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Controls for the aldehydes and ketones were not performed because we had previously found that those used were not cytotoxic. In principle, these could be performed by assessing the cytotoxicity at the same single value (5 μM) used for the library.
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note
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Random sampling of 32 wells revealed that 60% were essentially composed of only the expected chalcone; of the other 40% most were mixtures of the benzaldehyde, acetophenone, and chalcone.
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