Dissecting the translocase and integrase functions of the Escherichia coli SecYEG translocon

Journal of Cell Biology

Volumn 150, Issue 3, 2000, Pages 689-694

  Koch, H G ^a   Muller, M ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Membrane proteins; Protein transport signal recognition particle; SecA; SecYEG complex

Indexed keywords

BACTERIAL ENZYME; CARRIER PROTEIN; INTEGRASE; MEMBRANE PROTEIN; SIGNAL RECOGNITION PARTICLE;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ESCHERICHIA COLI; MEMBRANE VESICLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT;

ADENOSINE TRIPHOSPHATASES; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CELL MEMBRANE; ESCHERICHIA COLI PROTEINS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE SYSTEM; SIGNAL RECOGNITION PARTICLE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA;

EID: 0034618026     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.150.3.689     Document Type: Article

References (34)

1. In vitro membrane assembly of a polytopic, transmembrane protein results in an enzymatically active conformation
2. FtsH (HflB) is an ATP-dependent protease selectively acting on SecY and some other membrane proteins
3. Characterization of cold sensitive secY mutants of Escherichia coli
4. A cytoplasmic domain is important for the formation of a SecY-SecE translocator complex
5. Discrimination between SRP- and SecA/SecB-dependent substrates involves selective recognition of nascent chains by SRP and trigger factor
6. Requirements for the translocation of elongation-arrested, ribosome-associated OmpA across the plasma membrane of Escherichia coli
7. Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle
8. Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli
9. Biogenesis of the gram negative bacterial envelope
10. SecA suppresses the temperature-sensitive secY24 defect in protein translocation in Escherichia coli membrane vesicles
11. Comparative characterization of secA from the α subclass purple bacterium Rhodobacter capsulatus and Escherichia coli reveals differences in membrane and precursor specificity
12. Identification of a soluble SecA/SecB-complex by means of a subfractionated cell-free export system
13. Subunit interactions in the Escherichia coli protein translocase: SecE and SecG associate independently with SecY
14. In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli
15. An alternative protein targeting pathway in Escherichia coli: Studies on the role of FtsY
16. The functional integration of a polytopic membrane protein of Escherichia coli is dependent on the bacterial signal recognition particle
17. The pKSM710 vector cassette provides tightly regulated lac and TTlac promoters for manipulating N-terminal protein sequences
18. SecY and SecA interact to allow SecA insertion and protein translocation across the Escherichia coli plasma membrane
19. In vitro translocation of bacterial proteins across the plasma membrane of Escherichia coli
20. A mutation in the Escherichia coli secY gene that produces distinct effects on inner membrane protein insertion and protein export
21. Disruption of the gene encoding p12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature
22. Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation
23. Gene fusion analysis of membrane protein topology: A direct comparison of alkaline phosphatase and β-lactamase fusions
24. SecA is required for the insertion of inner membrane proteins targeted by the Escherichia coli signal recognition particle
25. One of the three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery
26. FtsY, the prokaryotic signal recognition particle receptor homologue, is essential for biogenesis of membrane protein
27. Coupled structure changes of SecA and SecG revealed by the synthetic lethalitiy of the secAcsR11 and ΔsecG::kan double mutant
28. Biochemical analysis of the biogenesis and function of the Escherichia coli export factor SecY
29. Protein translocation functions of Escherichia coli SecY: In vitro characterization of cold-sensitive secY mutants
30. Insertion of the polytopic membrane protein MalF is dependent on the bacterial secretion machinery
31. The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins
32. The Escherichia coli SRP and SecB targeting pathways converge at the translocon
33. Membrane insertion of the mannitol permease of Escherichia coli occurs under conditions of impaired SecA function
34. Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli