Vectors to express foreign genes and techniques to monitor gene expression in Pseudomonads

Current Opinion in Biotechnology

Volumn 12, Issue 5, 2001, Pages 439-445

  Schweizer, Herbert P ^a  

^a Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAY; BIOSENSOR; EXPRESSION VECTOR; GENE CONSTRUCT; GENE CONTROL; GENE EXPRESSION REGULATION; GENE TARGETING; GENE TECHNOLOGY; LACTOSE OPERON; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS; REPORTER GENE; REVIEW; TRANSPOSON;

NEGIBACTERIA; PSEUDOMONAS;

EID: 0035476701     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(00)00242-1     Document Type: Review

References (47)

1. Complete nucleotide sequence and gene organization of the broad-host-range plasmid RSF1010
2. Molecular genetics of broad host range plasmid RK2
3. Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and the sequence of the region required for their replication in Pseudomonas aeruginosa
4. Development of broad-host-range vectors and gene banks: Self-cloning of the Pseudomonas aeruginosa PAO chromosome
5. Construction and characterization of a highly regulable expression vector, pLAC11, and its multipurpose derivatives, pLAC22 and pLAC33
6. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative bacteria
7. FACS-optimized mutants of the green fluorescent protein (GFP)
8. Bacterial bioluminescence: Organization, regulation, and application of lux genes
9. Complete genome sequence of Pseudomonas aeruginosa, an opportunistic pathogen
10. pUCP-Nco and pUCP-Nde: Escherichia-Pseudomonas shuttle vectors for recombinant protein expression in Pseudomonas
11. Heterologous expression in Pseudomonas aeruginosa of the 9.2-kDa c-type cytochrome subunit of p-cresol methylhydroxylase
12. Utilization of mini-Dlac transposable element to create an α-complementation and regulated expression system for molecular cloning in Pseudomonas aeruginosa
13. Reporter gene vectors and assays
14. DNA supercoiling and environmental regulation of gene expression in pathogenic bacteria
15. Designing microbial systems for gene expression in the field
16. Specialized host-vector systems for the engineering of Pseudomonas strains destined for environmental release
17. New broad-host-range promoter probe vectors based on the plasmid RK2 replicon
18. Transcription reporters that shuttle cloned DNA between high-copy Escherichia coli plasmids and low-copy broad-host-range-plasmids
19. Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5 and Tn10-derived transposons
20. Regulation of quorum sensing by RpoS in Pseudomonas aeruginosa
21. Engineering a mouse metallothioneine on the cell surface of Ralstonia eutropha CH34 for immobilization of heavy metals in soil
22. Detection and quantification of tetracyclines by whole biosensors
23. Versatile biosensor vectors for detection and quantification of mercury
24. A bioluminescent derivative of Pseudomonas putida KT2440 for deliberate release into the environment
25. Molecular tools for study of biofilm physiology
26. Design of bacterial hosts for lac-based expression vectors
27. Integration-proficient Pseudomonas aeruginosa vectors for isolation of single copy chromosomal lacZ and lux gene fusions
28. Integration proficient plasmids for Pseudomonas aeruginosa: Site-specific integration and use for engineering of reporter and expression strains
29. Molecular analysis of a cytotoxin-converting phage, φCTX, of Pseudomonas aeruginosa: Structure of the attP-cos-ctx region and integration into the serine tRNA gene
30. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally located DNA sequences: Application for isolation of unmarked Pseudomonas aeruginosa mutants
31. Vector design and host systems for Pseudomonas
32. Construction of improved vectors for protein production in Pseudomonas aeruginosa
33. pBluescript II: Multifunctional cloning and mapping vectors
34. A two-component T7 system for the overexpression of genes in Pseudomonas aeruginosa
35. L promoter of coliphage λ: Regulated synthesis of human interleukin 2 in Erwinia and Serratia species
36. R promoters by a cI repressor in a broad-host-range thermoregulated plasmid marker system
37. Broad-host-range expression vectors containing manipulated meta-cleavage pathway regulatory elements of the TOL plasmid
38. q/Ptrp-lac plasmids and transposons that confer conditional phenotypes
39. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector
40. Construction and use of a versatile set of broad-host-range cloning and expression vectors based on the RK2 replicon
41. Improved broad-host-range RK2 vectors for high and low regulated gene expression levels in Gram-negative bacteria
42. Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria
43. Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene
44. Construction of a modified mini-Tn5 luxCDABE for the development of bacterial biosensors for ecotoxicity testing
45. BAD promoter
46. Loss of Pseudomonas aeruginosa PhpA aminopeptidase activity results in increased algD transcription