Role of the guanosine triphosphatase Rac2 in T helper 1 cell differentiation

Science

Volumn 288, Issue 5474, 2000, Pages 2219-2222

  Li, Baiyong ^a,d   Yu, Hong ^a   Zheng, Wei Ping ^a,e   Voll, Reinhard ^a,f   Na, Songqing ^a,g   Roberts, Andrew W ^b,h   Williams, David A ^b   Davis, Roger J ^c   Ghosh, Sankar ^a   Flavell, Richard A ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^d PFIZER INC   (United States)

^e UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^f UNIVERSITY HOSPITAL ERLANGEN   (Germany)

^g ELI LILLY AND COMPANY   (United States)

^h WALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

GAMMA INTERFERON; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; ISOENZYME; PROTEIN KINASE; RAC PROTEIN; TETRACYCLINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; INTERFERON PRODUCTION; LYMPHOCYTE DIFFERENTIATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; TH1 CELL; TRANSGENIC MOUSE;

ANIMALS; CELL DIFFERENTIATION; CELLS, CULTURED; CYTOKINES; GENE EXPRESSION REGULATION; HUMANS; INTERFERON TYPE II; JNK MITOGEN-ACTIVATED PROTEIN KINASES; JURKAT CELLS; LYMPHOCYTE ACTIVATION; MICE; MICE, TRANSGENIC; MITOGEN-ACTIVATED PROTEIN KINASES; NF-KAPPA B; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PROMOTER REGIONS (GENETICS); RAC GTP-BINDING PROTEINS; SIGNAL TRANSDUCTION; TH1 CELLS; TRANSFECTION;

ANIMALIA; MURINAE; MUS MUSCULUS;

EID: 0034705628     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5474.2219     Document Type: Article

References (30)

1. W. E. Paul and R. A. Seder, Cell 76, 241 (1994).
2. M. Hubank and D. G. Schatz, Nucleic Acids Res. 22, 5640 (1994).
3. W. Zheng and R. A. Flavell, Cell 89, 587 (1997).
4. A. Minden, A. Lin, F. X. Claret, A. Abo, M. Karin, Cell 81, 1147 (1995).
5. O. A. Coso et al. Cell 81, 1137 (1995).
6. R. Perona et al., Genes Dev 11, 463 (1997).
7. M. Rincón et al., EMBO J. 17, 1817 (1998).
8. D. D. Yang et al., Immunity 9, 575 (1998).
9. M. Rincón and R. A. Flavell, EMBO J. 13, 4370 (1994).
10. Chop-luciferase assay kit from Stratagene was used as directed by the manufacturer.
11. R. J. Phillips, S. Gustafson, S. Ghosh, Mol. Cell. Biol. 16, 7089 (1996).
12. B. Li and R. Flavell, unpublished data.
13. T. M. Aune, L. A. Penix, M. R. Rincón, R. A. Flavell, Mol. Cell. Biol. 17, 199 (1997).
14. M. K. Jenkins and R. H. Schwartz, J. Exp. Med. 165, 302 (1987).
15. J. Kaye, S. Porcelli, J. Tite, B. Jones, C. A. Janeway Jr., J. Exp. Med. 158, 836 (1983).
16. Constitutively active IKKβ and the NFκB super repressor (a dominant-negative IκBα) were generated through site-directed mutagenesis.
17. C. Tournier, A. J. Whitmarsh, J. Cavanagh, T. Barrett, R. J. Davis, Proc. Natl. Acad. Sci. U.S.A. 94, 7337 (1997).
18. J. Raingeaud, A. J. Whitmarsh, T. Barrett, B. Derijard, R. J. Davis, Mol. Cell. Biol. 16, 1247 (1996).
19. P. Lores, L. Morin, R. Luna, G. Gacon, Oncogene 15, 601 (1997).
20. M. Gossen et al., Science 268, 1766 (1995).
21. P. Shockett, M. Difilippantonio, N. Hellman, D. G. Schatz, Proc. Natl. Acad. Sci. U.S.A. 92, 6522 (1995).
22. 1 mice were used for experiments.
23. Total RNA was isolated with Trizol reagent (Gibco-BRL). The mCK-1 multiprobe template sets (Phar-Mingen) was used for RNase protection assay.
24. The Phoenix-Eco packaging cell line was transfected with retroviral vector according to the protocol developed by G. P. Nolan (www.stanford.edu/group/ nolan/NL-helper.html). Viral supernatant was harvested 48 hours after transfection and was used to infect activated primary T cells in the presence of polybrene (Sigma) at 37°C for 24 hours. Cells were then washed, supplied with fresh media, and further cultured for 5 days before analysis.
25. A. W. Roberts et al., Immunity 10, 183 (1999).
26. H. Yu and R. Flavell, unpublished data.
27. H. Lu et al., EMBO J. 18, 1845 (1999).
28. K. Reif and D. A. Cantrell, Immunity 8, 395 (1998).
29. S. Kaga, S. Ragg, K. A. Rogers, A. Ochi, J. Immunol. 160, 24 (1998).
30. We thank A. Hall for providing the expression vectors for Rac1N17, Rac1L61, CDC42N17, and CDC42L61; Genetics Institute for IL-12; T. Hirano for the ETS binding sites-luciferase construct; R. Breese for assistance in maintaining Rac mouse stocks; J. Stein for technical support; and F. Manzo for manuscript preparation. W.-p.Z. and B.L. were associates, H.Y. is an associate, D.A.W. and S.G. are associate investigators, and R.J.D. and R.A.F. are investigators of the Howard Hughes Medical Institute.