Localization of PEPT1 and PEPT2 proton-coupled oligopeptide transporter mRNA and protein in rat kidney

American Journal of Physiology - Renal Physiology

Volumn 276, Issue 5 45-5, 1999, Pages

  Shen, Hong ^a   Smith, David E ^a,d   Yang, Tianxin ^b   Huang, Yuning G ^b   Schneemann, Jürgen B ^b   Brosius III, Frank C ^b,c  

^a UNIVERSITY OF MICHIGAN   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^c VA ANN ARBOR HEALTHCARE SYSTEM   (United States)

^d PFIZER INC   (United States)

Author keywords

Immunoblots; Immunocytochemistry; Northern blot analysis; Proximal tubules

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA; OLIGOPEPTIDE; POLYCLONAL ANTIBODY; SYNTHETIC PEPTIDE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; GENE EXPRESSION; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; INTESTINE BRUSH BORDER; KIDNEY PROXIMAL TUBULE; KIDNEY TUBULE; MALE; MEMBRANE VESICLE; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTON TRANSPORT; RAT;

EID: 0032967296     PISSN: 1931857X     EISSN: 15221466     Source Type: Journal    
DOI: 10.1152/ajprenal.1999.276.5.f658     Document Type: Article

References (24)

1. Adibi, S. A. The oligopeptide transporter (Pept-1) in human intestine: biology and function. Gastroenterology 113: 332-340, 1997.
2. Adibi, S. A. Renal assimilation of oligopeptides: physiological mechanisms and metabolic importance. Am. J. Physiol. 272 (Endocrinol. Metab. 35): E723-E736, 1997.
3. Akarawut, W., C.-J. Lin, and D. E. Smith. Noncompetitive inhibition of glycylsarcosine transport by quinapril in rabbit renal brush border membrane vesicles: effect on high-affinity peptide transporter. J. Pharmacol. Exp. Ther. 287: 684-690, 1998.
4. Boll, M., D. Markovich, W.-M. Weber, H. Korte, H. Daniel, and H. Murer. Expression cloning of a cDNA from rabbit small intestine related to proton-coupled transport of peptides, β-lactam antibiotics and ACE-inhibitors. Pflügers Arch. 429: 146-149, 1994.
5. Boll, M., M. Herget, M. Wagener, W. M. Weber, D. Markovich, J. Biber, W. Clause, H. Murer, and H. Daniel. Expression cloning and functional characterization of the kidney cortex high-affinity proton-coupled peptide transporter. Proc. Natl. Acad. Sci. USA 93: 284-289, 1996.
6. +/peptide cotransporter. FASEB J. 9: 1489-1496, 1995.
7. Brosius, F. C., III, Y. Liu, N. Nguyen, D. Sun, J. Bartlett, and M. Schwaiger. Persistent myocardial ischemia increases GLUT1 glucose transporter expression in both ischemic and non-ischemic heart regions. J. Mol. Cell. Cardiol. 29: 1675-1685, 1997.
8. Chomczynski, P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15: 532-534, 536-537, 1993.
9. Chomczynski, P., and N. Sacchi. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159, 1987.
10. + symporters. J. Membr. Biol. 154: 197-203, 1996.
11. Fei, Y.-J., Y. Kanai, S. Nussberger, V. Ganapathy, F. H. Leibach, M. F. Romero, S. K. Singh, W. F. Boron, and M. A. Hediger. Expression cloning of a mammalian proton-coupled oligopeptide transporter. Nature 368: 563-566, 1994.
12. Ganapathy, V., and F. H. Leibach. Peptide transporters. Curr. Opin. Nephrol. Hypertens. 5: 395-400, 1996.
13. Hakes, D. J., and J. E. Dixon. New vectors for high level expression of recombinant proteins in bacteria. Anal. Biochem. 202: 293-298, 1992.
14. Leibach, F. H., and V. Ganapathy. Peptide transporters in the intestine and the kidney. Annu. Rev. Nutr. 16: 99-119, 1996.
15. +/peptide cotransporter: cloning, functional expression, and chromosomal localization. J. Biol. Chem. 270: 6456-6463, 1995.
16. +/peptide cotransporter family, from human kidney. Biochim. Biophys. Acta 1235: 461-466, 1995.
17. Marcus, R. G., R. England, K. Nguyen, M. J. Charron, J. P. Briggs, and F. C. Brosius III. Altered renal expression of the insulin-responsive glucose transporter GLUT4 in experimental diabetes mellitus. Am. J. Physiol. 267 (Renal Fluid Electrolyte Physiol. 36): F816-F824, 1994.
18. Miyamoto, K.-I., T. Shiraga, K. Morita, H. Yamamoto, H. Haga, Y. Taketani, I. Tamai, Y. Sai, A. Tsuji, and E. Takeda. Sequence, tissue distribution and developmental changes in rat intestinal oligopeptide transporter. Biochim. Biophys. Acta 1305: 34-38, 1996.
19. +/peptide cotransporter in rat digestive tract. Biochem. Biophys. Res. Commun. 220: 848-852, 1996.
20. +/peptide cotransporter mediating absorption of β-lactam antibiotics in the intestine and kidney. J. Pharmacol. Exp. Ther. 275: 1631-1637, 1995.
21. Saito, H., T. Terada, M. Okuda, S. Sasaki, and K.-I. Inui. Molecular cloning and tissue distribution of rat peptide transporter PEPT2. Biochim. Biophys. Acta 1280: 173-177, 1996.
22. + gradient-driven dipeptide reabsorption in proximal tubule of rat kidney: studies in vivo and in vitro. Am. J. Physiol. 253 (Renal Fluid Electrolyte Physiol. 22): F448-F457, 1987.
23. Smith, D. E., A. Pavlova, U. V. Berger, M. A. Hediger, T. Yang, Y. G. Huang, and J. B. Schnermann. Tubular localization and tissue distribution of peptide transporters in rat kidney. Pharm. Res. 15: 1244-1249, 1998.
24. Tanaka, H., K.-I. Miyamoto, K. Morita, H. Haga, H. Segawa, T. Shiraga, A. Fujioka, T. Kouda, Y. Taketani, S. Hisano, Y. Fukui, K. Kitagawa, and E. Takeda. Regulation of the PepT1 peptide transporter in the rat small intestine in response to 5-fluorouracil-induced injury. Gastroenterology 114: 714-723, 1998.