Protocols for high efficiency, stage-specific retroviral transduction of murine fetal thymocytes and thymic epithelial cells

Journal of Immunological Methods

Volumn 253, Issue 1-2, 2001, Pages 209-222

  Travers, Helen ^a   Anderson, Graham ^a   Gentle, Dean ^a   Jenkinson, Eric ^a   Girdlestone, John ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

CD80; GFP; Organ culture; Retrovirus; Thymus

Indexed keywords

ADENOVIRUS; ANIMAL CELL; ANTIGEN PRESENTING CELL; ARTICLE; CELL DIFFERENTIATION; CELL MATURATION; CONTROLLED STUDY; EPITHELIUM CELL; FETUS CELL; GENETIC TRANSDUCTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; RETROVIRUS; T LYMPHOCYTE; TECHNIQUE; THYMOCYTE; VIRAL GENETICS; VIRUS INFECTION;

ANIMALS; ANTIGEN PRESENTATION; ANTIGENS, CD80; EPITHELIAL CELLS; GENES, BCL-2; MICE; MICE, INBRED BALB C; MICE, TRANSGENIC; ORGAN CULTURE TECHNIQUES; RETROVIRIDAE; T-LYMPHOCYTES; THYMUS GLAND; TRANSDUCTION, GENETIC; TRANSGENES;

MURINAE; UNIDENTIFIED RETROVIRUS;

EID: 0035400273     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(01)00391-X     Document Type: Article

References (30)

1. + thymocytes in vitro
2. Differential effects of peptide diversity and stromal cell type in positive and negative selection in the thymus
3. Studies on thymic epithelial cells in vitro
4. Impaired fetal thymocyte development after efficient adenovirus-mediated inhibition of NF-κB activation
5. A simple and efficient method for the concentration and purification of recombinant retrovirus for increased hepatocyte transduction in vivo
6. High-efficiency retroviral-mediated gene transfer into human and nonhuman primate peripheral blood lymphocytes
7. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells
8. B7-1 overexpression by thymic epithelial cells results in transient and long-lasting effects on thymocytes and peripheral T helper cells but does not result in immunodeficiency
9. Targeting rare populations of murine antigen-specific T lymphocytes by retroviral transduction for potential application in gene therapy for autoimmune disease
10. The MAP kinase pathway controls differentiation from double-negative to double positive thymocyte
11. Expression of Bcl-X(L) restores cell survival, but not proliferation and effector differentiation, in CD28-deficient T lymphocytes
12. Mice lacking major histocompatibility complex class I and class II molecules
13. Green fluorescent protein retroviral vectors: Low titer and high recombination frequency suggest a selective disadvantage
14. Identification of a developmentally regulated phase of postselection expansion driven by thymic epithelium
15. In vitro models of T cell development
16. Establishment of efficient reaggregation culture system for gene transfection into immature T cells by retroviral vectors
17. Signaling checkpoints during the development of T lymphocytes
18. Transcriptional regulation of T lymphocyte development and function
19. CD28/B7 system of T cell costimulation
20. Role of the guanosine triphosphatase rac2 in T helper 1 cell differentiation
21. Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection
22. Impaired negative selection in CD28-deficient mice
23. Distinct roles of the phosphatidylinositol 3-kinase and STAT5 pathways in IL-7-mediated development of human thymocyte precursors
24. A novel method of cell separation based on dual parameter immunomagnetic cell selection
25. Production of high-titer helper-free retroviruses by transient transfection
26. Increased probability of expression from modified retroviral vectors in embryonal stem cells and embryonal carcinoma cells
27. Targeted complementation of MHC class II deficiency by intrathymic delivery of recombinant adenoviruses
28. bcl-2 inhibits multiple forms of apoptosis but not negative selection in thymocytes
29. Development of retrovirally marked human T progenitor cells into mature thymocytes
30. An improved retroviral gene-transfer technique demonstrates inhibition of CD4-CD8-thymocyte development by kinase-inactive ZAP-70