Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies

Biotechnology and Bioengineering

Volumn 51, Issue 6, 1996, Pages 613-623

  Nadeau, I ^a,b   Garnier, A ^a   Cote J ^a   Massie, B ^a   Chavarie, C ^b   Kamen, A ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

Author keywords

293S cells; adenovirus; fed batch; glucose control; lactate; osmolarity; protein production

Indexed keywords

ADDITION REACTIONS; AMINO ACIDS; BATCH CELL CULTURE; CELLS; CHEMICAL VARIABLES CONTROL; GLUCOSE; PH; VIRUSES;

ADENOVIRUS; FED BATCH PROCESS; INCREASED OSMOLARITY; LACTIC ACID ACCUMULATION; RECOMBINANT PROTEIN PRODUCTION; TYROSINE PHOSPHATASE;

PROTEINS;

BICARBONATE; ESSENTIAL AMINO ACID; GLUCOSE; LACTIC ACID; PROTEIN TYROSINE PHOSPHATASE; RECOMBINANT PROTEIN; SERINE; VIRUS VECTOR;

ARTICLE; BIOREACTOR; CELL CULTURE; CELL DENSITY; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; HUMAN; HUMAN ADENOVIRUS; HUMAN CELL; OSMOLARITY; PH;

ADENOVIRIDAE; HUMAN ADENOVIRUS;

EID: 9444244378     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19960920)51:6<613::AID-BIT1>3.0.CO;2-K     Document Type: Article

References (34)

1. Acsadi, G., Jani, A., Massie, B., Simoneau, M., Holland, P., Blaschuk, K., Karpati, G. 1994. A differential efficiency of adenovirus-mediated in vivo gene transfer into skeletal muscle cells of different maturity. Hum. Molec. Genet. 3: 579-584.
2. Acsadi, G., Massie, B., Jani, A. 1995. Adenovirus-mediated gene transfer into striated muscles. J. Mol. Med. 73: 165-180.
3. Berkner, K. L. 1988. Development of adenovirus vectors for the expression of heterologous genes. BioTechniques 6: 616-629.
4. Berkner, K. L. 1992. Expression of heterologous sequences in adenoviral vectors. Curr. Top. Microbiol. Immunol. 153: 39-66.
5. Bibila, T. A., Ranucci, C. S., Glazomitsky, K., Buckland, B. C., Aunins, J. G. 1994. Monoclonal antibody process development using medium concentrate. Biotechnol. Prog. 10: 87-96.
6. Boucher, R. C., Knowles, M. R. 1994. Gene therapy for cystic fibrosis using E1-deleted adenovirus: a phase 1 trial in the nasal cavity. Hum. Gene Ther. 5: 615-634.
7. Butler, M., Jenkins, H. 1989. Nutritional aspects of the growth of animal cells in culture. J. Biotechnol. 12: 97-110.
8. Chua, F. K. F., Yap, M. G. S., Oh, S. K. W. 1994. Hyper-stimulation of monoclonal antibody production by high osmolarity stress in eRDF medium. J. Biotechnol. 37: 265-275.
9. Crystal, R. G., Mcelvaney, N. G., Rosenfeld, M. A., Chu, C. S., Mastrangeli, A., Hay, J. G., Brody, S. L., Jaffe, H. A., Eissa, N. T., Danel, C. 1994. Administration of an adenovirus containing the human CFTR cDNA to the respiratory tract of individuals with cystic fibrosis. Nature Genet. 8: 42-51.
10. Gaertner, J. G., Dhurjati, P. 1993. Fractional factorial study of hybridoma behavior. 1. Kinetics of growth and antibody production. Biotechnol. Prog. 9: 298-308.
11. Gaertner, J. G., Dhurjati, P. 1993. Fractional factorial study of hybridoma behavior. 2. Kinetics of nutrient uptake and waste production. Biotechnol. Prog. 9: 309-316.
12. Garnier, A., Côté, J., Nadeau, I., Kamen, A., Massie, B. 1994. Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells. Cytotechnology 15: 145-155.
13. Glacken, M. W. 1988. Catabolic control of mammalian cell culture. Bio/ Technology 6: 1041-1050.
14. Glacken, M. W., Fleischaker, R. J., Sinskey, A. J. 1986. Reduction of waste product excretion via nutrient control: possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells. Biotechnol. Bioeng. 28: 1376-1389.
15. Graham, F. L., Smiley, J., Russel, W. C., Nairn, R. 1977. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36: 59-74.
16. Hassel, T., Gleave, S., Butler, M. 1990. Growth inhibition in animal cell culture. Appl. Biochem. Biotechnol. 30: 29-40.
17. Hiller, G. W., Aeschlimann, A. D., Clark, D. S., Blanch, H. W. 1991. A kinetic analysis of hybridoma growth and metabolism in continuous suspension culture on serum-free medium. Biotechnol. Bioeng. 38: 733-741.
18. Kamen, A., Tom, R., Caron, A., Chavarie, C., Massie, B., Archambault, J. 1991. Culture of insect cells in a helical ribbon impeller bioreactor. Biotechnol. Bioeng. 38: 619-628.
19. Kay, M. A., Woo, S. L. C. 1994. Gene therapy for metabolic disorders. Trends Genet. 10: 253-257.
20. Kurokawa, H., Park, Y. S., Lijima, S., Kobayashi, T. 1994. Growth characteristics in fed-batch culture of hybridoma cells with control of glucose and glutamine concentrations. Biotechnol. Bioeng. 44: 95-103.
21. Kyung, Y.-S., Peshwa, M. V., Gryte, D. M., Hu, W.-S. 1994. High density culture of mammalian cells with dynamic perfusion based on on-line oxygen uptake rate measurements. Cytotechnology 14: 183-190.
22. Ljunggren, J., Haggstrom, L. 1994. Catabolic control of hybridoma cells by glucose and glutamine limited fed batch cultures. Biotechnol. Bioeng. 44: 808-818.
23. Massie, B., Dionne, J., Lamarche, N., Fleurent, J., Langelier, Y. 1995. Improved adenovirus vector provides herpes simplex virus ribonucleotide reductase R1 and R2 subunits very efficiently. Bio/Technology 13: 602-608.
24. Miller, W. M., Wilke, C. R., Blanch, H. W. 1989. Transient responses of hybridoma cells to nutrient additions in continuous culture: 1. Glucose pulse and step changes. Biotechnol. Bioeng. 33: 477-486.
25. Oh, S. K. W., Vig, P., Chua, F., Teo, W. K., Yap, M. G. S. 1993. Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate. Biotechnol. Bioeng. 42: 601-610.
26. Oyaas, K., Ellingsen, T. E., Dyrset, N., Levine, D. W. 1994. Hyperosmotic hybridoma cell cultures: increased monoclonal antibody production with addition of glycine betaine. Biotechnol. Bioeng. 44: 991-998.
27. Ozturk, S. S., Palsson, B. O. 1991. Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production. Biotechnol. Bioeng. 37: 989-993.
28. Ozturk, S. S., Riley, M. R., Palsson, B. O. 1991. Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production. Biotechnol. Bioeng. 39: 418-431.
29. Trapnell, B. C. 1993. Adenoviral vectors for gene transfer. Adv. Drug Deliv. Rev. 12: 185-199.
30. Trapnell, B. C., Gorziglia, M. 1994. Gene therapy using adenoviral vectors. Curr. Opin. Biotechnol. 5: 617-625.
31. Xie, L. Z., Wang, D. I. C. 1994. Fed-batch cultivation of animal cells using different medium design concepts and feeding strategies. Biotechnol. Bioeng. 43: 1175-1189.
32. Zabner, J., Couture, L. A., Gregory, R. J., Graham, S. M., Smith, A. E., Welsh, M. J. 1993. Adenovirus-mediated gene transfer transiently corrects the chloride transport defect in nasal epithelia of patients with cystic fibrosis. Cell. 75: 207-216.
33. Zhou, W., Hu, W.-S. 1994. On-line characterization of a hybridoma cell culture process. Biotechnol. Bioeng. 44: 170-177.
34. Zhou, W., Rehm, J., Hu, W.-S. 1995. High viable cell concentration fed-batch culture of hybridoma cells through on-line nutrient feeding. Biotechnol. Bioeng. 46: 579-587.