Presence of non-functional nisin genes in Lactococcus lactis subsp. lactis isolated from natural starters

FEMS Microbiology Letters

Volumn 145, Issue 1, 1996, Pages 27-32

  Moschetti, Giancarlo ^a   Villani, Francesco ^a   Blaiotta, Giuseppe ^a   Baldinelli, Antonio ^a   Coppola, Salvatore ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Lactococcus lactis subsp. lactis; Natural whey starter; Nisin structural gene; Pulsed field gel electrophoresis; Reverse transcriptase polymerase chain reaction

Indexed keywords

BACTERIOCIN; DNA; NISIN;

ARTICLE; CONTROLLED STUDY; DNA HYBRIDIZATION; GENE; LACTOCOCCUS LACTIS; NONHUMAN; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING;

LACTOCOCCUS; LACTOCOCCUS LACTIS; LACTOCOCCUS LACTIS SUBSP. LACTIS; STREPTOCOCCUS;

EID: 0030589180     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1097(96)00380-1     Document Type: Article

References (22)

1. [1] Schnell, N., Entian, K.D., Schneider, U., Gotz, F., Zahner, H., Kellner, R. and Jung, G. (1988) Peptide sequence of epidermin, a ribosomally synthesized antibiotic with four sulphide-rings. Nature 333, 276-278.
2. [2] Molders, J.W.M., Boerrigter, I.J., Rollema, H.S., Siezen, R.J. and De Vos, W.M. (1991) Identification and characterization of the lantibiotic nisin Z, a natural nisin variant. Eur. J. Biochem. 201, 581-584.
3. [3] Hansen, J.N. (1990) Structure, expression and evolution of the nisin gene locus in Lactococcus lactis. Program Abstr. 3rd Int. ASM Conference in streptococcal genetics. ASM, Washington, DC, abstr. 15, 12.
4. [4] Steen, M.T., Chung, Y.J. and Hansen, J.N. (1991) Characterization of the nisin gene as part of a polycistronic operon in the chromosome of Lactococcus lactis ATCC 11454. Appl. Environ. Microbiol. 57, 1181-1188.
5. [5] Gireesh, T., Davidson, B.E. and Hillier, A.J. (1992) Conjugal transfer in Lactococcus lactis of a 68-kilobase-pair chromosomal fragment containing the structural gene for the peptide bacteriocin nisin. Appl. Environ. Microbiol. 58, 1670-1676.
6. [6] Rauch, P.J.G. and De Vos, W.M. (1992) Characterization of the novel nisin-sucrose conjugative transposon Tn5276 and its insertion in Lactococcus lactis. J. Bacteriol. 174, 1280-1287.
7. [7] Rodriguez, J.M., Cintas, L.M., Casaus P., Horn, N., Dodd, H.M., Hernandez, P.E. and Gasson, M.J. (1995) Isolation of nisin-producing Lactococcus lactis strains from dry fermented sausages. J. Appl. Bacteriol. 78, 109-115.
8. [8] Klijn, N., Weerkamp, H.A. and De Vos, W.M. (1995) Detection and characterization of lactose-utilizing Laclacoccus spp. in natural ecosystems. Appl. Environ. Microbiol. 61, 788-792.
9. [9] Wang, A.M., Doyle, M.V. and Mark, D.F. (1989) Quantitation of mRNA by the polymerase chain reaction. Proc. Natl. Acad. Sci. USA 86, 9717-9721.
10. [10] Rutiar Ra, S. and Saris, P.E.J. (1995) Characterization of prokaryotic mRNA by RT-PCR. Biotechnology 18, 792-795.
11. [11] Villani, F., Pepe, O., Moschetti, G., Salzano, G., Parente, E. and Coppola, S. (1995) Antagonistic activity of lactic acid bacteria isolated from natural whey starters for water-buffalo Mozzarella cheese manufacture, Ital. J. Food Sci. 3, 221-234.
12. [12] Coppola, S., Parente, E., Dumontet, S. and La Peccerella, A. (1988) The microflora of natural whey cultures utilized as starters in the manufacture of Mozzarella cheese from water-buffalo milk. Lait 68, 295-310.
13. [13] Ward, L.J.H., Brown, J.C.S. and Davey, G.P. (1994) Application of the ligase chain reaction to the detection of nisin A and nisin Z genes in Lactococcus lactis ssp. lactis. FEMS Microbiol. Lett. 117, 29-34.
14. [14] McClelland, M., Jones, R., Patel, Y. and Nelson, M. (1987) Restriction endonuclease for pulsed field mapping of bacterial genomes. Nucleic Acids Res. 15, 5985-6005.
15. [15] Chevallier, B., Hubert, J.-C. and Kammerer, B. (1994) Determination of chromosome size and number of rrn loci in Lactobacillus plantarum by pulsed-field gel electrophoresis. FEMS Microbiol. Lett. 120, 51-56.
16. [16] Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, COld Spring Harbor, NY.
17. [17] Southern, E. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503-517.
18. [18] Oelmuller, U., Kruger, N., Steinbuchel, A. and Friedrich, C.G. (1990) Isolation of prokaryotic RNA and detection of specific mRNA with biotinylated probes. J. Microbiol. Methods 11, 73-84.
19. [19] Neve, H., Geis, A. and Teuber, M. (1987) Conjugation, a common plasmid transfer mechanism in lactic acid streptococci of dairy starter cultures. Syst. Appl. Microbiol., 9 151-157.
20. [20] Gabin-Gauthier, K., Gratadoux, J.-J. and Richard, J. (1991) Conjugal plasmid transfer between lactococci on solid surface matings and during cheese making. FEMS Microbiol. Lett. 85, 133-140.
21. [21] Rauch, P.J.G., Beerthuyzen, M.M. and De Vos, W.M. (1991) Molecular analysis and evolution of conjugative transposons encoding nisin production and sucrose fermentation in Lactococcus lactis. In: Nisin and Novel Lantibiotics (Jung, G. and Shal, H.G., Eds.), ESCOM, Leiden, pp. 243-249.
22. [22] Rauch, P.J.G., Kuipers, O.P., Siezen, R.J and De Vos, W.M. (1994) Genetics and protein engineering of nisin. In: Bacteriocins of Lactic Acid Bacteria (De Vuyst, L. and Vandamme, E.I., Eds.), Blackie A and P, London, pp. 223-249.