Isolation of Novel Pelagic Bacteria from the German Bight and Their Seasonal Contributions to Surface Picoplankton

Applied and Environmental Microbiology

Volumn 67, Issue 3-12, 2001, Pages 5134-5142

  Eilers, Heike ^a   Pernthaler, Jakob ^a   Peplies, Jörg ^a   Glöckner, Frank Oliver ^b   Gerdts, Gunnar ^b   Amann, Rudolf ^a  

^a MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY   (Germany)

^b ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHAPROTEOBACTERIA; BACTEROIDETES; CYTOPHAGA; CYTOPHAGA SP.; FLAVOBACTERIUM; GAMMAPROTEOBACTERIA; ROSEOBACTER;

RIBOSOME DNA; RNA 16S; SEA WATER;

ANIMAL; ARTICLE; BACTERIAL COUNT; BACTERIUM; CLASSIFICATION; DNA SEQUENCE; FLUORESCENCE IN SITU HYBRIDIZATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR GENETICS; PHYLOGENY; PLANKTON; SEASON;

ANIMALS; BACTERIA; COLONY COUNT, MICROBIAL; DNA, RIBOSOMAL; IN SITU HYBRIDIZATION, FLUORESCENCE; MOLECULAR SEQUENCE DATA; PHYLOGENY; PLANKTON; RNA, RIBOSOMAL, 16S; SEASONS; SEAWATER; SEQUENCE ANALYSIS, DNA;

EID: 0035514014     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.67.11.5134-5142.2001     Document Type: Article

References (50)

1. Amann, K. I., L., Krumholz, and D. A. Stahl. 1990. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172:762-770.
2. Bernard, L., H. Schäfer, F. Joux, C. Courties, and G. Muyzer. 2000. Genetic diversity of total, active and culturable marine bacteria in coastal seawater. Aquat. Microb. Ecol. 23:1-11.
3. Brosius, J., T. J. Dull, D. D. Sleeter, and H. F. Noller. 1981. Gene organization and primary structure of a ribosomal RNA operon from Eschcrich:a coli. J. Mol. Biol. 148:107-127.
4. Buchan, A., L. S. Collier, E. L. Neidle, and M. A. Moran. 2000 Key aromaticring-cleaving enzyme, prolocatechuate 3,4-dioxygenase, in the ecologically important marine Roseobacter lineage. Appl. Environ. Microbiol. 66:4662-4672.
5. Buchholz-Cleven, B., B. Rattunde, and K. Straub. 1997. Screening for genetic diversity of isolates of anaerobic Fe(II)-oxidizing bacteria using DGGE and whole-cell hybridization. Syst. Appl. Microbiol. 20:301-309.
6. Button, D. K., B. R. Robertson, P. W. Lepp, and T. M. Schmidt. 1998. A small, dilule-cytoplasm, high-affinity, novel bacterium isolated by extinction culture and having kinetic constants compatible with growth at ambient concentrations of dissolved nutrients in seawater. Appl. Environ. Microbiol. 64:4467-4476.
7. Button, D. K., K. Schut, P. Quang, R. Martin, and B. Robertson. 1993. Viability and isolalion of marine bacteria by dilution culture: theory. procedures, and initial results. Appl. Environ. Microbiol. 59:881-891.
8. Cottrell, M. T., and D. L. Kirchman. 2000. Community composition of marine bacterioplankton determined by 16S rRNA gene clinic libraries and fluorescence in situ hyhridization. Appl. Environ. Microbiol. 66:5116-5122.
9. Cottrell, M. T., and D.L. Kirchman. 2000. Natural assemblages of marine prolcobacteria and members of the Cytophaga-Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter. Appl. Environ. Microbiol. 66:1692-1697.
10. DeLong, E. F., D. G. Franks, and A. L. Alldredgr, 1993 Phylogenetic diversity of aggregate-attached vs. free-living marine bacterial assemblages, Limnol. Occanogr 38:924-934.
11. DeLong, E. F., L. T. Taylor, T. L. Marsh, and C. M. Preston. 1999 Visualization and enumeration of marine planktonic archaea and bacteria by using polyribonucleotide probes and fluorescent in situ hybridization. Appl. Environ. Microbiol. 65:5554-5563.
12. Eberl, L., S. Molin, and M. Givskos. 1999. Surface motility of Serratia liquefactens MGI. Bacteriol. 181:1703-1712.
13. Eilers, H., J. Pernthaler, and R. Amann. 2001. Succession of pelagic marine bacteria during enrichment: a close look at cultivation-induced shifts. Appl. Environ. Microbiol. 66:4634-4640.
14. Ellers, H., J. Pernthaler, K. O. Glöckner, and K. Amann. 2000. Culturability and in situ abundance of pelagic bacteria from the Nonrht Sea. Appl. Environ. Microbiol. 66:3044-3051.
15. Ferguson, R. L., E. N. Buckley, and A. V. Palumba. 1984. Response of marine bacterioplankton to differential filtration and confinement Appl. Environ. Microbiol. 47:49-55.
16. Glöckner, F. O., R. Amann, A. Alfreider, J. Pernthaler, R. Psenner, K. Trebeslus, and K.-H. Schleifer. 1996. An inn situ hyhridization protocol for detection and identification of planklonic bacteria Syst Appl. Microbiol. 19:403-436.
17. Glöckner, F. O., B. M. Fuchs, and R. Amann. 1999. Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl. Environ. Microbiol 65:.3721-3726.
18. Gonzalez, J. M., R. P. Kiene, and M. A. Moran. 1999. Transformation of sulfur compounds by an abundant lineage ol marine bacteria in the αa-subclass of the class Protcobacteria Appl. Environ. Microbiol. 65:3810-3819.
19. Gonzalez, J. M., and M. A. Moran. 1997. Numerical dominance of a group of marine bacteria in the α-subclass of the class Protcobacteria in coastal seawater. Appl. Environ. Microbiol. 63:4237-4242.
20. Gonzalez, J. M., and M. A. Moran. 1997. Numerical dominance of a group of marine bacteria in the α-subclass of the class Protcobacteria in coastal seawater. Appl. Environ. Microbiol. 63:4237-4242.
21. Gonzalez, J. M., K. Simo, K. Massana, J. S. Cosert, E. O. Casamayor, C. Pedros-Alio, and M. A. Moran. 2000 Bacterial communm structure associated with a dimethylsalfoniopropionate-producing North Atlantic algal bloom. Appl. Environ. Microbiol. 66:4237-4246.
22. Gonzalez, J. M., W. B. Whitman, R. E. Hodson, and M. A. Moran. 1996. Identifying numerically abundant culturable bacteria from complex communities: an example Irom a lignin enrichment culture. Appl. Environ. Microbiol. 62:4433-4441.
23. Hagmeier, E.1998. Norsecberichl 1998: Phytoplankton der HelgolandReede, Landesamt fur Natur und Umwelt des Lindes Schleswig-Holstein. Flintbek, Germany.
24. Hobbie, J. E., R. J. Dairy, and S. Jasper. 1977. Use of Nuclepore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33:1225-1228.
25. Holmström, C., and S. Kjclleberg. 1999. Marine Pseudoalteromonas species are associated with higher organisms and produce biologically active extra-cellular agents. FEMS Microbiol Ecol. 30:285-293.
26. Jannasch, H. 1967. Ennchments ot aqualic bacteria in continuous culture. Arch. Mikrobiol. 59:165-173.
27. Karner, M., E. F. DeLong, and D. M. Karl. 2001. Archaeal dominance in the mesopelagic zone of the Pacific Ocean. Nature 409-507-509.
28. Karner, M., and J. A. Fuhrman. 1997. Delermination of active marine bacteroplankton: a comparison of universal 16S rRNA probes, autoradiography. and nucleoid staining. Appl. Environ. Microbiol. 63:1208-1213.
29. Labrenz, M., M. D. Collins, P. A. Lawson, H. J. Tindall, P. Schumann, and P. Hirsch. 1999. Roscovarius tolearans gen nov., sp. nov. a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int. J. Syst. Bacteriol. 49:137-147.
30. Manz, W., R. Amann, W. Ludwig, M. Vancanneyt, and K.-H. Schleifer. 1996. Application of a suite of 16S rRNA-specitic oligonucleotide probes designed to investigate bacteria of tile phylum Cytophaga- Fiavobacter-Bacteroides in the natural environment. Microbiology 142:1097-1106.
31. Manz, W., R. Amann, W. Ludwig, M. Wagner, and K.-H. Schleifer. 1992. Phylogenetic oligodcoxynuclcolide probes for the major subclasses of Proteobactena - problems and solutions. Syst. Appl. Microbiol. 15:593-600.
32. Montanari, M. P., C. Pruzzo, L. Pane, and R. R. Colwell. 1999. Vibrios associated with plankton in a coastal zone of the Adriatic Sea (Italy) FEMS Microbiol. Ecol. 29:241-247.
33. Neef, A. 1997. Ph.D. thesis. Technical University. Munich. Germany.
34. Neef, A., A. Zaglauer, H. Meier, R. Amann, H. Lemmer, and K. H. Schleifer. 1996. Population analysis in a denitrifying sand filter: conventional and in situ identification of Paracoccus spp. in methanol-led biofilms. Appl. Environ. Microbiol. 62:4329-4339.
35. Ouverney, C. C., and J. A. Fuhrman. 2001. Marine planktonic Archaea take up amino acids. Appl Environ. Microbiol. 66:4829-4833.
36. Pernthaler, J., F. O. Glockner, S. Unterholzner, A. Alfreider, R. Psenner, and R. Amann. 1998. Seasonal community and population dynamics ot pelagic Bacteria and Archaea in a high mountain lake. Appl. Environ. Microbiol. 64:4299-4306.
37. Pinhassi, J., and A. Hagström. 2000. Seasonal succession in marine bacterioplankton. Aquat. Microb. Ecol. 21:245-256.
38. Pinhassi, J., U. L. Zweifel, and A. Hagström. 1997. Dominant marine bacterioplankton species found among colony-forming bacteria. Appl Environ Microbiol. 63:3359-3366.
39. Prokic, I., F. Brummer, T. Brigge, H. D. Grortz, G. Gerdts, C . Schult. M. Elbrachter, and W. E. G. Muller. 1998. Bacteria of the genus Roscobacter associated with the toxic dinoflagellate Prorocenitrom fona. Protist 149:347357.
40. Radach, G., J. Berg, and E. Hagmeier. 1990. Long-term changes of the annual cycles ot meteorological, hydrographic, nutrient, and phytoplankton times series at Helgoland and at L.V Elbe 1 in the German Bighl. Continental Shelf Res. 10:.305-328.
41. Kappé. M. S., P. F. Kemp, and S. J. Giovannoni. 1997. Phylogenetic diversity of marine coastal picoplankton 16S rRNA genes cloned from the continental shelf off Cape Hatleras. North Carolina. Limnol. Oceanogr. 42:811-826.
42. Saiki, R. K., D. H. Gelfand, S. Stofel, S. J. Scharf, R. Higuchi, G. T. Horn, K. B. Mulliris, and H. A. Erlich. 1998. Primer-directed enzymalic amplificalion of DNA with a thermostable UNA polymerase. Science 239:487-491.
43. Schäfer, H. L. Bernard, C. Courtits, P. Lebaron, P. Senats. K. Pukall, E. Slacktbrandt, M. Trousstllier, T. Guindulain, J. Vives-Rego, and G. Muyzer. 2001. Microbial community dynamics in Mediterranean nutrientenriched seawater, FEMS Microbiol. Ecol. 34::243-253.
44. Schut, F., E. J. De Vries, J. C. Gottschal, H. R. Roberlson, W. Harder, R. A. Prins, and D. K. Bution. 1993. Isolation of typical marine bacteria by dilution culture growth maintenance and characteristics ol isolates under laboratory conditions. Appl. Environ. Microbiol. 59:2150-2160.
45. Simon, M., F. O. Glöckner, and R. Amann. 1999. Different community structure and temperature optima of heterotrophic picoplankton in various regions of UK Southern Ocean. Aqual. Microb. Ecol. 18:275-284.
46. Straskrabvá, V. 1983. The effect of substrate shock on populations of starving aqualic bacteria. J. Appl. Bacteriol. 54:217-224.
47. Suzuki, M. T., L. T. Taylor, and E. F. DeLong. 2000. Quantilative analysis of small-suhunit rRNA genes in mixed microbial populations via 5′-nuclease assays. Appl. Environ. Microbiol. 64:4605-4614.
48. Thingstad, T. K. and R. Lignell. 1997. Thenretical models for the control of bacterial growth rate, abudance. diversity and carbon demand. Aquat. Microb. Ecol. 13:19-27.
49. ZoBell, C'. E. 1446. Marine microbiology. A monograph on bydrobacteriology. Chronica Botanica Company. Waltham. Mass.
50. Zubkov, M. V., B. M. Fuchs, S. D. Archer, R. P. Kitne, R. Amann, and P. A. Hurkill. 2001. Linking the composition of bactcrioplankton to rapid turnover of dissolved dimethylsultoniopropionatc in an algal bloom in the North Sea. Environ Microbiol 3:304-311.