Inducing single-cell suspension of BTI-TN5B1-4 insect cells: I. The use of sulfated polyanions to prevent cell aggregation and enhance recombinant protein production

Biotechnology and Bioengineering

Volumn 54, Issue 3, 1997, Pages 191-205

  Dee, Kennie U ^a   Shuler, Michael L ^a   Wood, H Alan ^b  

^a Cornell University   (United States)

^b United States Department of Agriculture Agricultural Research Service and Boyce Thompson Institute for Plant Research   (United States)

Author keywords

baculovirus; BTI TN5B1 4; single cell; suspension

Indexed keywords

AGGLOMERATION; CELLS; GENETIC ENGINEERING; PROTEINS; VIRUSES;

BACULOVIRUS EXPRESSION VECTOR SYSTEM; RECOMBINANT PROTEIN; SINGLE CELL SUSPENSION; SULFATED POLYANIONS;

ANIMAL CELL CULTURE;

ALKALINE PHOSPHATASE; BETA GALACTOSIDASE; DEXTRAN SULFATE; DISACCHARIDE; GLYCOSYLATED PROTEIN; MONOSACCHARIDE; POLYANION; POLYCATION; POLYMER; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CELL AGGREGATION; CELL DENSITY; CELL GROWTH; CELL SUSPENSION; CELL VOLUME; CONTROLLED STUDY; ENZYME ACTIVITY; INSECT; MONOLAYER CULTURE; NONHUMAN; PROTEIN SYNTHESIS; SULFATION; SURFACE CHARGE; VIRUS ADSORPTION;

EID: 0031554259     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19970505)54:3<191::AID-BIT1>3.0.CO;2-A     Document Type: Article

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