATORY MECHANISM;

ANIMALS; ANTI-BACTERIAL AGENTS; BLOTTING, NORTHERN; BLOTTING, WESTERN; CELLS, CULTURED; CHLORAMPHENICOL O-ACETYLTRANSFERASE; ECDYSONE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GTP-BINDING PROTEINS; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); MICE; NERVE TISSUE PROTEINS; PLASMIDS; POLYMERASE CHAIN REACTION; RECEPTOR, SEROTONIN, 5-HT1B; RECEPTORS, NEUROTRANSMITTER; RECEPTORS, SEROTONIN; RECEPTORS, SEROTONIN, 5-HT4; TETRACYCLINE; TRANSFECTION;

EID: 0035783471     PISSN: 0953816X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0953-816X.2001.01719.x     Document Type: Article

References (46)

1. Amin, A.R., Attur, M.G., Thakker, G.D., Patel, P.D., Vyas, P.R., Patel, R.N., Patel, I.R. & Abramson, S.B. (1996) A novel mechanism of action of tetracyclines: Effects on nitric oxide synthases. Proc. Natl. Acad. Sci. USA, 93, 14014-14019.
2. Attur, M.G., Patel, R.N., Patel, P.D., Abramson, S.S. & Amin, A.R. (1999) Tetracycline up-regulates COX-2 expression and prostaglandin E-2 production independent of its effect on nitric oxide. J. Immunol., 162, 3160-3167.
3. 4 receptor mRNA and ligand binding sites in the post-mortem human brain. Synapse, 36, 35-46.
4. Christopherson, K.S., Mark, M.R., Bajaj, V. & Godowski, P.J. (1992) Ecdysteroid-dependent regulation of genes in mammalian cells by a Drosophila ecdysone receptor and chimeric transactivators. Proc. Natl. Acad. Sci. USA, 89, 6314-6318.
5. Corti, O., Sanchez-Capelo, A., Colin, P., Hanoun, N., Hamon, M. & Mallet, J. (1999) Long-term doxycycline-controlled expression of human tyrosine hydroxylase after direct adenovirus-mediated gene transfer to a rat model of Parkinson's disease. Proc. Natl. Acad. Sci. USA, 96, 12120-12125.
6. Efrat, S., Fusco DeMane, D., Lemberg, H., al Emran, O. & Wang, X. (1995) Conditional transformation of a pancreatic beta-cell line derived from transgenic mice expressing a tetracycline-regulated oncogene. Proc. Natl. Acad. Sci. USA, 92, 3576-3580.
7. Fishmann, G.I., Kaplan, M.L. & Buttrick, P.M. (1994) Tetracycline-regulated cardiac gene expression in vivo. J. Clin. Invest., 93, 1864-1868.
8. Freundlieb, S., Baron, U., Bonin, A.L., Gossen, M. & Bujard, H. (1997) Use of tetracycline-controlled gene expression systems to study mammalian cell cycle. Meth. Enzymol., 283, 159-173.
9. Freundlieb, S., Schirra-Muller, C. & Bujard, H. (1999) A tetracycline controlled activation/repression system with increased potential for gene transfer into mammalian cells. J. Gen. Med., 1, 4-12.
10. Furth, P.A., St Onge, L., Boger, H., Gruss, P., Gossen, M., Kistner, A., Bujard, H. & Hennighausen, L. (1994) Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc. Natl. Acad. Sci. USA, 91, 9302-9306.
11. Girard, F., Bello, B. & Laemmli, U.K. & Gehring, W.J. (1998) In vivo analysis of scaffold-associated regions in Drosophila: A synthetic high-affinity SAR binding protein supresses position effect variegation. EMBO J., 17, 2079- 2085.
12. Gossen, M., Freundlieb, S., Bender, G., Muller, G., Hillen, W. & Bujard, H. (1995) Transcriptional activation by tetracyclines in mammalian cells. Science, 268, 1766-1769.
13. Gregory, P.D. & Hortz, W. (1998) Life with nucleosomes: Chromatin remodeling in gene regulation. Curr. Opin. Cell Biol., 10, 339-345.
14. Howe, J.R., Skryabin, B.V., Belcher, S.M., Zerillo, C.A. & Schmauss, C. (1995) The responsiveness of a tetracycline-sensitive expression system differs in different cell lines. J. Biol. Chem., 270, 14168-14174.
15. Kistner, A., Gossen, M., Zimmermann, F., Jerecic, J., Ullmer, C., Lubbert, H. & Bujard, H. (1996) Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc. Natl. Acad. Sci. USA, 93, 10933-10938.
16. 1D receptors expressed in various mammalian cell lines. Br. J. Pharmacol., 123, 1655-1665.
17. 3H]alniditan. Mol. Pharmacol., 50, 1567-1580.
18. Mayford, M., Bach, M.E., Huang, Y.Y., Wang, L., Hawkins, R.D. & Kandel, E.R. (1996) Control of memory formation through regulated expression of a CaMKII transgene. Science, 274, 1678-1683.
19. Mazur, X., Eppenberger, H.M., Bailey, J.E. & Fussenegger, M. (1999) A novel autoregulated proliferation-controlled production process using recombinant CHO cells. Biotechnol. Bioeng., 65, 144-150.
20. No, D., Yao, T.P. & Evans, R.M. (1996) Ecdysone-inducible gene expression in mammalian cells and transgenic mice. Proc. Natl. Acad. Sci. USA, 93, 3346-3351.
21. Paulus, W., Baur, I., Boyce, F.M., Breakefield, X.O. & Reeves, S.A. (1996) Self-contained, tetracycline-regulated retroviral vector system for gene delivery to mammalian cells. J. Virol., 70, 62-67.
22. Pillutla, R.C., Shimamoto, A., Furuichi, Y. & Shatkin, A.J. (1999) Genomic structure and chromosomal localization of TCEAL1, a human gene encoding the nuclear phosphoprotein p21/SIIR. Genomics, 56, 217-220.
23. Ralph, G.S., Bienemann, A., Harding, T.C., Hopton, M., Henley, J. & Uney, J.B. (2000) Targeting of tetracycline-regulatable transgene expression specifically to neuronal and glial cell populations using adenoviral vectors. Neuroreport, 11, 2051-2055.
24. Rang, A. & Will, H. (2000) The tetracycline-responsive promoter contains functional interferon-inducible response elements. Nucl. Acids Res., 28, 1120-1125.
25. Roder, F.T., Schmulling, T. & Gatz, C. (1994) Efficiency of the tetracycline-dependent gene expression system: Complete suppression and efficient induction of the rolB phenotype in transgenic plants. Mol. Gen. Genet., 243, 32-38.
26. Rossi, F.M.V., Guicherit, O.M., Spicher, A., Kringstein, A.M., Fatyol, K., Blakely, B.T. & Blau, H.M. (1998) Tetracycline-regulatable factors with distinct dimerization domains allow reversible growth inhibition by p16. Nature Genet., 20, 389-393.
27. Shockett, P., Difilippantonio, M., Hellman, N. & Schatz, D.G. (1995) A modified tetracycline-regulated system provides autoregulatory, inducible gene expression in cultured cells and transgenic mice. Proc. Natl. Acad. Sci. USA, 92, 6522-6526.
28. Soulier, S., Stinnakre, M.G., Lepourry, L., Mercier, J.C. & Vilotte, J.L. (1999) Use of doxycycline-controlled gene expression to reversibly alter milk-protein composition in transgenic mice. Eur. J. Biochem., 260, 533-539.
29. Suhr, S., Gil, E., Senut, M. & Gage, F. (1998) High level transactivation by a modified bombyx ecdysone receptor in mammalian cells without exogenous retinoid X receptor. Proc. Natl. Acad. Sci. USA, 95, 7999-8004.
30. Sun, C.H. & Tai, J.H. (2000) Development of a tetracycline controlled gene expression system in the parasitic protozoan Giardia lamblia. Mol. Biochem. Parasitol., 105, 51-60.
31. Tesson, L., Charreau, B., Menoret, S., Gilbert, E., Soulillou, J.P. & Anegon, I. (1999) Endothelial expression of Fas ligand in transgenic rats under the temporal control of a tetracycline-inducible system. Transplant. Proc., 31, 1533-1534.
32. Thomas, H.E., Stunnenberg, H.G. & Stewart, A.F. (1993) Heterodimerization of the Drosophila ecdysone receptor with retinoid X receptor and ultraspiracle. Nature, 362, 471-475.
33. Van Craenenbroeck, K., Vanhoenacker, P., Duchau, H. & Haegeman, G. (2000) Molecular integrity and usefulness of episomal expression vectors derived from BK and Epstein-Barr virus. Gene, 253, 293-301.
34. 4 receptor cDNA. J. Neurochem., 69, 1810-1819.
35. Vanden Berghe, W., Plaisance, S., Boone, E., DeBosscher, K., Schmitz, M., Fiers, W. & Haegeman, G. (1998) p38 and extracellular signal-regulated kinase mitogen-activated protein kinase pathways are required for nuclear factor-kappa b p65 transactivation mediated by tumor necrosis factor. J. Biol. Chem., 273, 3285-3290.
36. Vandevoorde, V., Haegeman, G. & Fiers, W. (1997) Induced expression of trimerized intracellular domains of the human tumor necrosis factor (TNF) p55 receptor elicits TNF effects. J. Cell. Biol., 137, 1627-1638.
37. Vanhoenacker, P., Fiers, W. & Haegeman, G. (1994) Studies on the induction of the interleukin-6 promoter in cell lines of human and simian origin. Eur. Cytokine Netw., 5, 283-291.
38. Vanhoenacker, P., Gommeren, W., Luyten, W.H.M.L., Leysen, J.E. & Haegeman, G. (1997) Stable, high-level expression of human serotonin receptors in L929 cells using an inducible expression system. Recept. Channels, 5, 125-137.
39. Vanhoenacker, P., Gommeren, W., Luyten, W.H.M.L., Leysen, J.E. & Haegeman, G. (1999) Optimized expression of serotonin receptors in mammalian cells using inducible expression systems. Gene Ther. Mol. Biol., 3, 301-310.
40. Weinmann, P., Gossen, M., Hillen, W., Bujard, H. & Gatz, C. (1994) A chimeric transactivator allows tetracycline-responsive gene expression in whole plants. Plant J., 5, 559-569.
41. Wells, K.D., Foster, J.A., Moore, K., Pursel, V.G. & Wall, R.J. (1999) Codon optimization, genetic insulation, and an rtTA reporter improve performance of the tetracycline switch. Transgenic Res., 8, 371-381.
42. Yao, T.P., Forman, B.M., Jiang, Z., Cherbas, L., Chen, J.D., McKeown, M., Cherbas, P. & Evans, R.M. (1993) Functional ecdysone receptor is the product of EcR and ultraspiracle genes. Nature, 366, 476-479.
43. Yao, T.P., Segraves, W.A., Oro, A.E., McKeown, M. & Evans, R.M. (1992) Drosophila ultraspiracle modulates ecdysone receptor function via heterodimer formation. Cell, 71, 63-72.
44. Yates, J.L., Warren, N. & Sugden, B. (1985) Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells. Nature, 313, 812-815.
45. Yeh, C.H. & Shatkin, A.J. (1994) Down-regulation of Rous sarcoma virus long terminal repeat promoter activity by a HeLa cell basic protein. Proc. Natl. Acad. Sci. USA, 91, 11002-11006.
46. Zhang, Y., Ochalski, R., Wardwell-Swanson, J. & Dunlop, J. (2000) Ecdysone inducible expression poorly regulates human 5-HT2A receptor gene expression in CHO cells. Soc. Neurosci. Abstr., 26, 273.