Genomic structure of proton-coupled oligopeptide transporter hPEPT1 and pH-sensing regulatory splice variant

AAPS PharmSci

Volumn 3, Issue 1, 2001, Pages

  Urtti, Arto ^a,b   Johns, Susan Jean ^a   Sadee, Wolfgang ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF EASTERN FINLAND   (Finland)

Author keywords

Genomic structure; Peptide transporter; Splice variant

Indexed keywords

BETA LACTAM ANTIBIOTIC; CARRIER PROTEIN; COTRANSPORTER; INSULIN; OLIGOPEPTIDE; PROTEIN HPEPT1; UNCLASSIFIED DRUG; ISOPROTEIN; PEPT1 PROTEIN;

ARTICLE; CELL MEMBRANE TRANSPORT; CHROMOSOME 13; CONTROLLED STUDY; DATA BASE; DRUG BIOAVAILABILITY; DRUG TRANSPORT; ENDOTHELIUM CELL; EPITHELIUM CELL; EXON; GC RICH SEQUENCE; GENE LOCATION; GENE SEQUENCE; GENE STRUCTURE; GENETIC ANALYSIS; GENETIC CODE; GENETIC ORGANIZATION; HUMAN; INSULIN RESPONSE; INTRON; MEMBRANE POTENTIAL; NUCLEOTIDE SEQUENCE; ORTHOLOGY; PH; PROMOTER REGION; PROTEIN TRANSPORT; PROTON TRANSPORT; REMOTE SENSING; SENSITIVITY ANALYSIS; SPLICEOSOME; TATA BOX; ALTERNATIVE RNA SPLICING; AMINO ACID SEQUENCE; ANIMAL; GENETICS; MOLECULAR GENETICS; MOUSE;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMAL; BASE SEQUENCE; CARRIER PROTEINS; CHROMOSOMES, HUMAN, PAIR 13; HUMAN; HYDROGEN-ION CONCENTRATION; MICE; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); PROTEIN ISOFORMS; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0035783146     PISSN: 15221059     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Sadee W, Druebbisch V, Amidon GL. Biology of membrane transport proteins. Pharm Res. 1995;12:1823-1837.
2. Fei YJ, Kanai Y, Nussberger S, et al. Expression cloning of a mammalian proton-coupled oligopeptide transporter. Nature. 1994;368:563-566.
3. Liang R, Fei YJ, Prasad PD, et al. Human intestinal H+/peptide cotransporter: cloning, functional expression, and chromosomal localization. J Biol Chem. 1995;270:6456-6463.
4. Liu W, Liang R, Ramamoorthy S, et al. Molecular cloning of PEPT2, a new member of the H+/peptide cotransporter family, from human kidney. Biochem Biophys Acta 1995;1235:461-466.
5. Yamashita T, Shimada S, Guo W, et al. Cloning and functional expression of a brain peptide/histidine transporter. J Biol Chem. 1997;272:10205-10211.
6. Botka C, Wittig TW, Graul RC, et al. Human proton/oligopeptide transporter (POT) genes: identification of putative human genes using bioinformatics. AAPS PharmSci. 2000;2: (2), article 16. Available from:http://www.pharmsci.org/scientificjournals/pharmsci/journal/16.html
7. Covitz KMY, Amidon GL, Sadee W. Membrane topology of the human dipeptide transporter, hPEPT1, determined by epitope insertions. Biochem. 1998;37:15214-15221.
8. Shen H, Smith DE, Yang T, Huang YG, Schnerman JB, Brosius FC. Localization of PEPT1 and PEPT2 proton coupled oligopeptide transporter mRNA and protein in rat kidney. Am J Physiol. 1999;276:F658-F665.
9. Ganapathy V, Leibach FH. Transport Across Intestinal Epithelium In: Yamada T, ed. Textbook of Gastroenterology. Philadelphia, PA: Lippincott Williams and Wilkins; 1999:456-467.
10. Terada T, Sawada K, Saito H, Hashimoto Y, Inui KI. Functional characteristics of basolateral peptide transporter in the human intestinal cell line Caco-2. Am J Physiol. 1999;276:G1435-G1441.
11. Gonzales DE, Covitz KMY, Sadee W, Mrsny R. An oligopeptide transporter is expressed at high levels in the pancreatic carcinoma cell lines AsPc-1 and Capan-2. Cancer Res. 1998; 519-525.
12. Saito H, Motohashi H, Mukai M, Inui K. Cloning and characterization of a pH-sensing regulatory factor that modulates transport activity of the human H+/peptide cotransporter, PEPT1. Biochem Biophys Res Commun. 1997;237:577-582.
13. Fei YJ, Sugawara M, Liu JC, et al. cDNA structure, genomic organization, and promoter analysis of the mouse intestinal peptide transporter PEPT1. Biochem Biophys Acta. 2000;1492:145-154.
14. Shapiro MB, Senapathy P. RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression. Nucl Acids Res. 1987;15:7155-7174.
15. Graul RC, Sadee W. Sequence alignments of the H+-dependent oligopeptide transporter family PTR: inferences on structure and function of the intestinal PEPT1 transporter. Pharm Res. 1997;14:388-400.
16. Fei YJ, Liu JC, Fujita T, Liang R, Ganapathy V, Leibach FH. Identification of a potential substrate binding domain in the mammalian peptide transporters PEPT1 and PEPT2 using PEPT1-PEPT2 and PEPT2-PEPT1 chimeras. Biochem Biophys Res Commun. 1998;246:39-44.
17. Pave-Preux M, Aggerbeck M, Veyssier C, Bousquet-Lamercier B, Hanoune J, Barouki R. Hormonal discrimination among transcription start sites of aspartate amino transferase. J Biol Chem. 1990;265:4444-4448.
18. Walker D, Thwaites DT, Simmons NL, Gilbert HJ, Hirst BH. Substrate upregulation of the human small intestinal peptide transporter, hPept1. J Physiol. 1998;507:697-706.
19. Thamotharan M, Bawani SZ, Zhou X, Adibi SA. Hormonal regulation of oligopeptide transporter PepT1 in a human intestinal cell line. Am J Physiol. 1999;276:C821-C826.