The potential of a polyphasic PCR-DGGE approach in evaluating microbial diversity of natural whey cultures for water-buffalo Mozzarella cheese production: Bias of culture-dependent and culture-independent analyses

Systematic and Applied Microbiology

Volumn 24, Issue 4, 2001, Pages 610-617

  Ercolini, Danilo ^a   Moschetti, Giancarlo ^a   Blaiotta, Giuseppe ^a   Coppola, Salvatore ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

"culture independent"; "culture dependent"; Natural whey cultures; PCR DGGE analysis

Indexed keywords

AGAR; BACTERIAL DNA; DNA 16S;

ARTICLE; BACTERIAL GENETICS; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BUFFALO; CHEESEMAKING; CONTROLLED STUDY; DAIRY INDUSTRY; DENATURING GRADIENT GEL ELECTROPHORESIS; ENTEROCOCCUS FAECALIS; GENETIC ANALYSIS; LACTIC ACID BACTERIUM; LACTOBACILLUS; LACTOBACILLUS CRISPATUS; LACTOBACILLUS DELBRUECKII; LACTOBACILLUS FERMENTUM; LACTOCOCCUS LACTIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STREPTOCOCCUS THERMOPHILUS; WHEY;

BACTERIA (MICROORGANISMS); BUBALUS; BUBALUS BUBALIS; ENTEROCOCCUS; ENTEROCOCCUS FAECALIS; LACTOBACILLUS; LACTOBACILLUS CRISPATUS; LACTOBACILLUS DELBRUECKII; LACTOBACILLUS FERMENTUM; LACTOCOCCUS; LACTOCOCCUS LACTIS; POSIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS THERMOPHILUS;

EID: 0035708608     PISSN: 07232020     EISSN: None     Source Type: Journal    
DOI: 10.1078/0723-2020-00076     Document Type: Article

References (38)

1. Molecular ecology of microbes: A review of promises, pitfalls and true progress
2. Phylogenetic identification and in situ detection of individual microbial cells without cultivation
3. Cluster analysis, richness and biodiversity indexes derived from denaturing gradient gel electrophoresis fingerprints of bacterial communities demonstrate that traditional maize fermentations are driven by the transformation process
4. Polyphasic study of the spatial distribution of microorganisms in mexican pozol, a fermented maize dough, demonstrates the need for cultivation-independent methods to investigate traditional fermentations
5. Culture-independent quantification of physiologically active microbial groups in fermented foods using rRNA-targeted oligonucleotide probes: Application to pozol, a Mexican lactic acid fermented maize dough
6. The microflora of natural whey cultures utilized as starters in the manufacture of Mozzarella cheese from water-buffalo milk
7. Comparison of different starter systsems for water-buffalo Mozzarella cheese manufacture
8. Monitoring impact of a pesticide treatment on bacterial soil communities by metabolic and genetic fingerprinting in addition to conventional testing procedures
9. Behavior of variable V 3 region from 16S rDNA of lactic acid bacteria in denaturing gradient gel electrophoresis
10. Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis
11. PCR-based detection of verotoxin-producing Escherichia coli (VTEC) in ground beef
12. Diversity and structure of the methanogenic community in anoxic rice paddy soil microcosms as examined by cultivation and direct 16S rRNA gene sequence retrieval
13. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoresis in denaturing gradients
14. Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity
15. Changes in bacterial species composition in enrichment cultures with various dilutions of inoculum as monitored by denaturing gradient gel electrophoresis
16. Microbial diversity in soil: The need for a combined approach using molecular and cultivation techniques
17. Proteolytic activity of lactococcal strains from water-buffalo Mozzarella starter cultures
18. A selected medium for detecting leuconostoc organisms in mixed strains starter cultures
19. Nisinproducing organisms during traditional "Fior di latte" cheese-making monitored by Multiplex-PCR and PFGE analyses
20. Random amplified polymorphic DNA (RAPD) and amplified ribosomal DNA spacer polymorphism: Powerful methods to differentiate Streptococcus thermophilus strains
21. Presence of non-functional nisin genes in Lactococcus lactis subsp. Lactis isolated from natural starters
22. DGGE/TGGE a method for identifying genes from natural ecosystems
23. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology
24. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA
25. Microbial community dynamics during production of the mexican fermented maize dough pozol
26. DNA probe and PCR-specific reaction for Lactobacillus plantarum
27. Monitoring complex bacterial communities using culture-independent molecular techniques: Application to soil environment
28. Differential amplification of rRNA genes by polymerase chain reaction
29. DNA sequencing with chain-terminating inhibitors
30. Denaturing gradient gel electrophoresis used to monitor the enrichment culture of aerobic chemoorganotrophic bacteria from hot spring cyanobacterial mat
31. Application of denaturing gradient gel electrophoresis for the analysis of the porcine gastrointestinal microbiota
32. Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR
33. Specific ribosomal DNA sequences from diverse environmental settings correlate with experimental contaminants
34. Molecular identification of bacteria from coculture by denaturing gradient gel electrophoresis of 16S ribosomal DNA fragments as a tool of isolation in pure culture
35. Evaluation of denaturing gradient gel electrophoresis in the detection of 16S rDNA sequence variation in rhizobia and methanotrophs
36. Characterization of strains of Leuconostoc mesenteroides by analysis of soluble whole-cell protein pattern, DNA fingerprinting and restriction of ribosomal DNA
37. 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community
38. Determination of microbial diversity in environmental samples: Pitfalls of PCR-based rRNA analysis