Development and improvement of a serum-free suspension process for the production of recombinant adenoviral vectors using HEK293 cells

Cytotechnology

Volumn 37, Issue 3, 2001, Pages 189-198

  Tsao, Yung Shyeng ^a   Condon, Russell ^a   Schaefer, Eugene ^a   Lio, Peggy ^a   Liu, Zhong ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Adenovirus; Dimethyl sulfoxide (DMSO); Ethyl alcohol; Gene therapy; Human Embryonic Kidney 293 (HEK293) cells; N acetyl L cysteine; Recombinant adenoviral vector; Serum free suspension culture; Sodium butyrate

Indexed keywords

ADENOVIRIDAE;

EID: 0035550428     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1020555310558     Document Type: Article

References (45)

1. Amalfitano A. 1999. Next-generation adenoviral vectors: New and improved. Gene. Ther. 6: 1643-1645.
2. Anderson W.F. 1998. Human gene therapy. Nature 392: 25-30.
3. Arts J., Lansink M., Grimbergen J., Toet K.H. and Kooistra T. 1995. Stimulation of tissue-type plasminogen activator gene expression by sodium butyrate and trichostatin A in human endothelial cells involves histone acetylation. Biochem. J. 310: 171-176.
4. Bazzoni G., Beltran Nunez A., Mascellani G., Bianchini P., Dejana E. and Del Maschio A. 1993. Effect of heparin, dermatan sulfate, and related oligo-derivatives on human polymorphonuclear leukocyte functions. J. Lab. Clin. Med. 121: 268-275.
5. Benihoud K., Yeh P. and Perricaudet M. 1999. Adenovirus vectors for gene delivery. Curr. Opin. Biotechnol. 10: 440-447.
6. Berg D.T., McClure D.B. and Grinnell B.W. 1993. High-level expression of secreted proteins from cells adapted to serum-free suspension culture. Biotechniques 14: 972-978.
7. Collins S.J., Ruscetti F.W., Gallagher R.E. and Gallo R.C. 1978. Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc. Natl. Acad. Sci. USA 75: 2458-2462.
8. Cote J., Garnier A., Massie B. and Kamen A. 1998. Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells. Biotechnol. Bioeng. 59: 567-575.
9. Dee K.U., Shuler M.L. and Wood H.A. 1997a. Inducing single-cell suspension of BTI-TN5B1-4 insect cells: I. The use of sulfated polyanions to prevent cell aggregation and enhance recombinant protein production. Biotechnol. Bioeng. 54: 191-205.
10. Dee K.U., Wood H.A. and Shuler M.L. 1997b. Inducing single-cell suspension of BTI-TN5B1-4 insect cells: II. The effect of sulfated polyanions on baculovirus infection. Biotechnol. Bioeng. 54: 206-220.
11. Eldor A. and Weksler B.B. 1979. Heparin and dextran sulfate antagonize PGI2 inhibition of platelet aggregation. Thromb. Res. 16: 617-628.
12. Fiore M. and Degrassi F. 1999. Dimethyl sulfoxide restores contact inhibition-induced growth arrest and inhibits cell density-dependent apoptosis in hamster cells. Exp. Cell Res. 251: 102-110.
13. Frank W.O., Rayyes A.N., Washington A. and Holt P.R. 1979. Effect of acute ethanol administration upon hepatic regeneration. J. Lab. Clin. Med. 93: 402-413.
14. Garnier A., Cote J., Nadeau I., Kamen A. and Massie B. 1994. Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells. Cytotechnology 15: 145-155.
15. Gorman C.M., Howard B.H. and Reeves R. 1983. Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate. Nucleic Acids Res. 11: 7631-7648.
16. Graham F.L. 2000. Adenovirus vectors for high-efficiency gene transfer into mammalian cells. Immunol. Today 21: 426-428.
17. Graham F.L., Smiley J., Russell W.C. and Nairn R. 1977. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36: 59-74.
18. Greber U.F., Willetts M., Webster P. and Helenius A. 1993. Stepwise dismantling of adenovirus 2 during entry into cells. Cell 75: 477-486.
19. Hu W.S. and Aunins J.G. 1997. Large-scale mammalian cell culture. Curr. Opin. Biotechnol. 8: 148-153.
20. Irvine Scientific, Inc. 1997. Product Description and Applications (PN 99269 Rev2 1/97) IS 293 (Catalog No. 99269).
21. Iyer P., Ostrove J.M. and Vacante D. 1999. Comparison of manufacturing techniques for adenovirus production. Cytotechnology 30: 169-172.
22. Kallos M.S., Behie L.A. and Vescovi A.L. 1999. Extended serial passaging of mammalian neural stem cells in suspension bioreactors. Biotechnol. Bioeng. 65: 589-599.
23. Kameneva M.V., Antaki J.F., Watach M.J., Borovetz H.S. and Kormos R.L. 1994. Heparin effect on red blood cell aggregation. Biorheology 31: 297-304.
24. Katayose D. and Seth P. 1999. Chapter 10: Development of adenoviral vectors for gene therapy. In: Seth P. (ed.), Adenoviruses: Basic Biology to Gene Therapy. R.G. Landers Company, Austin, TX. pp. 91-101.
25. Malorni W., Rivabene R., Santini M.T. and Donelli G. 1993. N-acetylcysteine inhibits apoptosis and decreases viral particles in HIV-chronically infected U937 cells. FEBS Lett. 327: 75-78.
26. Mastrangelo A.J., Zou S., Hardwick J.M. and Betenbaugh M.J. 1999. Antiapoptosis chemicals prolong productive lifetimes of mammalian cells upon Sindbis virus vector infection. Biotechnol. Bioeng. 65: 298-305.
27. Mather J.P., Moore A. and Shawley R. 1997. Optimization of growth, viability, and specific productivity for expression of recombinant proteins in mammalian cells. Methods Mol. Biol. 62: 369-382.
28. Nadeau I., Garnier A., Cote J., Massie B., Chavarie C. and Kamen A. 1996. Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies. Biotechnol. Bioeng. 51: 613-623.
29. Nakano M.Y. and Greber U.F. 2000. Quantitative microscopy of fluorescent adenovirus entry. J. Struct. Biol. 129: 57-68.
30. Peshwa M.V., Kyung Y.S., McClure D.B. and Hu W.S. 1993. Cultivation of mammalian cells as aggregates in bioreactors: Effect of calcium concentration on spatial distribution of viability. Biotechnol. Bioeng. 41: 179-187.
31. Reches A., Eldor A. and Salomon Y. 1979. The effects of dextran sulfate, heparin and PGEI on adenylate cyclase activity and aggregation of human platelets. Thromb. Res. 16: 107-116.
32. Robbins P.D. and Ghivizzani S.C. 1998. Viral vectors for gene therapy. Pharmacol. Ther. 80: 35-47.
33. Rochon Y.P., Simon S.I., Lynam E.B. and Sklar L.A. 1994. A role for lectin interactions during human neutrophil aggregation. J. Immunol. 152: 1385-1393.
34. Russell W.C. 2000. Update on adenovirus and its vectors. J. Gen. Virol. 81(Pt 11): 2573-2604.
35. Saito H., Kagawa T., Tada S., Tsunematsu S., Guevara F.M., Watanabe T. et al. 1992. Effect of dexamethasone, dimethylsulfoxide and sodium butyrate on a human hepatoma cell line PLC/PRF/5. Cancer Biochem. Biophys. 13: 75-84.
36. Schmid S.I. and Hearing P. 1999. Chapter 9: Adenovirus Assembly. In: Seth P. (ed.), Adenoviruses: Basic Biology to Gene Therapy. R.G. Landers Company, Austin. TX, pp. 85-89.
37. Schoofs G., Monica T.J., Ayala J., Horwitz J., Montgomery T., Roth G. et al. 1998. A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy. Cytotechnology 28: 81-89.
38. Shabram P.W., Giroux D.D., Goudreau A.M., Gregory R.J., Horn M.T., Huyghe B.G. et al. 1997. Analytical anion-exchange HPLC of recombinant type-5 adenoviral particles. Hum. Gene. Ther. 8: 453-465.
39. Shenk T. 1995. Chapter 3: Group C adenoviruses as vectors for gene therapy. In: Kaplitt M.G. and Loewy A.D. (eds), Viral Vectors: Gene Therapy and Neuroscience Applications. Academic Press, New York, pp. 43-54.
40. Shenk T. 1996. Chapter 67: Adenoviridae: The viruses and their replication. In: Fields B.N., Knipe D.M. and Howley P.M. (eds), Fields Virology. 3rd Edn. Lippincott - Raven Publishers, Philadelphia, pp. 2111-2148.
41. Spier R.E. 1997. Factors limiting the commercial application of animal cells in culture. Cytotechnology 23: 113-117.
42. Stanley M.J., Liebersbach B.F., Liu W., Anhalt D.J. and Sanderson R.D. 1995. Heparan sulfate-mediated cell aggregation. Syndecans-1 and -4 mediate intercellular adhesion following their transfection into human B lymphoid cells. J. Biol. Chem. 270: 5077-5083.
43. Tsao D., Shi Z.R., Wong A. and Kim Y.S. 1983. Effect of sodium butyrate on carcinoembryonic antigen production by human colonic adenocarcinoma cells in culture. Cancer Res. 43: 1217-1222.
44. Tsao Y.S., Condon R.G., Schaefer E.J., Lindsay D.A. and Liu Z. 2000. Biomass and aggregation analysis of human embryonic kidney 293 suspension cell cultures by particle size measurement. Biotechnol. Prog. 16: 809-814.
45. Wands J.R., Carter E.A., Bucher N.L. and Isselbacher K.J. 1979. Inhibition of hepatic regeneration in rats by acute and chronic ethanol intoxication. Gastroenterology 77: 528-531.