Construction and characterization of a highly regulable expression vector, pLAC11, and its multipurpose derivatives, pLAC22 and pLAC33

Plasmid

Volumn 44, Issue 2, 2000, Pages 138-151

  Warren, James W ^a   Walker, Jennifer R ^a   Roth, John R ^c   Altman, Elliot ^b  

^a UNIVERSITY OF GEORGIA   (United States)

^b University of Georgia   (United States)

^c UNIVERSITY OF UTAH   (United States)

Author keywords

Cloning; Inducible; Plasmid; Repressible

Indexed keywords

ARTICLE; CELL GROWTH; ESCHERICHIA COLI; EXPRESSION VECTOR; LACTOSE OPERON; MOLECULAR CLONING; NONHUMAN; PLASMID; PROTEIN EXPRESSION;

ESCHERICHIA COLI;

EID: 0033812363     PISSN: 0147619X     EISSN: None     Source Type: Journal    
DOI: 10.1006/plas.2000.1477     Document Type: Article

References (37)

1. Characterization of a region in mature LamB protein that interacts with a component of the export machinery of Escherichia coli
2. ATG vectors for regulated high-level expression of cloned genes in Escherichia coli
3. Tightly regulated tac vectors useful for the expression of unfused and fused proteins in Escherichia coli
4. Design and construction of expression plasmid vectors in Escherichia coli
5. Plasmid vector pBR322 and its special-purpose derivatives - A review
6. Synthesis, cloning, and expression in Escherichia coli of a spinach acyl carrier protein-I gene
7. Construction and characterization of new cloning vehicles II. A multipurpose cloning system
8. Expression vectors employing lambda-, trp-, lac-, and lpp-derived promoters
9. Regulation of ribosomal RNA promoters with a synthetic lac operator
10. DNA sequence for a low-level promoter of the lac repressor gene and an 'up' promoter mutation
11. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli
12. Chemical synthesis and expression of a synthetic gene for the flavodoxin from Clostridium MP
13. cDNA cloning, expression, and rapid purification of a Kunitz-type winged bean chymotrypsin inhibitor
14. An over-expression plasmid for Escherichia coli primase
15. The effects of mutations in the ant promoter of Phage P22 depend on context
16. Identification of a sex-factor-affinity site in E. coli as γδ
17. Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin
18. High copy number of the pUC plasmid results from a Rom/ Rop-suppressible point mutation in RNA II
19. An E. coli gene product required for lambda site-specific recombination
20. Lac repressor and lac operator
21. High level expression of rat gamma-D-crystallin in Escherichia coli
22. Overproduction of the Bacillus sphaericus R modification methylase in Escherichia coli and its purification to homogeneity
23. McrA and McrB restriction phenotypes and some E. coli strains and implications for gene cloning
24. Cloning of the triosephosphate isomerase gene of Plasmodium falciparum and expression in Escherichia coli
25. Purification and characterization of glutathione reductase encoded by a cloned and over-expressed gene in Escherichia coli
26. A collection of strains containing genetically linked altering antibiotic resistance elements for genetic mapping of Escherichia coli
27. Use of bacteriophage-T7 lysozyme to improve an inducible T7 expression system
28. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
29. Use of T7 RNA-polymerase to direct expression of cloned genes
30. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers
31. Direct selection of mutations reducing transcription or translation of the recA gene of Escherichia coli with a recA-lacZ protein fusion
32. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp 18 and pUC19 vectors
33. Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene