A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy

Cytotechnology

Volumn 28, Issue 1-3, 1998, Pages 81-89

  Schoofs, G ^a   Monica, T J ^a   Ayala, J ^a   Horwitz, J ^a   Montgomery, T ^a   Roth, G ^a   Castillo, F J ^a  

^a BAYER AG   (Germany)

Author keywords

Adenovirus; HEK 293; Perfusion; Serum free media; Suspension perfusion

Indexed keywords

ADENOVIRUS; CELL CULTURE; EXPRESSION VECTOR; GENE THERAPY; RECOMBINANT DNA TECHNOLOGY;

ADENOVIRIDAE;

EID: 0032425329     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1008021428969     Document Type: Article

References (10)

1. Crystal, RG (1995) Transfer of genes to humans: early lessons and obstacles to success. Science 270: 404-410.
2. Garnier A, Cote J, Nadeau I, Kamen A and Massie B (1994) Scaleup of the adenovirus expression system for the production of recombinant protein in human 293S cells. Cytotechnology 15: 145-155.
3. Graham FL and Prevec L (1991) Manipulation of adenovirus vectors. In: Murray EJ (ed.) Methods in Molecular Biology. Vol. 7, The Humana Press, Clifton, NJ, pp. 109-128.
4. Green M and Wold WSM (1979) Human adenoviruses: Growth, purification, and transfection assay. Meth Enzym 58: 425-435.
5. Hehir K, Keegan J, Martin J, Pratt D, Auger C, Davis M, Everton M, Narayana R and Karey K (1998) Production issues for adenoviral gene therapy vectors. Presented at Cell Culture Engineering VI, February 7-12, 1998, San Diego, CA.
6. Marcel T and Grausz JD (1997) The TMC worldwide gene therapy enrollment report, end 1996. Human Gene Therapy 8: 775-800.
7. Nadeau I, Garnier A, Cote J, Massie B, Chavarie C and Kamen A (1996) Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies. Biotech Bioeng 51: 613-623.
8. Nielsen LK, Smyth GK and Greenfield PF (1992) Accuracy of the endpoint assay for virus titration. Cytotechnology 8: 231-236.
9. Roth G, Smith C, Schoofs GM, Montgomery TJ, Ayala JL and Horwitz JI (1997) Using an external vortex flow filtration device for perfusion cell culture. BioPharm 10: 30-35.
10. Shabram PW, Giroux DD, Goudreau AM, Gregory RJ, Horn MT, Huyghe BG, Liu X, Nunnally MH, Sugarman BJ and Sutjipto S (1997) Analytical anion-exchange HPLC of recombinant type-5 adenoviral particles. Human Gene Therapy 8: 453-465.