Structural and functional characterization of the 5′ upstream promoter of the human Phox2a gene: Possible direct transactivation by transcription factor Phox2b

Journal of Neurochemistry

Volumn 79, Issue 6, 2001, Pages 1225-1236

  Hong, Seok Jong ^a   Kim, Chun Hyung ^a   Kim, Kwang Soo ^a  

^a MCLEAN HOSPITAL   (United States)

Author keywords

Cell type specific transcription; Norepinephrine; Phox2a; Phox2b; Promoter; Transcription start site

Indexed keywords

COMPLEMENTARY DNA; LUCIFERASE; MESSENGER RNA; NORADRENALIN; NUCLEOTIDE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR PHOX2A; TRANSCRIPTION FACTOR PHOX2B; UNCLASSIFIED DRUG;

ARTICLE; EXON; GENE EXPRESSION; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INTRON; NERVE CELL DIFFERENTIATION; NORADRENERGIC NERVE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; START CODON; TATA BOX; TRANSACTIVATION; TRANSCRIPTION INITIATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS; CLONING, MOLECULAR; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, NEOPLASTIC; GENES; GENES, REPORTER; HELA CELLS; HOMEODOMAIN PROTEINS; HUMANS; LUCIFERASES; MOLECULAR SEQUENCE DATA; NEOPLASM PROTEINS; NERVE TISSUE PROTEINS; NEUROBLASTOMA; NEURONS; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; RNA, NEOPLASM; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0035661055     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.2001.00672.x     Document Type: Article

References (42)

1. Paired-like homeodomain proteins Phox2a/Arix and Phox2b/NBPhox have similar genetic organization and independently regulate dopamine betahydroxylase gene transcription
2. Cyclic AMP signaling functions as a bimodal switch in sympathoadrenal cell development in cultured primary neural crest cells
3. Sp proteins and Phox2b regulate the expression of the human Phox2a gene
4. Transcriptional control of neurotransmitter phenotype
5. Development of noradrenergic neurons in the zebrafish hindbrain requires BMP, FGF8, and the homeodomain protein soulless/Phox2a
6. Databases on transcriptional regulation: TRANSFAC, TRRD and COMPEL
7. Control of noradrenergic differentiation and Phox2a expression by MASH1 in the central and peripheral nervous system
8. Determination of messenger RNA 5′-ends by reverse transcription of the cap structure
9. Cell-specific expression from the human dopamine beta-hydroxylase promoter in transgenic mice is controlled via a combination of positive and negative regulatory elements
10. Neuron-specific expression of the human dopamine beta-hydroxylase gene requires both the cAMP-response element and a silencer region
11. A PCR-based method for high stringency screening of DNA libraries
12. A previously undescribed intron and extensive 5′ upstream sequence, but not Phox2a-mediated transactivation, are necessary for high level cell type-specific expression of the human norepinephrine transporter gene
13. Noradrenergic-specific transcription of the dopamine betahydroxylase gene requires synergy of multiple cis-acting elements including at least two Phox2a-binding sites
14. Both the basal and inducible transcription of the tyrosine hydroxylase gene are dependent upon a cAMP response element
15. The cAMP-dependent protein kinase regulates transcription of the dopamine beta-hydroxylase gene
16. Analysis of gene expression by blotting
17. Genetic alteration of catecholamine specificity in transgenic mice
18. Functional and high level expression of human dopamine beta-hydroxylase in transgenic mice
19. MASH1 activates expression of the paired homeodomain transcription factor Phox2a, and couples pan-neuronal and subtype-specific components of autonomic neuronal identity
20. Specification of neurotransmitter identity by Phox2 proteins in neural crest stem cells
21. The dopamine beta-hydroxylase gene promoter directs expression of E. coli lacZ to sympathetic and other neurons in adult transgenic mice
22. Defects in sensory and autonomic ganglia and absence of locus coeruleus in mice deficient for the homeobox gene Phox2a
23. The 5′-flanking region of the human dopamine beta-hydroxylase gene promotes neuron subtype-specific gene expression in the central nervous system of transgenic mice
24. Isolation and sequence-specific DNA binding of the Antennapedia homeodomain
25. Expression and interactions of the two closely related homeobox genes Phox2a and Phox2b during neurogenesis
26. The homeobox gene Phox2b is essential for the development of autonomic neural crest derivatives
27. Control of hindbrain motor neuron differentiation by the homeobox gene Phox2b
28. Involvement of bone morphogenetic protein-4 and bone morphogenetic: Protein-7 in the differentiation of the adrenergic phenotype in developing sympathetic neurons
29. Neurotransmitter-synthesizing enzymes in 14 human neuroblastoma cell lines
30. Bone morphogenetic proteins are required in vivo for the generation of sympathetic neurons
31. Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines
32. Multiple protein factors interact with the cis-regulatory elements of the proximal promoter in a cell-specific manner and regulate transcription of the dopamine beta-hydroxylase gene
33. The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons
34. The homeodomain protein Arix interacts synergistically with cyclic AMP to regulate expression of neurotransmitter biosynthetic genes
35. AP1 proteins mediate the cAMP response of the dopamine beta-hydroxylase gene
36. The homeodomain protein Arix promotes protein kinase A-dependent activation of the dopamine beta-hydroxylase promoter through multiple elements and interaction with the coactivator cAMP-response element-binding protein-binding protein
37. The expression pattern of the transcription factor Phox2 delineates synaptic pathways of the autonomic nervous system
38. The mouse homeodomain protein Phox2 regulates Ncam promoter activity in concert with Cux/CDP and is a putative determinant of neurotransmitter phenotype
39. Expression of a constitutively active type I BMP receptor using a retroviral vector promotes the development of adrenergic cells in neural crest cultures
40. Identification and characterization of potential cis-regulatory elements governing transcriptional activation of the rat tyrosine hydroxylase gene
41. Paired-like homeodomain proteins, Phox2a and Phox2b, are responsible for noradrenergic cell-specific transcription of the dopamine beta-hydroxylase gene
42. A homeodomain protein selectively expressed in noradrenergic tissue regulates transcription of neurotransmitter biosynthetic genes