Quantification of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) mRNA in testicular tissue of infertile patients

Asian Journal of Andrology

Volumn 3, Issue 4, 2001, Pages 263-270

  Schrader, M ^a   Muller M ^a   Heicappell, R ^a   Straub, B ^a   Miller, K ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Fertility; Human telomerase reverse transcriptase; Human telomerase RNA; Spermatogenesis

Indexed keywords

MESSENGER RNA; RNA; RNA DIRECTED DNA POLYMERASE; TELOMERASE;

ARTICLE; AZOOSPERMIA; CELL MATURATION; CLINICAL ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CRYOPRESERVATION; DIAGNOSTIC VALUE; DISCRIMINANT ANALYSIS; FLUORESCENCE ANALYSIS; GAMETOGENESIS; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; MALE; MALE INFERTILITY; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SERTOLI CELL; SPERMATOGENESIS; TESTIS BIOPSY; TISSUE PREPARATION;

DNA-BINDING PROTEINS; GENE EXPRESSION; HUMANS; MALE; OLIGOSPERMIA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, MESSENGER; SERTOLI CELLS; SPERM MATURATION; SPERMATOGENESIS; TELOMERASE; TESTIS;

EID: 0035670157     PISSN: 1008682X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (43)

1. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte
2. Criteria predicting the absence of spermatozoa in the sertoli cell-only syndrome can be used to improve success rates of sperm retrieval
3. Distribution of spermatogenesis in the testicles of azoospermic men: The presence or absence of spermatids in the testes of men with germinal failure
4. Are there any predictive factors for successful testicular sperm recovery in azoospermic patients?
5. Testicular sperm extraction: Comprehensive analysis with simultaneously performed histology in 1418 biopsies from 766 subfertile men
6. The telomere terminal transferase of Tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity
7. Structure and function of telomeres
8. Telomeres shorten during ageing of human fibroblasts
9. Specific association of human telomerase activity with immortal cells and cancer
10. The RNA component of human telomerase
11. Telomerase catalytic subunit homologs from fission yeast and human
12. Human telomerase contains evolutionarily conserved catalytic and structural subunits
13. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization
14. Isolation of a candidate human telomerase catalytic subunit gene, which reveals complex splicing patterns in different cell types
15. A mammalian telomerase-associated protein
16. Tankyrase, a poly (ADP-ribose) polymerase at human telomeres
17. Telomerase activity in the testis of infertile patients with selected causes
18. Telomerase activity and expression of telomerase subunits in the testicular tissue of infertile patients
19. Highly sensitive quantitative telomerase assay of diagnostic testicular biopsy material predicts the presence of haploid spermatogenic cells in therapeutic testicular biopsy in men with sertoli cell-only syndrome
20. Cryopreservation of testicular tissue: A highly effective method to provide sperm for successful TESE/ICSI procedures
21. Testicular biopsy score count - A method for registration of spermatogenesis in human testes: Normal values and results in 335 hypogonadal males
22. Histopathology of human testicular and epididymal tissue
23. Telomerase activity and expression of telomerase subunits in the testicular tissue of infertile patients
24. Continuous fluorescence monitoring of rapid cycle DNA amplification
25. Molecular diagnostic detection of free cancer cells in the peritoneal cavity of patients with gastrointestinal and gynecologic malignancies
26. LightCycler technology for the quantitation of bcr/abl fusion transcripts
27. Accurate and rapid analysis of residual disease in patients with CML using specific fluorescent hybridization probes for real time quantitative RT-PCR
28. Rapid quantitative detection of carcinoembryonic antigen-expressing free tumor cells in the peritoneal cavity of gastric-cancer patients with real-time RT-PCR on the lightcycler
29. Rapid quantitative detection of BCR-ABL transcripts in chronic myeloid leukemia patients by real-time reverse transcriptase polymerase-chain reaction using fluorescently labeled probes
30. Retinal VEGF mRNA measured by SYBR green I fluorescence: A versatile approach to quantitative PCR
31. Different real-time PCR formats compared for the quantitative detection of human cytomegalovirus DNA
32. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
33. The LightCycler: A microvolume multisample fluorimeter with rapid temperature control
34. A novel highly reproducible quantitative competitve RT PCR system
35. Two variants of quantitative reverse transcriptase PCR used to show differential expression of alpha-, beta- and gamma-fibrinogen genes in rat liver lobes
36. Developmental and tissue-specific regulation of mouse telomerase and telomere length
37. Differentiation of immortal cells inhibits telomerase activity
38. Telomerase activity in female and male rat germ cells undergoing meiosis and in early embryos
39. Loss of telomerase activity during male germ cell differentiation
40. Postmeiotic modifications of spermatogenic cells are accompanied by inhibition of telomerase activity
41. Telomerase activity in testicular biopsy material
42. Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues
43. Reconstitution of human telomerase activity and identification of a minimal functional region of the human telomerase RNA