Versatile biosensor vectors for detection and quantification of mercury

FEMS Microbiology Letters

Volumn 193, Issue 1, 2000, Pages 123-127

  Hansen, Lars Hestbjerg ^a   Sørensen, Søren Johannes ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

gfp; lacZ; lux; Mercury; Soil contamination; Whole cell biosensor

Indexed keywords

BETA GALACTOSIDASE; GENE PRODUCT; GREEN FLUORESCENT PROTEIN; MERCURY;

ARTICLE; BIOSENSOR; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE CASSETTE; GENE FUSION; GENE TARGETING; GENE TRANSFER; GRAM NEGATIVE BACTERIUM; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS PUTIDA; REGULATOR GENE; REPORTER GENE; SOIL POLLUTION; TRANSPOSON;

BACTERIAL PROTEINS; BIOSENSING TECHNIQUES; CLONING, MOLECULAR; DNA TRANSPOSABLE ELEMENTS; DNA-BINDING PROTEINS; GENES, BACTERIAL; GENES, REGULATOR; GENES, REPORTER; GENETIC VECTORS; MERCURY; PROMOTER REGIONS (GENETICS); PSEUDOMONAS PUTIDA; SOIL POLLUTANTS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS PUTIDA;

EID: 0034562560     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00462-6     Document Type: Article

References (26)

1. Bacterial biosensors for monitoring toxic metals
2. A single-use luciferase-based mercury biosensor using Escherichia coli HB101 immobilized in a latex copolymer film
3. Application of a mer-lux biosensor for estimating bioavailable mercury in soil
4. Bioluminescent sensors for detection of bioavailable mercury(II) in the environment
5. Luminescence facilitated detection of bioavailable mercury in natural waters
6. A mer-lux transcriptional fusion for real-time examination of in vivo gene expression kinetics and promoter response to altered superhelicity
7. Detection of DNA damage by use of Escherichia coli carrying recA'::lux, uvrA'::lux, or alkA'::lux reporter plasmids
8. A chromosomally based tod-luxCDABE whole-cell reporter for benzene, toluene, ethylbenzene, and xylene (BTEX) sensing
9. Assessment of bioavailability of heavy metals using lux modified constructs of Pseudomonas fluorescens
10. Development of an acute and chronic ecotoxicity assay using lux-marked Rhizobium leguminosarum Biovar trifolii
11. Green fluorescent protein-based reporter systems for genetic analysis of bacteria including monocopy applications
12. Chromosomal insertion of the entire Escherichia coli lactose operon, into two strains of Pseudomonas, using a modified mini-Tn5 delivery system
13. Improved single and multicopy lac-based cloning vectors for protein and operon fusions
14. Site-specific deletions of chromosomally located DNA segments with the multimer resolution system of broad-host-range plasmid RP4
15. A general system to integrate lacZ fusions into the chromosomes of Gram-negative eubacteria: Regulation of the Pm promoter of the TOL plasmid studied with all controlling elements in monocopy
16. Specific-purpose plasmid vectors II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas
17. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli
18. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in Gram-negative bacteria
19. Conjugation in aquatic environments
20. DNA replication and the division cycle of Escherichia coli
21. Cell-density-dependent sensitivity of a mer-lux bioassay
22. Induction of bacterial mercury- and copper-responsive promoters - Functional differences between inducible systems and implications for their use in gene-fusions for in vivo metal biosensors
23. Green fluorescent protein-marked Pseudomonas fluorescens: Localization, viability, and activity in the natural barley rhizosphere
24. Simultaneous monitoring of cell number and metabolic activity of specific bacterial populations with a dual gfp-luxAB marker system